marker band 中文意思是什麼
marker band
解釋
指示薄層-
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No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。 -
9 expression of mxmybl gene in e. coli was experimented. the structure of its recombinant expression vectors had not base mutant and drift. sds - page of pet - mxmybl proteins showed that there was a strengthened protein band in expectant 38kda protein marker
構建的原核表達載體結構正確，未出現堿基突變及移碼現象； lmmoffeiptg誘導zh后，在預期的蛋白分子量約38kda處出現一條表達加強的蛋白帶。
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