laser scanning confocal microscopy 中文意思是什麼

laser scanning confocal microscopy 解釋
激光共焦點掃描顯微鏡
  • laser : n 鐳射激光，受激發射光，激光；萊塞；激光器，光激射器 （ ＝ light amplification by stimulated emis...
  • scanning : n. 1. 細看，細察，審視。2. 【電視】掃描，掃掠,搜索。
  • confocal : adj. 【數學】共焦（點）的。
  • microscopy : n. 顯微鏡學；顯微鏡使用術。
  1. Clsm confocal laser scanning microscopy

    共焦激光掃描顯微鏡
  2. Semiquantitative analysis of lignin content in the culm by confocal laser scanning microscopy ( clsm ) showed that lignin contents are elevated in lodging - resistant varieties and this together with the anatomical features may provide higher culm strength resulting in increased lodging - resistance

    因此，在超高產小麥品種的選育上，重視外源基因的引進，改進莖稈的結構特性及提高其木質素含量，可能是一個重要的選育方向。
  3. It was showed under the laser scanning confocal microscopy that : for dna level fish, 81 % of the dnas were in the nucleoli and at the periphery of the nucleoli and 19 % in the nucleoplasm ; for rna level fish, 22 % of the rnas were in the nucleoli, 78 % in the nucleoplasm and at the boundary between it and the nucleoli ; for dna - rna level fish, 25 %, 46 % and 29 % of the dnas or rnas were in the nucleoli, at the periphery of the nucleoli and in the nucleoplasm, respectively

    結果如下： dna水平熒光原位雜交結果顯示， 81的dna位於核仁內部及其核仁周邊區域， 19的位於核質中； rna水平熒光原位雜交結果表明， 22的rna位於核仁內， 78的位於核質及與核仁交界處； dna - rna水平熒光原位雜交結果是， 25的dna或rna位於核仁內， 46處于核仁周邊， 29位於核質中。由此推測出， rna聚合酶的轉錄主要發生在核仁及其周邊區域。
  4. We used fission yeast schizosaccharomyces pombe ( s. pombe ), an unicellular eukaryotic organism, as research material. electroporation was adopted to load ca2 + fluorescent indicator into yeast cell and under the laser scanning confocal microscopy ( lscm ), we observed cytosolic ca2 + distribution and relative content as well as fluorescence intensity of gfp - cam in different phases of cell cycle of yeast cell. flow cytometry provided a way of determining the relative dna content of populations of fission yeast

    本文以單細胞的真核模式生物裂殖酵母（ schizosaccharomycespombe ）為研究材料，通過激光掃描共聚焦顯微鏡觀察酵母細胞胞質內游離ca ~ （ 2 + ）的分佈及相對濃度，以及不同周期時相細胞中gfp - cam的熒光強度變化，並採用細胞流式法對酵母細胞的相對dna含量進行測定以確定細胞所處周期時相。
  5. By sds - page and immuno - blotting, we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen. under confocal laser scanning microscopy after immunoflurescence labeling, we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube

    免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現，類細胞質力蛋白中間鏈在百合花粉管中存在於顆粒狀細胞器上；免疫熒光雙標及激光共聚焦掃描顯微鏡觀察發現，百合花粉管中類細胞質力蛋白中間鏈和微管存在部分共分佈。
  6. It also showed good cell affinity to the modified pdl - la scaffold investigated by scanning electronic microscopy ( sem ) and confocal laser scanning microscopy ( clsm ), comparing with the unmodified pdl - la scaffold

    掃描電鏡和激光共聚焦顯微鏡的測試結果顯示了軟骨細胞在原浙江大學博士學位論文（ 2003 ）位自修飾的聚乳酸三維支架內部良好的粘附與生長情況。
  7. Using confocal laser scanning microscopy ( clsm ), we observed the distribution of chimeric protein in different kinds of tissues and cells in transgenic toreniafournieri

    經激光共聚焦顯微鏡觀察了轉基因植株的各種不同組織中融合蛋白的表達和分佈情況。
  8. After treatment with bfa, immunoflurescence labeling and confocal laser scanning microscopy indicated that the cytoplasmic dynein intermediate chain - like protein in lily pollen tube was insensitive to bfa treating, but spectrin - like protein was sensitive to it under the same condition

    用bfa處理百合花粉管后，通過免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現類細胞質力蛋白中間鏈對bfa不敏感；同樣的處理發現類紅膜肽對bfa敏感。
  9. It was showed under the laser scanning confocal microscopy that the transfected plasmid dna transcripted by rna polymerase and its transcripts were both localized in the interior and periphery of nucleoli

    由此我們認為rna聚合酶的轉錄就是發生在這一區域里，而並非是人們傳統認識中的核質中。
  10. The lung tissue for immunohistochemitry and laser scanning confocal microscopy were fixed and embedded. the morphological alteration of pulmonary neuroendocrine cells which stain for calcitonin gene - related peptide ( cgrp ), serotonin ( s - ht ) and luteinizing hormone ( lh ) were studied. the results of these were dealed with computer image analysis and statistical treatment

    肺組織取材后經固定、梯度酒精脫水、包埋、連續切片后，應用免疫組織化學方法、透射電鏡及激光掃描共聚焦技術觀察了降鈣素基因相關肽（ cgrp ） 、五羥色胺（ 5 - ht ） 、黃體生成素（ lh ）陽性細胞的隨齡變化，並對實驗結果進行了計算機圖像分析和統計學處理。
  11. In this paper, we introduce the function of the confocal laser scanning microscopy in the studies of protein crystal growth kinetics, such as measuring the numbers of crystal, reconstruction 3 - d image to acquire the information of crystal growth process

    本文介紹了其在定量測量晶體的個數，重組三維圖像以獲得晶體生長的過程信息及測定晶體生長臺階動態變化等方面的應用。
  12. So it is necessary to examine calcium activity and distribution in nerve cells. a way of visualizing intracellular ca2 + in three dimensional was established by using laser scanning confocal microscopy ( lscm ) and computer visualization technique in this paper. based on this way, which includes cell culturing and dyeing, confocal microscopy optimizing, confocal data preprocessing, 3d visualization of ca2 + by computer, we investigated the ca2 * distribution in cultured hippocampal neurons under different objectives

    本文通過激光掃描共聚焦顯微技術和計算機三維可視化技術建立了一套神經細胞內鈣離子分佈三維可視化的方法，包括細胞的培養和染色、顯微鏡參數的優化、共聚焦數據的預處理、針對鈣離子的三維可視化方法的實現，為胞內鈣離子作用機制的研究提供直觀的手段。
  13. The homepage provides the information of research interests, details of seminar schedules ( date, speaker, title ), course descriptions, research facilities ( includes cell & molecular imaging facility, laser scanning confocal microscopy and so on ), and links to university of north carolina medicine department, centers and programs, curricula, related center and program ( includes bowels center for alcohol studies, lineberger comprehensive cancer center, cell and molecular biology trainning program, center for gastrointestinal biology and disease, department of ophthalmology and so on )

    中文簡介:查珀爾希爾北卡羅來納大學醫學院細胞和發育生物學系的主頁提供研究方向信息，講座日程安排的詳情（日期，發言者，標題） ，課程描述，研究設施（細胞、分子成像設備，激光掃描共焦顯微鏡等等） ，與北卡羅來納大學醫學系，中心，項目，課程，相關中心與計劃（酒精研究內臟中心，林內貝格綜合癌癥中心，細胞與分子與生物訓練計劃，腸胃生物疾病中心，眼科系）的鏈接。
  14. Here, aba - induced h2o2 generation and their roles in inducing stomatal closing were investigated by epidermal strips bioassay, laser scanning confocal microscopy and patch clamp with ios5 and its wild type plant as materials. there were no clear differences between wild type and ios5 in the density, shapes and aperture of their stomata

    本文以los5及其相應野生型為材料，採用表皮條生物分析、激光掃描共聚焦顯微技術及膜片鉗技術研究了nacl脅迫條件下， aba誘導保衛細胞內h _ 2o _ 2的產生及二者在誘導氣孔關閉中的作用。
  15. Laser scanning confocal microscopy ( lscm ) is now established as a valuable tool for obtaining high resolution images of a variety of biological specimens

    激光共聚焦掃描顯微鏡能對生物樣本進行高解析度三維層切掃描，故廣泛應用於生物、醫學等研究領域。
  16. H2o2 generation in guard cells was examined by laser scanning confocal microscopy based on fluorescence probe h2dcfda. the fluorescence intensity in guard cells of wild type and ios5 was essentially the same before treatment

    以h2o2熒光探針h2dcfda結合激光掃描共聚焦顯微技術直接檢測了保衛細胞內的h2o2產生情況。未加任何處理之前，野生型與los5保衛細胞內熒光強度幾乎相等。
  17. Furthermore, when 10 imo il h2o2 ( final concentration ) was added to the suspension after above treatment caused little reduction in hpts fluorescence. the results obtained by laser scanning confocal microscopy suggested that addition of exogenous aba resulted in a rapid decrease in fluorescence in most cellular compartments of the guard cells

    維生素c可部分逆轉低濃度h2o2 （ 10 - 5 ）所誘導的氣孔關閉過程：並且10 - 6mol l的dpi和103u ml的過氧化氫酶（ cat ）在一定程度上也逆轉了aba誘導張開氣孔的關閉。
  18. This new method will bring significant developments in studying the principles of stomatal movement, and other quick movement in plants, c ) guard cells are incubated with ph dependent fluorescent chemical probe " bcecf am " and excited at 488nm, the fluorescent emission ratio method ( 520nm / 640nm ) is employed with laser scanning confocal microscopy, about 0. 4 ph unit increase in guard cell vacuoles is observed during stomatal closure that is induced by aba

    本發展為保衛細胞與其它小細胞液泡的進一步研究提供了新思路。 c ）本工作通過激光共聚焦顯微術配合ph熒光探針bcecfam的單激發（ 488nm ）雙發射的熒光比值法（ 525nm 640nm ）觀察到，用aba處理的表皮條上的開放態氣孔在關閉過程中其保衛細胞液泡內ph有一約0 . 4單位的上升。
  19. Based on epidermal strip bioassay, microinjection, patch - clamp and laser scanning confocal microscopy in the experiments, we provided the first evidence that map kinases, including mek1 / 2 or p38 / hog1, plays an important role i n aba - or sa - induced h2o2 signal initial, amplification and specific targeting in response to stimuli in guard cells. aba - or h2o2 - induced vicia faba stomatal closure. was inhibited or reversed by the specific inhibitor pd98059 of mek1 / 2 ; the guard cells were pre - incubated or - microinjected by 10 umol l - 1 pd98059, aba could not enhance the fluorescence intensity of h2o2 probe dichlorofluorescein ( dcf )

    在對照實驗中， aba誘導熒光迅速增高；單獨的pd98059 、 pd98059和aba共同處理氣孔時，保衛細胞內h _ 2o _ 2探針熒光強度沒有增高；將pd98059注射進入其中的一個保衛細胞，再以aba處理，使得兩個有同樣熒光基礎的保衛細胞熒光強度對比強烈；將pd98059顯微注射進入已被aba誘導dcf （ dichlorofluorescin ）熒光強度升高的氣孔保衛細胞，熒光強度下降，而沒有被注射一邊的保衛細胞中的dcf熒光強度不變。
  20. Confocal laser scanning microscopy ( clsm ), atomic force microscopy [ afm ] [ and transmission verified the occurrence of the deposition

    Hrp通過自發沉積技術被包埋到了微膠囊中；激光共聚焦顯微鏡、原子力顯微鏡和透射電鏡觀察證明了hrp的自發沉積。
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