male sterility gene 中文意思是什麼
male sterility gene
解釋
雄性不育基因-
Analysis of the fertilily restorer gene in the wild - abortive type cytoplasmic male sterility system with the issr and sslp markers
微衛星標記分析秈粳亞種間的遺傳多樣性 -
This review focuses on the progress of cms studies from three aspects : ( 1 ) cms of mitochondria through interference of the energy supply. ( 2 ) the effects of a fertility restorer gene, and ( 3 ) the application of cms in genetic engineering of plant male sterility
主要以能量為切入點闡述了植物線粒體引起細胞質雄性不育的作用機制，對提出的2種主要假說做了相關分析；從轉錄后調控和翻譯后調控2個層面對育性恢復的機製作了詳細的闡述；綜述了近年來細胞質不育在植物育種上的應用所取得的成就。 -
Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis
分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。 -
The technology and methods of the study on molecular mechanism of cytoplasm male sterility ( cms ) are introduced in regard to mitochondrial genome, mitochondrial gene, mitochondrial rna, mitochondrial protein, transformed plants and abortion of pollen
摘要本文從線粒體基因組、線粒體基因、線粒體轉錄rna 、線粒體蛋白、轉基因植物以及花粉敗育機理六個方面詳細介紹了植物細胞質雄性不育分子生物學研究的技術和方法。 -
It was reviewed here that the major advances of biological research on the fertility restoration gene for cytoplasmic male sterility in plant in the past decade
摘要綜述了近10年來植物細胞質雄性不育恢復基因分子生物學的研究進展。 -
Hsp70, a kind of molecular chaperone, has the main functions of taking part in protein folding, protein degredation and reparation of dna damadge and has important effects on the constructure and function of mitochondria. it has already been proved that there is a close correlation between hsp70 and the development of plants and animals. this paper deals with integrating sense and antisense cdnas of hsp70 into tobacco dna by constructing an expression vector of sense and antisense cdnas of hsp70 and gene - transforming methods - genegun bombarding and agrobacterium mediation. provided expression of hsp70 gene is inhibited by sense and antisense cdnas of hsp70 we can get male sterile plants so as to prove that antisense cdna of hsp70 leads to male sterility 1
Hsp70是一種分子伴侶，主要功能是參與細胞有關蛋白新生肽的折疊、亞基組裝、細胞內運輸、蛋白質降解及dna損傷的修復，對線粒體結構和功能發揮重要作用，已有研究證明hsp70與動植物的發育有密切的關系，本研究將hsp70正、反義cdna構建成表達載體，並運用基因槍和農桿菌介導法將hsp70正、反義cdna導入煙草，試通過hsp70反義cdna抑制hsp70基因的表達從而創造雄性不育株，以證明hsp70反義cdna能創造雄性不育系。
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