marker gene 中文意思是什麼
marker gene
解釋
標志標記基因-
A pcr - based dna marker for a - gliadin gene in common wheat
醇溶蛋白基因的分子標記 -
Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker
本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。 -
Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions
本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。 -
Transgenic plants and their marker gene knock - out
轉基因農作物及其標記基因的消除 -
We designed one primer pairs ctb - 1, ctb - 2 to amplify about 580bp of cyt b gene sequence as a molecular marker to analyze phylogenetic relationship of 14 species of oedipodidae in china. dna sequences were aligned using clustal x, followed by refinement by eye based on the corresponding deduced amino acid sequences. after cutting off 5 " and 3 " termini unaligned sequences, we get 462 bp segment
本研究從分子生物學角度入手，採用cytb作為分子標記，採用自行設計的一對cytb基因特異引物ctb - 1 、 ctb - 2 ，通過pcr技術，共獲得斑翅蝗科4個亞科14個種的代表個體以及癩蝗科1個種的代表個體的580bp左右的cytb部分序列。 -
We discussed the advances of research on resistant breeding for frogeye leaf spot of soybean from three aspects, resistant breeding, resistant germplasm screening and marker on resistant gene for frogeye leaf spot, which could provide information for resistant breeding
摘要從抗灰斑病育種、抗性資源篩選和抗灰斑病基因分子標記三個方面闡述了國內外抗大豆灰斑病育種的研究進展，為抗灰斑病育種者提供可參考的相關信息。 -
Sinense y. x. lin using allzyome marker. the experiment employ six enzymes : est, mdh, fdh, gdh, sod and acp, using their polymtic locus to analyse the allele gene frequency, the number of the effective allele gene
採用est 、 mdh 、 fdh 、 sod和acp等5種酶系統，分析荷葉鐵線蕨的等位酶遺傳變異，用間接法估算荷葉鐵線蕨交配系統的特徵參數? ?異交率t 。 -
The isozyme took one kind of important genetic marker is widely applied to biology research each domain, the plant isozyme can in the very great degree be able to reflect between the plant individual the heredity difference, is surveys the gene difference and the hereditary change one important method
同工酶作為一種重要的遺傳標記被廣泛應用於生物學研究的各個領域，植物同工酶能夠在很大程度上能反映植物個體之間的遺傳差異，是探測基因差異和遺傳變異的一種重要手段。 -
Lea3 gene probe and marker probe were labeled by dig - chem - link
Okblea3片段，並用地高辛標記法進行了lea3基因探針的制備。 -
Identification and marker - assisted selection of hmw - glutenin 1dx5 gene in wheat
技術研究中國特有小麥種子貯藏蛋白基因的遺傳變異 -
Three chloroplast transformation vectors including pds16s - cat, ptn1269 - bar and psp72 - n5 - bar - n3 were constructed, using ! 6s rrna or chln gene sequence as a homologous segment and cat or bar as a selective marker gene, respectively. foreign genes were introduced to the cells of d. salina by microprojectile bombardment method and a pilot chloroplast tran
3 .杜氏鹽藻葉綠體165出na基因的克隆和轉化載體的構建根據杜氏鹽藻的近緣藻類的葉綠體基因組序列資料，克隆了杜氏鹽藻葉綠體16srrna基因部分序列1100bp ，並利用克隆的16srrna鄭州大學2003年博士學位論文 -
( 2 ) the interest gene in pcar is under the control of acti which is the strong promoter in rice. the selectable marker gene is hyg gene
（ 2 ）表達載體pcar中目的基因由單子葉強啟動子水稻act1啟動子調控，選擇標記基因為潮黴素抗性基因。 -
The ms188 gene was finely mapped. a total of 8 new indel markers were designed to map msl88 using a segregating population with a total of 2135 male sterile progenies. ms188 was finally mapped to a region of 95. 8kb between the molecular marker mda7 and k24c1
在與ms188連鎖的分子標記mc015附近設計了8個indel分子標記，對遺傳群體中2135株不育植株進行基因型分析，最後將目的基因定位於第五條染色體分子標記mda7和k24c1之間95 . 8kb的區間內。 -
Construction of male sterility expression vector by integration of artificial sense and antisense cdnas of hsp70 into puc18 and puc19 respectively, we can obtain psc and pac. tapertal specific expression promoter ta29 and terminator nos are connected directionally with sense and antisense cdnas of hsp70 extrected and purified from psc and pac., then integrated into puc18 and puc19, by which we can build sense and antisense cdna nos ( respectively named plasmid 650 and plasmid 651 ) of ta29 - hsp70. for the sake of better screening and examination of transformed gene, we cut plasmid 650, plasmid 651 and 3301 ( containing gusgene bar screening marker gene ) with hindiii and ecor i enzymes, then connect purified fragments of 650and 651 with plasmid 3301 to construct the vector 3301 + 650 and 3301 + 651. corroboration of whether sense and antisense cdna - nos is integrated into plasmid3301 can be made by plate screening and enzye - cutting analysis
分別將從psc 、 pac回收純化的hsp70正、反義cdna與絨氈層特異表達啟動子ta29及nos終止子定向連接，然後插入到puc18 、 puc19中，構建成花藥特異表達的ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos ，分別稱作650和651質粒。為了更好地對轉化子進行篩選和檢測，用hind和ecor分別對650 、 651及3301質粒（含gus報告基因和bar篩選標記基因）進行酶切，將從650和651回收純化的目的片段與3301質粒進行連接，再對重組子進行平板篩選和酶切分析確定ta29 - hsp70sensecdna - nos和ta29 - hsp70antisensecdna - nos插入到3301質粒中，構建成3301 + 650和3301 + 651表達載體。 -
Linkage analysis plays an important role in gene mapping. the foundation : the two gene locuses which locate on the same chromosomal ( eg. disease gene and marker gene ) happen to cross over and recombine. the farther the distance between two locuses is, the higher the probability happening to cross over is, the lower the probability that the two locuses are inherited to offspring together is, that is, the degree of linkage is not strong. so we can estimate the distance and the degree of linkage by the recombination fraction between the two locuses to locate gene
連鎖分析是基因定位主要策略之一，其基本原理是位於同一染色體上兩個基因位點（例致病基因與標記基因）在減數分裂的過程中會發生交換與重組，染色體上的兩個位點間距離越遠，發生重組的概率就越大，兩個位點在一起傳給後代的機會就越少，即連鎖程度弱，這樣由標記位點與疾病位點間的重組率可估算出兩者間的距離以及連鎖程度，達到基因定位的目的。 -
( 3 ) the interest gene in p3301rip is under the control of double 35s promoter and the selectable marker gene is bar gene
（ 3 ）表達載體p3301rip中目的基因由雙35s啟動子調控，選擇標記基因為bar基因。 -
Bar gene also is one of widely used selective marker gene. the cloning of bar gene is important to plant transgenic engineering, gene expression and studying physiology
而且bar基因也是迄今為止應用最為廣泛的一個抗除草劑選擇標記基因，克隆出bar基因對植物的遺傳轉化，基因表達和生理學研究都有重要的作用。 -
2. an anther specific chimaeric male sterile gene expression box with a enhanced promoter ( ta29 ) driving coda gene was constructed and the expression box was inserted into binary vector p3301 that contains a l - phosphinothricin ( ppt ) - resistant selective marker gene and - glucuronidase ( gus ) reporter gene in t - dna region
以增強的ta29啟動子驅動克隆的coda基因，構建成花藥特異性嵌合基因表達盒；將此表達盒插入雙元載體p3301 ，構建成以ppt抗性基因為選擇標記，以gus為報告基因的植物表達載體。 -
Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced
在一步轉化系統中，光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求，並且簡化、優化了整個共培養過程，省去了一篩、二篩和預分化步驟，只用7周的時間就可以初步得到再生轉化植株；共191塊愈傷組織得到125塊抗性愈傷組織，轉化頻率達到65 . 4 ，最後共得到99棵獨立來源的再生植株，抗性愈傷組織再生頻率達到79 . 2 。 -
Consequently the desirable gene may be linked to a marker gene, e. g. a gene conferring resistance to an antibiotic in the growth medium
如決定一種是否對培養基上抗菌素產生抗性的性狀基因，就可作為標記基因用來判斷外源基因是否轉入。
分享友人