molecular fragment 中文意思是什麼
molecular fragment
解釋
分子碎片-
A free radical is a molecular fragment having an unpaired electron.
自由基是一種具有未配對電子的分子碎體。 -
Molecular cloning and sequence analysis of lycopene - cyclase gene fragment from loquat
基因的克隆及序列分析 -
High stability of ptr102 - derived marking plasmids indicated that novel vector systems based on ptr102 could be constructed for the study on molecular biology and ecology of m. huakuii. 1kb gfp cdna fragment amplified by pcr was cloned into e. coli expression plasmid pet - hc, which was under the control of rna polymerase gene promoter from phage t7 with its own translation initiation codon
將pcr擴增的1kbgfpcdna片段克隆到大腸桿菌表達載體pet - 11c上，使gfpcdna在帶有lac操縱基因的t7噬菌體rna聚合酶基因啟動子的控制下、以自身的atg作為翻譯起始密碼進行翻譯。 -
Some of the molecular ions fragment into smaller daughter ions and neutral fragments.
有些分子離子斷裂成為較小的子離子和中性碎片。 -
In order to investigate the genomic organization of the single - nucleocapid nucleopolyhedrovirus of helicoverpa armigera, the ecori - n fragment located at 54. 8 - 59. 3 kbp of the viral genome was sequenced. the fragment contained 3762 bp helicase gene potentially encoding a protein with a molecular mass of 146 kda
對棉鈴蟲單核衣殼核多角體病毒（ helicoverpaarmigerdsingle - nucleocapsidnucleopolyhedrovirus ， hasnpv ）基因組中ecori ? n片段進行序列分析，獲得了完整的解螺旋酶基因（ hel ） ，其開放閱讀框大小為3762bp ，編碼一個分子量為146kda的蛋白質。 -
Quantitative structure - activity relationship is a method building a statistical model. the model can quantificationally predict structure - activity relationship of molecule, and bioactivity of new molecules can be known. structure of molecule, which is described by parameters in physical chemistry, biochemistry and quantum chemistry, includes functional group, minor structure, molecular fragment, chemical composition
本文提出了一種定量構效關系的處理方法，即應用支持向量回歸方法解決定量構效關系，並且應用這種方法預測了芳香烴化合物生物降解度與喜樹堿化合物抗腫瘤生物活性，取得了比較令人滿意的結果。 -
In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein
經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。 -
The isospin effect and k production in intermediate and high energy heavy ion collisions ( hics ) are hot topics in the nuclear physics. based on the isospin - dependent quantum molecular dyanmics ( iqmd ) model and self - consistent relativistic boltzmann - uehling - uhlenbeck ( rbuu ) model, we have studied them and obtained some interesting results. as for the study of isospin in intermediate energy hics, we ' ve investigated how both stength ( q ) and density dependence of symmetry potential ( sp ) affect many measurable observables, such as the yield, phase - space, and isospin distributions of fragments, as well as the correlations between intermediate - mass - fragment ( imf ) multiplicity n and charged - particle multiplicity n, light - charged - particle ( lcp ) multiplicity n, and neutron multiplicity n,
在中能重離子碰撞的同位旋研究方面，分別研究了對稱勢的強度（ c _ s ）和其密度依賴形式對中能重離子核反應各類碎片產物產額、相空間、及其同位旋的分佈，中等質量碎片多重數（ n _ （ imf ） ）與帶電粒子多重數（ n _ c ） 、輕帶電粒子多重數（ n _ k ） 、中子多重數（ n _ n ）的關聯等多種實驗觀測量的影響，以獲取對稱勢中該兩方面的信息，尤其著重於研究如何分別獲取有關該兩方面的信息的途徑。 -
The dissertation consists of five chapters : in chapter one, the recent progress in molecular approaches in systematic studies of macroalgae e. g. dna extraction, restriction endonuclease fragment length polymorphisms ( rflps ), random amplified polymorphic dna ( rapd ), gene sequencing, intersimple sequence jepeats ( issr ), amplified fragment length polymorphisms ( aflp ) and single strand. conformation polymorphisms ( sscp ) were reviewed
本論文由五部分組成：在第一部分，綜述了大型海藻dna的提取、限制性片段長度多態性（ rflps ） 、隨機擴增多態性dna （ rapd ） 、核酸序列分析、擴增片段長度多態（ aflp ） 、單鏈構象多態（ sscp ）等分子手段在大型海藻系統學研究中應用的一些進展。 -
The identification of the phenylethanoid glycosides in plant extract of cistanche deserticola was based on the comparison of molecular ions, and the fragment ions obtained by ms ( superscript n ) experiments with those of the authentic standards and the data was reported in the literatures
通過得到的分子量和碎片信息並與標準化合物以及文獻中的已知化合物比較，建立了這一類化合物快速鑒定的質譜新方法。 -
One solobp ecor i fragment containing phospholipase gene was isolated. further sequence analysis and subcloning revealed a 963bp phla. gene coding a 320aa phospholipase phl with deduced molecular weight of 33kd
通過測序及亞克隆分析，發現一個磷酯酶的基因phla ，長度為963bp ，預測編碼一個由320個氨基酸組成，分子量為33kd的磷酯酶phl 。 -
Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected
Rapd （隨機擴增多態性dna ）技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術，它建立於聚合酶鏈式反應（ pcr ）技術基礎之上，利用隨機合成的寡聚核苷酸序列為引物（一般為10個bp ） ，分別與dna的兩條單鏈結合，在dna聚合酶的作用下，對基因組的特定區域進行pcr擴增，其電泳結果為不同大小和數目的dna譜帶即rapd圖譜，可反映基因組相應區域的dna多態性。 -
Sequence analysis the cdna clone contains an 546bp orf ( open reading fragment ) encoding a predicted protein of 181 amino acids with molecular weight of 20. 7kd, 130bp 5 ' non - coding region and 152bp 3 " non - coding region including polyadenylation signal sequence aattaa and poly a tail. 3
棉花arf基因的序列分析核苷酸序列分析表明， gharf的全長為828bp ，其中含有一個從131bp到676bp間的546bp構成的一個完整開放閱讀框，它編碼181個氨基酸、分子量為20 . 7kd的蛋白質。 -
Phaa, phab and phac were amplifed from the subclone of pseudomanas sp. producing pha by pcr. the gel electrophoresis analysis showed that the molecular weights of cloned phaa, phab and phac were equal to fragment speculated from three orfs
利用所提取的開放閱讀框架的序列設計三對引物，採用pcr技術，從合成pha的亞克隆片段中分離出phaa 、 phab和phac三個基因片段，經凝膠電泳分析表明，所克隆的三個基因分子量大小與推測的三個開放閱讀框架中基因片段大小一致。 -
After enzyme restriction and sequencing analysis, the nucleotide data had been further analyzed by antheprot 5. 0 and clutalw softwares. the analysis results showed that the cloned dna fragment had a longest open reading frame ( orf ) of 1035nt, it predicted to be encoded a 344 - aa protein with the molecular weight of 36kda
應用antheprot5 . 0 、 clustalw等分子生物學軟體分析，顯示主要外膜蛋白前24個氨基酸是較強的疏水性區域，可組成信號肽，其與omp基因的同源率達96 ，氨基酸的同源率高達98 。 -
Over 13 kb saci restriction fragment was cloned into pgem - 7zf ( + ), mapped for restriction endonuclease sites and an about 5. 0kb fragment was further subcloned and sequenced. through coding region specific primer, we amplied it ' s corresponding cdna, named st901. st901 is 2889bp long, contains 1447bp putative promoter region within 5 " upstream and three exons ( 475bp, 140bp, 39bp ) and two introns ( 472bp, 2s3bp ) in the coding region, encodes a hydrophilic protein of 217 amino acid residues with a molecular mass of 24kda
以同源探針篩選馬鈴薯基因組文庫，得到四倍體馬鈴薯基因組dna ? st901 ，基因全長2889bp ，含3個外顯子（長度分別為475bp ， 140bp ， 39bp ）和2個內含子（長度分別為472bp ， 253bp ） ； 5 』端含有1447bp的啟動子區段，該區段具備一般啟動子的基本元件tatabox和caatbox ； 3 』非編碼區長63bp ，具hind酶切位點，沒有發現保守的加尾信號。 -
In order to get small molecular g - protein rab3a, which serves to further investigate the interaction between rab3a and other proteins, we amplified the full coding region of rab3a cdna by polymerase chain reaction, using human placenta total cdna as template. the pcr products were recovered from gel electrophoresis and cloned into bamhi xhoi site of vector pyestrp2. the result of sequencing indicated that rab3a insertion fragment included its initiation and termination codons in 5 - and 3 - terminal, respectively
為了獲取全長的小分子g -蛋白rab3a ，以用於研究rab3a與其他蛋白相互作用關系，本實驗以人胎盤總cdna為模板， pcr擴增到人rab3a cdna全編碼區。產物回收后克隆于質粒pyestrp2的bamhi xhoi位點，測序結果表明，本實驗獲得的rab3a cdna包含了起始和終止密碼子。 -
And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。 -
In addition, molecular experiments indicated that resistance was not relevant to copy number or to insertion sites, but instead to the repeated gfp structure and to methylation of genomic dna. pvx vectors were constructed that contained the s6 gene of rbsdv vi and the 14 kd fragment of bnyvv rna2
分子檢測結果顯示， gfp3植株中普遍存在gfp基因的沉默現象，且gfpdna的甲基化程度明顯高於gfp1植株，說明這些植株對于病毒的抗性與目的基因的插入位點及拷貝數和無關，但與串聯重復的gfp基因結構形式密切相關。 -
The purified production was cloned into pmd18 - t vector. the cloned plasmids were transformed into jm109. the specific recombinant plasimid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resultant construct contained the gene of ibdv a fragment at right orientation
經瓊脂糖電泳檢測，將大小與預計分子量一致的片段純化后連接到pmd18 - t載體中，再轉化到jm109感受態細胞，得到的轉化子經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆，結果表明，得到的陽性重組子中含有a節段全長基因，插入到載體中的方向正確。
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