liquid culture 中文意思是什麼

liquid culture 解釋
液體培養
  • liquid : n 1 液體。2 【語言學】流音〈如 [l] [r]〉 adj 1 液體的，液態的，流動的。2 清澄的，透明的。3 易變的...
  • culture : n. 1. 教養；修養；磨煉。2. 文化，（精神）文明。3. 人工培養，養殖；培養菌，培養組織。4. 耕作；栽培；造林。vt. 使有教養。
  1. The primary results showed : using m199 as diluents containing 20 % bovine serum, it is better to freeze the cells slowly freezing at fist then increase freezing speed ( for example, from 0 to - 6 freezing speed is about - 0. 05 a minute, from - 6 to - 40, freezing speed is about - 0. 5 a minute ), studies on effect of various concentration of dmso demonstrate that about 12. 5 % dmso gave the highest post - thaw percentage of viable cells. the concentration of bovine serum had no different effect on the percentage of the viable embryo cells of misgurnus auguillicaudatus. the embryo cells derived 6 from the later stage of blastula offish is more resistant to the cryogen than the cells of early stage of blastula. the cells preserved in liquid nitrogen at - 196 were thawed and cultivated, a few cells were found adhere to the surface of culture vessel when the percentage of viable cell was more than 30 %. the cells in only two culture vessels were found to proliferated and gave rise to many small morphologically undifferentiated cells

    研究初步表明:以細胞培養液m199 （含2既的小牛血清，常規量雙抗）為凍存稀釋液對泥鰍胚胎細胞冷凍保存宜採取先慢后快的方式（例如，從0一一6 ，凍存速度為一0 . 05 / min ，再以一0 . 5 / min的速度從一6一一40 ） ; dmso的保護效應濃度為12 . 506左右;小牛血清的濃度對泥鰍胚胎細胞的成活率影響不明顯;囊胚晚期細胞抗凍性比中早期強;通過對不同批次的凍存細胞解凍培養，解凍后成活率為30 %以上細胞培養數天後均有少數細胞貼壁，但只發現兩瓶培養細胞有明顯增殖現象產生許多未分化的小細胞。
  2. Steamed millets with water content of 45 % ( broomcorn ) and 42 % ( foxtail ) were inoculated with liquid culture of p. delphacis ( containing mycelial mass of ~ 25 mg / ml ) at a ratio of 20 % ( v / w ) and then incubated at 25 ? and l : d 12 : 12

    將菌絲生物量約為25mg / ml的菌絲液按20的比例（ v / w ）接入經高溫濕熱滅菌並適度熟化、含水量分別為45和42的黍米及粟米中，在25和12l ： 12d條件下直接培養，所獲3 17d黍米和粟米培養物的產孢潛能和有效產孢時間因培養天數不同而異。
  3. Hydroponics ( water culture ) the growth of plants in liquid culture solutions rather than soil

    水培：是在液體培養基中栽培植物而不是在土壤中。
  4. This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan

    在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。
  5. The effect of chestnut putamina on three sorts of edible fungi prepared by liquid submerged - culture

    板栗殼對三種食用菌液體培養的影響
  6. Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus

    本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。
  7. Monokaryons of this fungus will grow readily as saprophytes in plate or liquid culture.

    這種真菌的單核體在平板或液體培養時能像腐生菌那樣容易生長。
  8. Ounce glass of water is recommended. also drinking hot tea any type with a little lemon will help. the liquid and heat soothe and hydrate4 the throat while the lemon acts as an astringent to shrink swollen tissues. if a sore throat lasts more than a couple of days see a doctor for a throat culture to rule out strep throat

    最好在一杯8盎司的水中放半勺鹽。另外，喝加了少許檸檬的熱茶任何茶也會有所幫助。液體和熱氣會減輕嗓子痛，給嗓子提供水份，而檸檬則起到收斂劑的作用，使腫起的組織收縮。
  9. The tolerance of suillus granulatus and paxillus involutus to zn, cd, and pb was then compared using liquid culture medium without agitation. compared to suillus granulatus, paxillus involutus was more tolerant to zn and pb, but more sensitive to cd than suillus granulatus. the adsorption of heavy metals in ectomycorrhizal fungi might be related to the base exchange capacity of the fungal mycelium

    在此基礎上，研究了兩種外生菌根真菌對不同濃度重金屬cd和zn的耐性能力，發現不同真菌對不同重金屬的耐性不同，表現在卷緣樁菇對zn的耐性高於點柄乳牛肝菌，而兩種真菌對cd的耐性則相反。
  10. Medium experiments were arranged under uniform design, and then an optimum medium was got accordingly. the culture liquid was centrifugalized at 3, 500r / min for 30min, then ammonium sulfate was added into the supernatant to a final concentration of 30 % to precipitate the others

    通過硫酸銨分級沉澱、 deaesephadexa - 50陰離子交換凝膠層析和sephadexg - 75凝膠柱層析對發酵液進行分離和純化，並得到電泳純的酶。
  11. It was found that b - 6 isolated by the method of distinct zone of clearing in cellulose - congo red agar medium combined with measuring the enzyme activity of liquid culture filtrates had comparatively higher cellulase activity. by measuring activity of cellulase of strains growing in medium with different carbon sources and of washed mycelium induced by different carbon sources in certain time, it found that the formation of cellulase was regulated by the nature of the carbon source used for b - 6 and as3. 3711

    真菌纖維素酶是一種誘導酶，碳源同時也是主要的誘導物來源，為了研究碳源對真菌纖維素酶合成的誘導機理，本文利用液體生長培養和洗滌菌絲誘導培養法研究了不同碳源對兩菌株的誘導特性，並用電泳分析法研究了不同碳源的誘導酶譜。
  12. Edible gelatine. detection of coliforms. 30 degrees c culture method on liquid selective medium

    食用明膠.大腸桿菌的檢測.在30時液體選擇培養基的培養法
  13. Edible gelatine. determination of fecal coliforms. 44, 5 degrees c culture method on liquid selective medium

    食用明膠.糞便中大腸桿菌的檢測.在44 . 5時液體選擇培養基的培養法
  14. After co - cultivation, all the explants were washed in liquid mso medium containing 500mg / l cef for 2 hours. washed with sterile water for 5 times and patted dry on the filter paper again. subsequently, the explants were transferred to ms1 medium supplemented with 200mg / l cef for 3 - 6 days delay selection and then transferred to ms1 medium supplemented with 200mg / l cef and 50mg / l km for selection culture

    對普通pcr檢測兩種結果均為陽性的轉化株系，又同時以355和nptll為目的片段進行2xmpcr檢測，結果得到tg一rch卜n二l一20 （表示檢測結果為陽性的轉基因矮牽牛rch卜有加個株系） 、 te一rch ，一n = z一10和to一wch ， + n = l一10 。
  15. The most common means of agitation of liquid media is by placing the culture vessels on a drum (for test tubes).

    液體培養基最普通的搖動方式，要把培養器皿放置於圍繞一根近水平的軸緩慢旋轉的圓筒（用於試管）。
  16. A substance extracted from mycelia with benzinum was studied for the first time. five compositions were found : butylated hydroxytoluene, n - hexadcanoic acid, 9, 12 - octadecadienoic acid methyl ester, eicosane and hexacosane. the paper provided scientific basis data for researching mainly biologic characteristics and active compositions of mycelia from phellinus igniarius cultured with solid and liquid culture media

    首次對菌絲體的石油醚提取物進行定性分析，確定該菌絲體中含有二丁基夯基甲苯、棕桐酸、 9 ， 12一十八碳二烯酸甲醋，二十碳烷及二十六碳烷，這五種物質均為在桑黃菌絲體中首次發現。
  17. In the present thesis, physical and nutrient factors that affect the growth of s. platensis ag were studied systematically for the purpose of increasing the biomass of strain a9 in liquid culture

    本文對影響鈍頂螺旋藻a _ 9株生長的理化因子進行了系統的研究。
  18. This paper studied the nutrient physiological characteristics and the liquid culture condition of cg rather completely

    本論文採用各種試驗方法對土生空團菌的營養生理特性和液體培養條件進行了比較全面的研究。
  19. In order to investigate the tolerance of ectomycorrhizal fungi to heavy metals in vitro, three culture methods, namely liquid culture without agitation, liquid culture with agitation and solid agar culture, were investigated to determine which method would give the best combination of fungal biomass and ec50. the results indicated that liquid medium without agitation was the best culture method

    為研究外生菌根真菌本身對重金屬污染的耐性，比較了液體靜置、液體搖床和瓊脂固體培養這三種常用的菌絲體的純培養方法，以真菌生物量大小和分離難易程度為主要指標，篩選出液體靜置方法為最優方法。
  20. According to morphologic characteristics. when cultured in liquid medium, cadmium with initial concentration below 4. 61mg / l did n ' t inhibited the growth of f2, 163. 8mg / l of cadmium affected it obviously but growth continued to a great extent. cadmium removal from medium by f2 under liquid culture was not so stable with low cadmium concentration below 4. 61mg / l, but removal ratio reached 96 % with initial cadmium concentration of 163. 8mg / l and cadmium content of biomass reached 28 %, which showed high capacity of cadmium accumulation by f2

    F2在液體培養時，培養基初始鎘濃度在4 . 61mg l以下時對其生長無抑制作用，鎘濃度為163 . 8mg l時有較大影響，但仍有明顯生長； f2對液體培養基中低濃度鎘（ 4 . 61mg l以下）的去除效果不太穩定，但初始鎘濃度為163 . 8mg l時，其去除率為96 ，菌體最大鎘含量可達28 ，顯示了較大的富集容量。
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