level flask 中文意思是什麼
level flask
解釋
水準瓶-
The transfermants with highest resistance to g418 ( 4 g / l in ypd ) were screened to study their expression level in 150 ml shaking flask. having been induced with methanol for 36 hours, the target enzyme could be examined in the supernatant by measuring amidolytic activity
首次將大連蛇島賅蛇毒類凝血酶成熟基回克隆到表達載體ppicgk中，經電激轉化至畢氏酵母菌株gs15中，再經甲醇誘導，在150ml搖瓶畔1獲得細胞外分泌表達產物。 -
The yeast strain 12y - 5 was identified at the species level using two yeast taxonomy systems published by. j. w. kreger - van rij ( 1984 ) [ 24 ] and j. a. barnett ( 1983 ). [ 19 ] in addition, the ability to assimilate several compounds as main sources of carbon and nitrogen was evaluated at the 500ml shake - flask and 3. 7l kfl bioreactor scales. effects of ph, temperature, concentration of dissolved oxygen and utilizable sugar on the growth rate of cells and the rate of the conversion of substrate into biomass were studied
進而用正交試驗法對該酵母生長的培養基配方及影響生長的重要理化因素進行了較為深入的研究，在此基礎上對影響生長的重要理化因素進行了寬范圍的測驗：並以搖瓶最佳的培養條件為基礎，在3 . 7升kfl -生物反應器上進行了放大試驗。 -
Under the induction by methanol, the expressed monellin was secreted into medium at the level of 0. 25g / l. the gene ( vgb ) coding for the vitreoscilla hemoglobin ( vhb ) molecule was cloned and funtionally expressed in pichia pastoris. shake flask fermentation experiments showed that the presence of the intracellular vhb in pichia pastoris efficiently enhanced cell growth under oxygen - poor conditions
同時，採用畢赤酵母偏愛密碼子，人工合成了透明顫菌血紅蛋白基因（ vgb ） ；並構建了vgb基因的酵母胞內表達載體ppic3 . 5kv ，通過電擊轉化獲得了能在胞內表達有生物活性vhb的重組畢赤酵母gs115 / ppic3 . 5kv 。
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