ms-pcr 中文意思是什麼
ms-pcr
解釋
突變分離pcr-
Pcr method was used to identify candidate ms 188. sequence analysis indicated that a point mutation occurred in the second exon of the atmyb103 gene in male sterile mutant with caa ( gln ) replaced by a stop codon taa
利用pcr的方法從突變體中擴增atmyb103基因並進行序列分析,結果表明突變體中atmyb103基因第二個外顯子上發生了點突變,由原來編碼谷氨酰胺的caa密碼子突變為終止密碼子taa 。 -
The room for the following equipments need the air - conditioning : uv - spectrophotometer, fluorescence spectrometer, gc, gc - ms, aas, afs, icp, icp - ms, hplc, hplc - ms, ion chromatography, atomic fluores - cence spectrometer, pcr amplification and balance
12紫外/可見分光光度儀、熒光分光光度,氣相色譜,氣相色譜質譜、原子吸收,原子熒光儀、等離子發射光譜、等離子質譜、高效液相、液相色譜質譜、離子色譜、熒光光度、 pcr核酸擴增儀以及天平室安裝空調。 -
2. construction of chimeric mtb8. 4 / hil - 12 eukaryotic expression plasmid ( 1 ) construction of pci - neo - mtb8. 4 - linker ( pml ) and pci - neo - ms - linker ( pmsl ) mtb8. 4 - linker and ms - linker gene ( without stop codon ) were pcr amplified by using two oligonucleotides designed to generate nhe i and mlu i restriction sites at the 5 " and 3 " ends of the amplified fragments, respectively
3 .重組質粒在真核細胞中的表達: pm 、 pms 、 pmi和pmsl重組質粒用lipofectaminatmzo0o脂質體轉染試劑轉染cos一7細胞,進行瞬時表達, 48小時后,用rl 』 - pcr檢測目的基因在mrna水平的表達;用westemblotting檢測hil一12在蛋白質水平的表達。 -
Study of gc - subtype genotyping by multiplexed ms - pcr and application
細胞脹亡及其研究進展 -
Sequences flanking tn5 in nm21 was cloned by anchored pcr. a 3073bp fragment which contains three putative open reading frames ( orf ) and shows strong homology with the magnetospirillum magnetotacticum ms - 1 was obtained, the tn5 inserted in the orf1
用錨定pcr法從nm21中克隆出tn5側翼序列,得到一個大小為3073bp的dna片段,此片段由3個orf組成,與m . magnetotacticumms - 1的對應片段具有89的同源性。 -
Ms result was consistwt to the result of rt - pcr detection
程和瓊脂糖凝膠電泳所需要的大量時間。
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