molecular clone 中文意思是什麼

molecular clone 解釋
分子克隆
  • molecular : adj. 分子的,由分子形成的,分子內[間]的。adv. -ly
  • clone : n. 1. 【生物學】純種細胞，無性系。2. 復製品；（幾乎）一模一樣的人。3. （不動腦筋）機械行事的人；機器人。vt. 把…培養為純種細胞；無性繁殖；〈比喻〉復制。
  1. It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro. thus, we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag, designated as eiav - pok8. 2 - his

    本研究應用已構建好的eiav驢白細胞弱毒疫苗株的感染性分子克隆載體（ pok8266 ）為模板，通過soepcr方法在感染性分子克隆載體的s2基因獨特區內引入突變點，形成含有酶切位點（ nspv ）的突變體（ p1p4 ） 。
  2. Full - length clone of protein ht036 gene interacting with endostatin and its molecular mechanism in promoting the apoptosis of vascular endothelial cell

    036基因的全長克隆及其促血管上皮細胞凋亡的分子機制
  3. It is very important to investigate and clone related genes under fe - deficiency stress in malus xiaojinensis, which not only provides the probability for exploring molecular mechanism of the absorbability and transport of fe, but also may enhance the resistance under fe - deficiency stress of plants by inserting related genes into chromosome genome

    因而研究和克隆缺鐵脅迫相關基因不僅為進一步研究蘋果吸收、轉運鐵的分子機制提供基礎。其次，在提高植物對鐵脅迫抗性的遺傳育種中，可以利用基因工程技術，將鐵脅迫相關基因整合到作物染色體基因組中，從而達到有效控制鐵營養的目的。
  4. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  5. Sequence analysis the cdna clone contains an 546bp orf ( open reading fragment ) encoding a predicted protein of 181 amino acids with molecular weight of 20. 7kd, 130bp 5 ' non - coding region and 152bp 3 " non - coding region including polyadenylation signal sequence aattaa and poly a tail. 3

    棉花arf基因的序列分析核苷酸序列分析表明， gharf的全長為828bp ，其中含有一個從131bp到676bp間的546bp構成的一個完整開放閱讀框，它編碼181個氨基酸、分子量為20 . 7kd的蛋白質。
  6. Immunological characteristics of equine infectious anemia virus derived from infectious molecular clone

    感染性分子克隆衍生的馬傳染性貧血病毒的免疫學特性
  7. The cdna expression library that contains no intron theoretically include all expressed genes and conserve resource genes permanently. lt can be used to find out and clone some genes which can express particular protein with modern molecular biological techniques, such as immunological screening, drug - prob screening, southern et al. lt is very important to study the life nature of plasmodium falciparum in molecular level. with the developments of these studies, the drug - resistant mechanism of the plasmodium falciparum and the genes of some specific medicine binding protein can be made well - known. at the same time, the researches will do good to explain the mechanism of some specific medicines in order to design and screen new anti - malaria drugs

    建立cdna表達文庫在一次永久保存基因資源的同時，可以利用功能篩選、免疫學篩選、藥物探針篩選、 southern雜交和大規模序列測定等現代分子生物學技術尋找特異性活性蛋白基因，進而克隆和表達這些基因，對從核酸及蛋白質等分子水平研究瘧原蟲的生命活動規律，對揭示其抗藥性分子機理，搞清某些特效藥物結合蛋白的基因及此類藥物的作用機制，對新型抗瘧藥物的合理設計及篩選都具有極其重要的現實意義。
  8. The sense clone was induced with iptg. the expression of mstnd peptide was observed on sds - page. the results lay a good foundation in the application for molecular biological technique in animal nutrition

    經sds page電泳檢測表明，構建的重組菌能夠高效地表達豬肌生成抑制素c端80氨基酸編碼序列蛋白，其表達率在42左右。
  9. This result indicated that the virus pd70344v derived from the infectious molecular clone is a highly virulent strain which had inherited main characters from its parental virus dv

    這一結果說明本文所構建的感染性分子克隆株pd70344v具有其親本dv株的基本生物學性狀，是一株具有高致病力的衍生病毒。
  10. And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8

    試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。
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