mature peptide 中文意思是什麼
mature peptide
解釋
成熟肽-
Hovever, the gene encoding the mature papain peptide was amplified using pcr from genomic dna extracted mid - development carica papaya fruit. about 98. 8 % of cdna nucleotide sequence reported in literature were homologous to the responding sequences of our study. there are three introns in the gene, in which the content of a and t is 86. 0 %, 79. 5 % and 90. 6 % respectively
同時本研究以木瓜基因組dna為模板，通過pcr反應獲得了編碼木瓜蛋白酶成熟多肽部分的核苷酸序列，序列分析表明該基因含有三個內含子，其長度分別為157bp 、 266bp 、 88bp ， at含量分別為86 . 0 ， 79 . 5 、 90 . 6 。 -
The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank
分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。 -
The - glc squence of tea plant consists of 1475 bp, including 189bp 3 ' utr, 233bp 5 ' utr and 1050bp open reading frame. the deduced amino acids ( aa ) sequence is a 350aa - glc precursor which consists of an 82aa transit peptide and a 257aa mature protein
結果表明，該序列的3 』 -端和5 』端的非編碼序列長度分別為189bp和233bp ，開放閱讀框為1050bp ，編碼350氨基酸，其轉運肽長度為82個氨基酸，成熟蛋白由257個氨基酸組成。 -
Construction of recombinant pichia pastoris containing cdna encoding mature peptide of human bone morphgenetic protein - 7 bmp
7成熟肽畢赤酵母表達重組子的構建 -
Expression of gene coding mature peptide of sag1 of toxoplasma gondii in yeast pichia methanolica
1成熟蛋白編碼區基因在甲醇酵母中的初步表達 -
Large scale fermentation, expression and purification of recombinant human bone morphogenetic protein 4 mature peptide from escherichia coli
基因重組人骨形成蛋白4成熟肽在大腸桿菌中的大規模發酵表達及純化 -
In this dissertation, the following experiments are conducted : firstly, cloning of hpab - gene, i ) the amino acids sequences of 23 animal - peptide antibiotics were analyzed by multiple sequences alignment method and a pair of degenerated primers were designed according to the consensus sequence derived from mature peptides of some beta peptide antibiotics
人源肽抗生素hpab -的基因克隆：對動物23種型肽抗生素的氨基酸序列進行對比，選擇部分相似性較高的序列，分析其成熟肽的結構特點，抽提型肽抗生素的一級結構特徵，並以此為基礎設計簡並引物。 -
Expression of mature peptide of human bone morphogenetic protein - 7 in escherichia coli
7成熟肽在大腸桿菌中的高效表達 -
The cdna sequence with 789 bp encodes an uncompleted open reading frame ( orf ) of 263 amino acids, which included a signal peptide of 18 amino acids, a proposed propeptide of 171 amino acids, a partial mature protein of 74 amino acids. the sequences of signal peptide and propeptide are very similar to ones from wannan agkistrodon acutus reported by fan chunyan, but it only contains partial amino acids of mature protein. the 800 bp is likely to be a part of gene encoding metalloproteinase agkistrodon acutus from guangxi province
結論： 600bp產物和已報道的皖南五步蛇纖溶酶金屬蛋白酶氨基酸序列相比較，有90 . 6 %的同源性； 800bp產物中信號肽及前肽和已報道的皖南五步蛇金屬蛋白酶很相似，同源性為97 . 9 % ，但僅含有部分金屬蛋白酶成熟肽的氨基酸序列，推測此片段為廣< wp = 6 >西五步蛇金屬蛋白酶原基因的一部分。 -
Based on the high homologous n - terminal amino acid sequence between ba - dfe and subtilisin bpn, the fragment encoding ba - dfe mature peptide was amplified from the total dna of b. amyloliquefaciens dc - 4 by pcr
根據ba - dfe的n -端序列與subtilisinbpn高度同源設計引物， pcr擴增出ba - dfe成熟肽編碼區片段。 -
Due to high homology of nucleotide between ba - dfe and subtilisin bpn, primers were designed and synthesized. the intact ba - dfe gene was amplified by pcr and cloned. the sequence analysis indicated that the ba - dfe gene has an open reading frame with 1146bp, which encodes 382 amino acid residues containing signal peptide, pro - peptide and mature peptide
序列分析顯示，該片段的核昔酸序列中含有1146hp的開放閱讀框，可編碼382個氨基酸殘基的ba dfe前體蛋白，包括30個氨基酸殘基組成的信號肽、 77個氨基酸組成的前導肽和275個氨基酸殘基組成的成熟肽。 -
The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr. sequence analysis of the gene was described in this papel the amino acid sequence, as derived from the nucleotide sequence of a cdna clone, had high homology with other subtilisin - like serine protease of nematogenous fimgi
與其他絲氨酸蛋白酶基因序列比較表明，與其他線蟲卵寄生性真菌如paecilomyceslilacinus 、 verticilliumchlamydosporium及m . anisopliaevar . anisopliae同源性較高，而與捕食線蟲真菌arthrobotrysoligospora同源性較低（ 45 ） 。 -
Secondly, determination of the antimicrobial activities of synthetic hpab -. i ) the mature peptide of hpab was synthesized by fomc method and purified by reverse phase hplc. the molecular weight of synthesis peptide was 4334. 96da characterized by mass spectrometry, highly agreed with the theoretical mw of hpab - 3
2 . hpab一p抗菌活性初步鑒定:採用固相法合成了hpab一p成熟膚，經即一hplc純化後用質譜測定合成膚的分子量為4334 . % da ，與其理論分子量相符。 -
Genes coding mature peptide of igfs were achieved by pcr using another pair of oligo - nucleotide primers to induce to the suitable restriction enzyme site, and the igf - i product of pcr contains 230 base pairs. igf - ii contains 219 base pairs. 3
各另外設計一對特異性pcr引物，導入適當限制性內切酶切點，以上述連有目的基因的克隆載體為模板，採用pcr方法擴增基因片段，獲得長度約230bp的igf -和219bp的igf -成熟肽基因序列。 -
The cdna contains 947 nucleotides that codes for a precursor protein of 216 amino acids. sequence analysis shows that the n - terminal 1 - 25 amino acid sequence is a predicted signal peptide and other 26 - 216 amino acid sequence is a mature peptide which has no transmembrane domain
Ecbp21cdna全長947bp ，編碼216個氨基酸，序列分析顯示其n端1 - 25氨基酸為信號肽， 26 - 216氨基酸為成熟蛋白肽段，並且不具有跨膜結構域。 -
Secreted expression of mature peptide gene of human bone morphogenic protein - 7 in pichia pastoris
7成熟肽基因在巴斯德畢氏酵母中的分泌表達 -
Ba - dfe mature peptide - coding sequence was cloned to the restriction site ncol and ndei of the expression vector pet - 15b, respectively, and highly expressed in e. coli bl21 ( de3 ) by the form of non - fusion and his - badfe fusion protein
Lmmol liptg誘導時，融合表達時產生分子量約為30kd 、帶有his標簽的ba dfe融合蛋白，非融合表達時則產生分子量為28kd的ba dfe 。 -
The cleavage site of putative signal peptide was predicted to occur between 28 ( ala ) and 29 ( ala ) thus the putative mature form of the protein composed of 92 amino acids with a molecular weight of 9. 2 kda
推測的最可能的信號肽切割位點位於第28 （ alal和29 （ alal個氨基酸之間。椎測的成熟肽共有92個氨基酸，分子量9 zkda 。 -
Armigera was analysed and then registered in genbank and accession number was af515667. the cdna encodes a protein with a plltative 2l amino - acid signal peptide and a 425 amino - acid mature protein with molecular mass of 50 kda
連接產物轉化jm ，提取質粒進行酶切及pcr分析，將此連接產物命名為pchto將pcht轉化blzi （ de3 ） ，經iptg誘導，提取菌體內容物sds page電泳。 -
The result of sequencing analysis shows that the coding region of the ba - dfe mature peptide has 825bp in length and encodes 275 amino acid residues, which has a n - terminal amino acid sequence identical to that of the purified ba - dfe determined by edman method, indicating that the cloned fragment was indeed as expected
測序結果發現，其成熟肽編碼區有825bp ，編碼275個氨基酸殘基，推導的n -端序列與測得的該酶n -端序列完全一致，證明了m川人學博：學位論文該片段確實是ba dfe成熟肽編碼區。
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