marker enzyme 中文意思是什麼
marker enzyme
解釋
標志酶-
Application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats to screen the exact, dependable, particular genetic monitoring marker method of laboratory animal, the author had studied the application of microsatellite dna polymorphisms and dna fingerprints to inbred strain mice and rats, and compared the two methods with the biochemical marker enzyme method. the study had established the foundation of the molecular genetic monitoring marker method of laboratory animal
本文通過對dna指紋技術和pcr擴增微衛星dna技術在近交系大、小鼠遺傳檢測中的應用研究，並與生化位點標記分析法進行比較，旨在篩選出具有精確、可靠、特異性好的實驗動物遺傳檢測方法，為建立分子生物學實驗動物遺傳質量監測技術和標準奠定基礎。 -
It has made the strong basis for further studying mechanism of replication, virulence and determinant, attenuation, pathogenesis, functions of genetic products, specific diagnosis, cell and host tropism, development of dna vaccine and marker vaccine of csfv, and provided an excellent tool for molecular virology. main research contents include : based on published nucleotide sequences of csfv and by the help of computer analysis software, high conservative regions and single restriction enzyme sites of genome were selected. utilizing rt - pcr and nested - pcr techniques, 7 overlapping cdna fragments covering the full genome of csfv c - strain were successfully amplified
中國豬瘟兔化毒（脾淋毒）基因組cdna文庫的構建、序列分析：根據已發表的豬瘟病毒（ csfv ）核苷酸序列，藉助計算機軟體分析，選擇高保守區段和基因組中的單一限制性酶切位點，利用rt - pcr及nested - pcr和helf - nestedpcr技術，成功地擴增出了覆蓋c -株全基因組的7個cdna重疊片段f1 f7 ，分別克隆到pmd - 18t或pgem - teasy載體進行測序后，拼接出了其核苷酸序列。 -
Dna fingerprints of 13 colonies of 5 strains, including 5 balb / c, 2 balb / c - nu / nu > 4 c51 1 cba / n and 2 dba / 2 from 7 factories of laboratory animal in the beijing and xi ' an areas, were studied with jl - 2 mulilocus probe and southern hybridization and the author compared the dna fingerprint with the biochemical marker enzyme method. it indicated that the fingerprint had 17 - 22 distinguishable fragments and these fragments had high polymorphisms. the fingerprints of balb / c and balb / c - nu / nu, which were abnormal at the hbb site with the biochemical marker enzyme method, was different from their normal groups
實驗採用jl - 02多位點探針對北京和西安地區較大的7家實驗動物生產供應單位的5個balb c群， 2個balb c - nu nu群， 4個c _ （ 57 ）群， 1個cba n群和1個dba 2群近交系小鼠進行了dna指文圖分析，並與常規生化標記分析法進行了比較，結果顯示：所產生的dna指紋圖的圖帶數均在17 - 22條，具有良好的多態性。 -
The main identifying techniques including morphological marker, pollen morphology, iso - enzyme and dna molecular marker which have been applied to tomato species classification and cultivar identification are introduced
綜述了形態學標記、花粉學方法、同工酶標記和dna分子標記等分類方法在番茄種質資源分類和品種鑒定上的應用及研究進展。
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