monoclonal antibody 中文意思是什麼
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With the polyclonal antiserum against testin and actn1, and the monoclonal antibody against ap - 2a, the expression of testin, actn1 and ap - 2a in hela cell was determined
用制備所得的抗gst - testin血清和抗gst - actn血清及ap - 2的單克隆抗體分別檢測了這三種蛋白質在hela細胞中的表達。 -
Estriol ; cell fusion ; monoclonal antibody
雌三醇細胞融合單克隆抗體 -
All samples of the tumor tissues were observed by electron microscope. the t lymphocyte subsets and b lymphocytes were detected and counted with monoclonal antibody technique before and after the cryotherapy. results : the cured percentage of internal hemorrhoid, mixed hemorrhoid and chronic fissura ani reached 83. 5 %, 66. 8 % and 62. 4 % respectively. the clearance rate of the verruca acuminata was 100 %
方法：取自西京醫院15年間冷凍治療肛腸區良性病變7018例，惡性病變636例，冷凍前後經電鏡觀察癌細胞變化及應用單克隆技術檢測患者t細胞亞群、 b細胞水平。 -
Production and characterization of anti - estriol monoclonal antibody
雌三醇單克隆抗體的制備與表徵 -
Effect of immunotherapy with the monoclonal antibody oc859 on survival rate and immunoreaction of patients with ovarian cancer
859治療對卵巢癌患者生存率及免疫系統的影響 -
The studies provided fundamental data and materials not only for the development of monoclonal antibody ( mab ), but also for the preparation and development of special diagnosis kits of h5 aiv subtypes
結果表明，該融合蛋白能同h5亞型陽性血清發生特異性結合，並且同h7 、 h9亞型aiv陽性血清沒有交叉反應。 -
Preparation and characterization of monoclonal antibody against botulinum neurotoxin type b
型肉毒毒素單克隆抗體雜交瘤株的建立 -
By sds - page and immuno - blotting, we found that a monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain antibody could react with cytoplasmic dynein intermediate chain - like protein at 67 kda in lily pollen. under confocal laser scanning microscopy after immunoflurescence labeling, we found that the dynein intermediate chain - like protein appeared punctated and was co - localization partly with microtubules in cytoplasm of lily pollen tube
免疫熒光標記及激光共聚焦掃描顯微鏡觀察發現，類細胞質力蛋白中間鏈在百合花粉管中存在於顆粒狀細胞器上；免疫熒光雙標及激光共聚焦掃描顯微鏡觀察發現，百合花粉管中類細胞質力蛋白中間鏈和微管存在部分共分佈。 -
Frankfurt os, robb ja, sugar baker ev, et al. monoclonal antibody tosingle2stranded dna is specific and sensitive cellular marker of apoptosis [ j. exp cell res, 1996, 226 ( 2 ) : 3872397
吳國慶，李少華，徐志偉，等.抗單鏈單克隆抗體雜交瘤細胞株的建立及鑒定[ j ] .細胞與分子免疫學雜志， 2002 ， 18 （ 5 ） : 4792480 -
Aeromonas hydrophila : developing its monoclonal antibody against aerolysin and preliminary application
嗜水氣單胞菌氣溶素單克隆抗體的制備及初步應用 -
The two cdna fragments, ap - 2a full length cdna and ap - 2 a cdna fragments were inserted in frame into gst gene fusion system. with an ap - 2a monoclonal antibody we detected the expression of gst - ap - 2a
將這兩段篩選所得cdna片段和ap - 2 cdna全長及ap - 2 cdna的分段形式用gst基因融合系統進行表達，並用ap - 2的單克隆抗體檢測了gst - ap - 2融合蛋白表達的情況。 -
Bevacizumab, a monoclonal antibody against vascular endothelial growth factor, has shown promising preclinical and clinical activity against metastatic colorectal cancer, particularly in combination with chemotherapy
背景：一種新型的抗血管內皮單克隆抗體貝伐單抗在基礎以及臨床研究中均對轉移性結腸癌表現出良好的治療效果，尤其在與其他化療藥物結合時效果更佳。 -
2. monoclonal antibody gl biochem provides the following hybridoma development program
吉爾生化從2005年2月起開始提供抗體制備服務。 -
It adopts the au - labeled monoclonal antibody technology. it has high sensitivity, excel distinctiveness, accurate result and is easy to operate. it widely applies to clinical diagnosis in hospital, self - diagnosis in home, general survey of birth planning and so on
它運用單克隆抗體金標技術研製而成，直接檢測尿液，不需晨尿，具有靈敏度高特異性強操作方便結果清晰等諸多優點，廣泛適用於醫院臨床診斷計劃生育普查及家庭自診等。 -
After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。 -
By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles
對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。 -
In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company
實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 （ 1 : 1 ）混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑（ upofectalnineplusreageni ）和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 （ do一7 ）蛋白（即用型） ;鼠單克隆抗體p21waf ， （即用型） ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰（濃縮型） ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。 -
The neurites were also identified by immunochemical staining using monoclonal antibody to neurofilament
利用免疫化學染色，這些生長的神經軸突可被對抗神經細絲的單株抗體所染色。 -
The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus
結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中， meq蛋白僅局限於細胞核內，而且隨著感染后（ pi ）時間的增加，具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。 -
Methods according to theory of specific binding of antigen and antibody, at first the anti - a monoclonal antibody ( ma ) and anti - bma were labeled with the fluorescent, then fluorescent - labeled antibodies ( fla ) were bound with corresponding biological material ( such as bloodstain ) in the optimum condition, finally the abo blood type of bloodstain was determined under microscope fluorescent
方法根據抗原抗體特異性結合的原理，首先對抗a 、抗b單克隆抗體進行熒光標記，然後使熒光標記抗體與相應抗原（血痕）在最佳條件下結合，最後熒光顯微鏡鏡檢，判定血痕的血型。
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