maldi 中文意思是什麼

maldi 解釋
基質輔助雷射脫附游離
  1. For protein identification, proteins spots of interest on the gels stained with colloidal coomassie brilliant blue g - 250 were excised, digested in - gel with trypsin, and analyzed by peptide mass fingerprinting ( pmf ) with matrix assisted laser desorption / ionization - mass spectrometry ( maldi - ms ). erp60, trypsinogen, proelastase, lipaseandso on were identified. this will enable us to present an overview of the proteins expressed in rat pancreas tissues and lays the basis for subsequent comparative proteome analysis studies with pancreas development

    從中隨機選擇一些差異蛋白質點，進行基質輔助激光解吸-電離飛行時間質譜（ maldi - tof - ms ）測定其膠內酶解后的肽質指紋圖譜，用mascot軟體查詢swiss - port數據庫，初步鑒定為一些與生長發育、物質代謝、細胞因子、信號轉導等有關的蛋白質，如erp60 、 trypsinogen 、 proelastase 、 lipase等。
  2. Proteins were identified with peptide mass fingerprinting using matrix - assisted laser desorption ionization time of flight mass spectrometry ( maldi - tof ms ) after tryptic in - gel digestion

    差異蛋白經胰蛋白酶酶切后產生肽片段，再利用基質輔助激光解吸電離飛行時間質譜得到肽指紋圖譜來鑒定。
  3. Primary strucure and identification of a home - keeping protein ( a73 ) were obtained by maldi - tof / ms and databases. protein a73 is a dna repair protein, named recn, which is indispensable protein in genetic recombination and recombination repair

    （ 2 ）不同生長期的rt19的蛋白表達數量和種類約有80是相同的，證實rt19在正常的生理過程中大部分蛋白的表達是穩定的，即為持家蛋白（如a73 ） 。
  4. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  5. Identification of vimentin in subline coc1 ddp by maldi - tof mass peptide map analysis

    亞細胞中波形纖維蛋白的肽質譜指紋分析
  6. Analysis of the primary structure of rhepo by maldi - tof - ms

    一級結構的質譜分析
  7. Application of maldi - tof - ms in biochemistry and polymer chemistry

    在生物化學和高分子化學中的應用
  8. About 25 salt - induced proteins were identified by maldi - tof / ms, and finally the function of 17 induced proteins were determined, including heat - shock proteins ( groes, clp ). abc transporter, rna polymerase ( p - subunit ) and enzymes involved in signal transduction and metabolism. meanwhile, 84 proteins were idifferentially expressed after salt shock 5 min and 50 min in late exponential phase

    應用maldi - tof ms對30個鹽脅迫蛋白進行分析，已初步確定17個誘導蛋白的功能，其中包括與鹽脅迫相關的熱激蛋白（ groes 、 clp ） 、與代謝途徑和信號傳導等有關的酶，以及abc轉運蛋白、轉錄調節蛋白、 rna聚合酶的亞基等。
  9. 57 protein spots out of about 1000 detectable spots on the 2 - d gels were indentified by the following two methods : l ) n ~ terminal edman degradation microsequencing after the protein spots were electro - transferred to pvdf membrane. 2 ) maldi - tof - ms peptide fingerprint analysis of the protein spots and protein database searching. the 2d protein maps of the rice spikelet during the sterile and fertile stages were compared

    二是通過原位酶解，抽提蛋白質片段進行maldi - tof質譜分析，利用肽質量指紋圖數據在數據庫中進行檢索，在雙向電泳凝膠上取了57個點進行分析，有34個蛋白質點在數據庫得到歸屬鑒定。
  10. Recently the coupling of ims equipped with electrospray ( esi ) or matrix - assisted laser - desorption ( maldi ) ion sources to mass spectrometry ( ms ) has extend its application to the biomedical area

    近年來，離子遷移譜技術與電噴霧和基質輔助解吸附等離子化技術的結合，以及與質譜技術的聯用，使得該技術的應用迅速拓展到生物醫學領域。
  11. The center is equipped with state - of - the - art facilities, including the most modern two - dimensional gel electrophoresis systems, automatic gel excision and in - gel digestion systems, as well as q - tof and maldi - tof

    基因體研究核心設施之一的蛋白體學實驗室，裝備了最尖端的蛋白體
  12. Twenty - six induced proteins were analyzed by matrix assisted laser desorption / ionization time - of - flight mass spectrometry ( maldi - tof / ms ) and identified by database searching. among them, 20 ones were assigned and their functions were known, including salt stress proteins, or enzymes are related to salt stress, y - butyrobetaine and iipopolysaccharide synthesis, metabolism pathway and signal transduction

    應用maldi - tof ms對26個鹽激誘導蛋白進行分析，將獲得的肽指紋圖譜經數據庫檢索，初步確定了20個誘導蛋白的功能，其中包括與鹽脅迫、 -丁酰甜菜堿、脂多糖合成、代謝途徑和信號傳導等有關的酶。
  13. The maldi peaks corresponding to the c - terminal peptide fragments of proteins were specifically enhanced, discriminating against those from internal peptides that were not tagged with a positive charge

    與那些沒有正電荷標記的中間肽段的maldi譜圖峰明顯不同的是，蛋白質c端肽片段的maldi譜圖峰明顯增強。
  14. Fast detection of snps of human mtdna using primer extension - maldi - tof ms

    技術在農田土壤微生物多樣性研究中的應用
  15. Moreover, the taxol of zymolytic extraction is analyzed by thin layer chromatography ( tlc ), high performance liquid chromatography ( hplc ), micellar electrokinetic chromatography and maldi - tof

    並對發酵提取物內的紫杉醇用tlc 、 hplc 、毛細管電泳和maldi ? tof進行了定性定量分析。
  16. Methods and results : proteomics approaches involve three critical techniques : two dimensional electrophoresis, biological mass spectrometry and bioinformatics. in 2 - de, we applied traditional ief in the first dimension and tris - tricine system in the second dimension and acquired protein profiles of serum and skin samples. in the identification of 10 proteins of serum and 16 proteins of skin, we acquired peptide mass of fingerprint ( pmf ) maps for all targets by means of matrix - assisted laser desorption - ionization time of flight mass spectrometry ( maldi - tof - ms ) and partial aminio acid sequences for 9 proteins by means of electrospray ionization ms / ms ( lisi - ms / ms )

    研究方法：採用雙向電泳技術獲取泥鰍創傷前後血清和皮膚的小分子蛋白圖譜，利用基質輔助激光解析飛行時間檢測質譜分析（ matrix - assistedlaserdesorption - ionizationtimeofflightmassspectrometry ， maldi - tof - ms ）和電噴霧離子化串連質譜分析（ electrosprayionizationms / ms ， esi - ms hs ）分別獲得差異蛋白點的肽指紋圖譜（ peptidemassoffingerprint ， pmf ）和部分序列信息，通過網際網路上的expasy服務器中和ncbi的相關軟體將這些信息和swissprot數據庫進行匹配鑒定蛋白質種類。
  17. The enzyme is homologous dimmer with molecular mass 102600 as determined by gel filtration on hplc and subunit mass 52042 as determined by maldi - tof mass spectrometry. the optimal ph of d - hydantoinase is at near 9. 5 and the optimal temperature is at 45

    通過對其生化性質的研究表明，其單體m _ r為52042 ，天然m _ r為102600 ，最適ph為ph9 . 5 ，最適溫度為45 ，不同的二價金屬離子對酶活性有不同的影響。
  18. Monitoring reaction of polymer using maldi - tof

    監控高分子合成反應
  19. Therefore, using maldi, samples containing thousands of peptides can be compared in a minimal amount of time

    因此，使用maldi ，含有數以千計肽段的樣品可以在最短的時間內進行比較。
  20. Application of a conventional maldi matrix facilitated desorption and ionization of peptides and proteins for molecular weight determination of the separated compounds

    使用傳統的maldi基質利於肽段和蛋白質的解吸和離子化，從而可進行被分離分子的分子量測定。
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