lysogen 中文意思是什麼
lysogen
解釋
溶菌原-
In addition, no back mutation to obtain a maximal complexity ( i. e. - resistant and sensitive species coexistence ) or original ecology ( i. e. original - lysogen ) for the evolution occurs
而且，逆反應變異回原有菌相而成對噬菌體敏感及阻抗菌相共存之最大分歧度或回歸至起始單一對噬茵體敏感菌相併未曾演化發生。 -
Addition of iptg to growing culture of the lysogen induces t7 rna polymerase, which in turn transcribe the target dna in the plasmid. in the presence of glucose and appropriate conditions such as temperature and concerntration of iptg, a 52kd protein with tryptopanase activity was expressed
摸索發酵條件，如改變培養溫度和iptg濃度等，發現在30培養條件下， 0 . 2mmiptg誘導時，發酵液中的吲哚含量最高，表明低濃度的誘導劑或低溫誘導有利於表達出有活性的色氨酸酶。 -
To prove that the cloned dna fragment can express tryptopanase, a new plasmid pet28c - tnaa, in which the cloned dna fragment was located downstream of t7 promoter on pet28c was constructed and transformed into host bl21 ( de3 ), a bl21 lysogen of bacteriophage de3 in which the only promoter known to direct transcription of the t7 rna polymerase gene is the lacuvs promoter, which is inducible by iptg
用iptg誘導表達t7rna聚合酶，以表達質粒上的目的基因。在葡萄糖存在的條件下，用常規方法發酵和誘導（ 37 1mmiptg ） ，發現表達的蛋白質條帶的分子量與理論上計算的分子量一致。但是發酵液中檢測不到吲哚，表明雖然表達了目標蛋白，但表達的蛋白質沒有酶活性。
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