low molecular weight protein 中文意思是什麼
low molecular weight protein
解釋
低分子量蛋白-
Constituting 60 % of total plasma protein, and with low molecular weight, albumin contributes about 80 % of the colloid osmotic pressure
因其占血清總蛋白質的60 %和低分子量的特性，白蛋白司職約80 %的膠體滲透壓。 -
It was found that prophenoloxidase ( propo ), isolated as a monomeric protein, had a molecular weight of 87. 5 kda, and a 77 kda phenoloxidase molecule was often contained in preparations. the 87. 5 kda propo had a very low enzymatic activity on 0. 02 mol / l l - dopa, whereas the 77 kda po had a very high enzymatic activity on it
實驗發現，酚氧化酶原（ prophenoloxidase ）的分子量為87 . 5kda左右，在實驗操作過程中極易發生降解或自身降解，變成有活性的大小約77kda的酚氧化酶， 1 sds可使該酶原全部被激活而成為有活性的酚氧化酶。 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
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