lexa 中文意思是什麼
lexa
解釋
萊克薩-
In this study, lexa mutant of deinococcus radiodurans xe has been successfully constructed. the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different times of mmc
本研究利用已構建的抗輻射菌lexa基因突變體菌株xe ，經受不同劑量射線照射和不同時間的mmc作用，平板菌落計數，繪制存活曲線。 -
In this study, dna strand breaks in jm109 cells caused by - rays and the protective effect of lexa protein of deinococcus radiodurans have been studied by fluorometric assay of dna unwinding., the results indicated that the rate of dna strand breaks increased with the increase of - rays dose. however, when the concentration of lexa protein from 0. 1 g / ml to 10 g / ml, it still showed no protective effect on dna strand breaks, then lexa protein of deinococcus radiodurans was n ' t radioprotector
本研究採用了dna解旋熒光法檢測由射線所致的dna鏈斷裂的損傷程度以及抗輻射菌lexa蛋白的輻射防護作用，在輻照前加入不同濃度的lexa蛋白，檢測dna鏈的斷裂，結果表明dna鏈的斷裂程度隨著射線輻照劑量的增加而增強，抗輻射菌lexa蛋白對射線所致dna鏈斷裂的修復不起作用，進一步說明抗輻射菌lexa蛋白不起輻射防護作用。 -
The expression of lexa protein in the iptg - induced jm109 ( pza172 ) was checked by sds - page, and the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different concentrations of mmc
應用電穿孔技術將攜帶有抗輻射菌lexa基因的重組質粒pza172轉入大腸桿菌jm109 ，其啟動子為lacz ，用iptg誘導， sds - page凝膠電泳檢測lexa蛋白的表達。 -
When compared with kds301 ( lexa + ), xe ( lexa - ) cells showed quite similar levels of survival curve to y - irradiation and mmc, indicating lexa gene had no effect on radiation resistance of deinococcus radiodurans. deinococcus radiodurans " lexa had no revelance with its extremely radioresistance. the recombinant plasmid pza172 was constructed by cloning the lexa gene of deinococcus radiodurans into the vector plasmid puc19 in the downstream of lacz promoter
對lexa基因突變體xe的研究表明，其輻射抗性與lexa基因野生型的抗輻射菌kd8301相近，存活曲線上均顯示有一個寬大的肩區，結果發現lexa基因的突變並沒有改變抗輻射菌的獨特抗性，說明lexa基因與輻射抗性無關。 -
This resistance is known to be due to deinococcus radiodurans " extremely proficient dna repair pathway employed by this organism. also, the exsistence of sos response and characterization of deinococcus radiodurans lexa protein remains unclear
抗輻射菌中是否存在sos修復路徑，及在大腸桿菌等菌中廣泛存在的lexa蛋白在抗輻射菌中的功能特性如何均是未解之謎。 -
After iptg induction, the lexa protein was significantly expressed in the strain jm109 ( pza172 ), and the survival rate of this strain was lower than that of the control strain jm109
抗輻射菌lexa基因在大腸桿菌細胞中，經iptg誘導能穩定地表達， lexa基因的顯著表達降低了大腸桿菌經射線照射和mmc作用后的存活率。 -
The expression of deinococcus radiodurans lexa gene in escherichia coli resulted in the increase of sensitivity of the latter to dna toxins y - rays and mmc, although the mechanism of this effect is not clear
抗輻射菌lexa基因在大腸桿菌中的顯著表達降低了大腸桿菌對射線和mmc的抗性，其作用機理還有待于進一步的研究。
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