linked gene 中文意思是什麼

linked gene 解釋
連鎖基因
  • linked : 聯動的
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  1. Screening target ' gene of artemisinin antimalarials using drug - western from cdna expressing library : 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether was linked to bsa by using of edc cross ! inker and the product acted as drug - probe

    對重組pmd一18一t克隆載體及pqe一30表達載體雙酶切，提取tctp基因和pqe一30空載體並使二者重組，然後轉化m15 ，挑取陽
  2. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  3. Conclusion the variation of dna sequences of coagulase gene is probably linked to vancomycin - resistance in s. aureus

    結論凝固酶基因酶切位點的改變及其他位點堿基的改變，可能是凝固酶活性降低的原因之一。
  4. Sub - - clone of s, . / hbsag fusion gene : pbuescripts, . / hbsag and ppiczaa were digested separately by xhoi and xbai enzyme, and were linked under t4 dna ligase, ppiczaa s, / hbsag was constructed and transformed to e. coli

    Hbsag質粒與ppiczaa載體分別經xhol和xbaln切，再在t4dna連接酶作用下進行連接，獲得工程菌表達型ppiczaas ； hbsag質粒，轉化大腸桿菌t0p10細胞，經xhol和xbal與sacll和xbal酶切電泳，證實s ； 。
  5. At first. then eight a - amylase gene fragments were cloned with the genomic dnas as templates by routine pcr. following that, these gene fragments and plasmid vectors, pbluescript ii ks + and puc18, were cut by bamh i and kpn i. the prepared insert dna and vector dna were linked by t4 dna ligase

    利用vectornti6 . 0軟體，對所克隆的序列用相鄰接點法（ neighborjoining州j ） method ）進行多序列比對，分析其同源性，並構建基因進化樹。
  6. A group of related and closely linked structural genes, together with the operator gene that controls their expression are collectively called an operon.

    一組相關的、緊密連鎖的結構基因加上控制其表現的操縱基因，統稱操縱子。
  7. Some nine human number have been shown through family studies to be linked to the peptidase gene.

    通過人的家族研究發現約有9個基因與肽酶基因是連鎖的。
  8. 7. at the first time, the reporter dye, fam was linked to the 5 " - end of the oligonucleotides of the probes, and the tamra was located at the 3 " - end as quencher dye. we use camv35s and fmv promoter, nos terminater, mark gene nptii, and aim gene pat, epsps and cryla ( b ) genes as target sequences, design pairs of sp

    7 、首次以fam熒光素標記探針5 』端作為發光基團，以tarma標記探針3 』端為淬滅基團，以camv35s 、 fmv啟動子、 nos終止子、標記基因nptll 、抗除草劑基因epsps 、 pat 、抗蟲基因cry1a ）為檢狽目標，設計、篩選出特異性引物和探針，優化實驗參數，建立了轉基因植物通用性熒光pcr定性檢測方法體系。
  9. The extracellular 100pl ( ecl1 ) and ecl2 was linked by disulfide bond to maintenanced the stability of the protein secondary structure. in recent years, we showed that ccr5 as a co - receptor could interact with hiv - 1 infect ion. ccr5 was paid closed attention to since it was cloned in 1996. the aim is to - obtain the sequence of first extracillular domain of 3 chemokine receptor 5 ( ccr5 ) n - terminal gene fragment with high level expression in e. coli and to prepare its specific antibody f ( ab ' ) ;, and its detected method

    Ccr5具有g蛋白偶聯受體家族（ g - proteincoupledreceptors ， gpcrs ）所特有的7個跨膜區（ transmembrinedomains ， tm ） ，呈螺旋， tm的氨基酸有很高的保守性，膜外第一襻（ extracellularloop1 ， ecl1 ）和ecl2之間有二硫鍵相連，以維持蛋白質二級結構的穩定性。
  10. They said they found that progesterone, which is involved in the female reproductive cycle, stimulates the proliferation of breast cells carrying a gene linked to breast cancer, possibly feeding tumor growth

    他們說他們發現黃體酮，這一參與婦女生殖周期的激素，刺激攜帶與乳癌有關的基因的乳腺細胞的增殖。
  11. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  12. This gene has been studied for years, but scientists did not know it was linked to itching

    事實上科學家已經研究這個基因很久了，但一直沒有想到它與癢覺會有聯系。
  13. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  14. It is suggested that 538bp sequence of ast gene 5 " end had been cloned after the 138bp fragment was linked up with the 706bp fragment. the analysis of 538bp sequence with the software of promoter prediction indicated that there maybe exist four transcriptional initiator sites, one caat - box and two gc - boxes

    將該片段與706bp的片段對接后，表明克隆到了ast基因的上游啟動子的538bp序列，通過promoterpredict軟體進行啟動子的分析，顯示該序列存在可能的四個轉錄起始位點，一個caat框和兩個gc框。
  15. Incompletely linked gene

    不完全連鎖基因
  16. We have made comparisons among current methods for gene mapping. most methods based on linkage analysis and association analysis considers one marker at a time and does not take into account the correlated structure of multiple linked markers

    我們對比了現有的基因定位分析方法，基於連鎖分析和關聯分析的基因定位方法多是針對單個疾病特徵標記來研究，忽略了多個可能疾病特徵標記間復雜互作的綜合效應。
  17. Ln our experiment, a lot of regulator sequences inc1uding the 35s promoter with double enhancer e1ements and the terminator nos, the fl sequence and kozak sequence for improving the genetic statement in the level of translation, were appended on the two s ides of the artifically pseudopleuronectes americanus antifreeze protei n gene by a series of process, and the products were linked into pbil21

    本實驗中人工合成了美洲擬鰈抗凍蛋白基因，並在美洲擬鰈抗凍蛋白基因兩側添加了許多調控序列，如具有加倍增強子的35s啟動子和nos終止子，它們可以促進抗凍蛋白基因的轉錄，及可在翻譯水平上提高基因表達的序列和kozak序列，從而構建了抗凍蛋白基因的高效表達盒。
  18. It was also proved that the biosynthestic genes of apramycin was linked to the apramycin resistant gene in s. tenebrarius

    Pcr實驗證明基因重組菌株中接合轉移質粒同源整合到黑暗鏈黴菌h6的染色體上。
  19. In this study, we constructed two plant expression vector with rip gene from barley and linked to two promoters which have high promoting efficiency in monocotyledonous plants, respectively, and then transferred to rice mediated by agrobacterium tumefaciens. the main factors of affecting transformation were investigated and established its regeneration and transformation systems. pcr analysis showed primarily that rip gene had already integrated into rice

    本研究構建了分別由單子葉植物中啟動效率較高的pact和p2 35s啟動子調控的大麥核糖體失活蛋白基因（ rip ）的植物表達載體，通過農桿菌介導法轉化水稻，經pcr檢測初步驗證了rip基因已導入水稻。
  20. This study raises hope that they might have another option - - using drugs rather than surgery to guard against cancers linked to the mutated gene

    這一研究使他們有了另一個選擇的希望?應用藥物而不是手術來對抗因基因突變而致的癌癥。
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