mpem 中文意思是什麼
mpem
解釋
姆佩姆河-
Objectives : to express mpem protein in e. coli
目的：用大腸桿菌表達mpem蛋白。 -
The fusion protein had expected primary structure. conclusion : pgex - 4t / mpem expression plasmid was constructed. gst / mpem fusion protein was successfully expressed
結論：構建了原核融合表達質粒pgex - 4t mpem ；成功在大腸桿菌中表達gst mpem融合蛋白。 -
Then it was subjected to hydroxylamine cleavage to verify the predicted primary structure. result : gst / mpem fusion protein was expressed successfully in e. coli. we obtained certain amount of gst / mpem protein by means of affinity chromatography
結果：在大腸桿菌中誘導表達出gst mpem融合蛋白，經過親和層析獲得一定量的gst mpem融合蛋白，它們具有預期正確的一級結構。 -
Methods : the mouse pem gene ( mpem ) cdna coding sequence was cloned into prokaryotic gst fusion protein expression plasmid pgex - 4t - 3. the recombinant plasmid was transformed to e. coli bl21 and the gst / mpem fusion protein was induced to express with iptg. the fusion protein was purified by affinity chromatography
方法： pcr擴增小鼠pemcdna編碼序列，將它克隆到含有gst的原核表達質粒載體pgex - 4t - 3上，轉化大腸桿菌bl21 ，誘導表達gst mpem融合蛋白，通過親和層析，獲得初步純化的產物，以羥胺切割驗證其一級結構。
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