mtt 中文意思是什麼
mtt
解釋
甲基噻唑基四唑-
Mtt value, morphological characteristics under light and electron microscopy, dna gel electrophresis results, and hoechst33342 dying results show that inosine can protect nscs against apoptosis induced by hioi significantly in a dose dependent manner
2的直接保護作用，通過mtt值的測定，光鏡及電鏡的形態觀察， hoechst33342染色后核形的觀察及dna凝膠電泳結果，發現肌昔可對hzoz誘導的c門 -
Use trypan blue exclusion method and mtt colorimetric method to detect u 2 - os cell growth inhibition caused by treatment of bfgf. 2
採用臺盼藍排除檢測法和mth比色法檢測bfgf對u2 os細胞生長的抑制情況。 -
Five analogues and five segments were designed and synthesized by using solid phase synthesis method according to separated papaver somniferum pollen tridecapeptide with antitumor activities as leading peptide. their primary secondary structures in solution were determined by cd spectra and their inhibitive activities to human liver and mammary gland cancer cells were assayed by mtt method. the relationship of structure - activity was studied and discussed
罌粟花粉十三肽對人肝癌和人乳腺癌腫瘤細胞具有明顯的抑制作用，以其為先導化合物，設計併合成了5個類似物和5個片段，結合cd譜測定的二級結構及它們對人肝癌和人乳腺癌腫瘤細胞的抑制作用測定結果，研究並討論了該肽結構與抗腫瘤活性的關系 -
Mdr1 express product p - glycoprotein was detected by immunocytochemical method and flowcytometry. the cytotoxicity and multidrug resistance reversion effect of tea polyphenol was examined by mtt assay in mcf - 7 and mcf - 7 adr carcinoma cell lines, and compared with pgp inhibitor quinidine. the pgp expression of mcf - 7 adr was strongly positive, the positive rate was 15 % ; the pgp expression of mcf - 7 was negative, the positive rate was 1. 8 %. ic50 of tea polyphenol to mcf - 7 and mcf - 7 adr is 115. 2g ml and 207. 6g ml respectively. ic50 of quinidine to mcf - 7 and mcf - 7 adr is 129. 8mol l 42. 1g ml and 94. 1mol l 30. 5g ml respectively. tea polyphenol and quinidine changed little toxicity of adriamycin to mcf - 7, but tea polyphenol and quinidine improved the sensitivity of mcf - 7adr to adriamycin significantly. immunocytochemistry and flow cytometry can detect p - glycoprotein expression level qualitatively and quantitatively. tea polyphenol is not only an anti - tumor agent, but also a multidrug resistant modulator similar as quinidine. tea polyphenol is advantageous for its little toxicity in tumor treatment
用免疫組化法和流式細胞儀對腫瘤細胞系mcf - 7和mcf - 7 adr的p -糖蛋白表達水平進行定性定量研究。用噻唑藍比色法mtt研究茶多酚的細胞毒性及其對耐藥性的逆轉作用，並與pgp抑制劑奎尼定進行了比較。免疫組化法檢測p -糖蛋白表達水平， mcf - 7 adr呈強陽性，而mcf - 7呈陰性流式細胞儀定量檢測結果mcf - 7 adr細胞系細胞陽性率為15 % ， mcf - 7細胞系細胞陽性率為1 . 8 % 。 -
To investigate the mechanism of trefoil factor 3 on the gastric intestine epithelial restitution, the recombinant human trefoil factor 3 was added to human colonic tumor cell and the proliferation effect was examined by mtt assay. the recombinant protein didn t promote the proliferation of the hct cells at low density of 0. 010. 05 g l and only has weakly proliferation effect at density of 0. 10. 2 g l. 1 g l of the recombinant protein could significantly promote the cell migration of hct cells when added to the monolayers cells
將重組人三葉因子3 trefoil factor 3 ， tff3作用於人結腸腫瘤細胞，研究重組蛋白對細胞增殖的影響，結果發現該蛋白在較低的濃度1050 mg l下對細胞的增殖基本沒有影響，在100200 mg l濃度下該蛋白對細胞僅有微弱的刺激作用，提高濃度對細胞增殖作用沒有改變。同時研究了tff3對損傷的單層結腸腫瘤細胞遷移的影響，發現tff3對細胞有明顯的促進遷移作用。 -
To study the suitable method for cattle oviduct simple epithelium cells culture, the epithelium cells were isolated by cutting and 0. 25 % trypsinization, the exponential phase of growth cells vigor and growth velocity was determined by mtt method, the viable count was detected by the rejection experiment of trypanblau
摘要為探討適用於黃牛輸卵管單層上皮細胞的培養方法，採用機械剪取及0 . 25 %胰酶消化的方法分離獲得上皮細胞，取對數生長期細胞進行mtt比色實驗檢測細胞活力和生長速度；臺盼藍排斥試驗檢測活細胞數。 -
In the algorithm, the theory of multi - targets tracking ( mtt ) was induced to recognize and tracking obstacles
在該方法中，作者針對alv障礙檢測的實際要求，將多目標跟蹤的理論和思路引入到演算法裏面來。 -
The cell proliferation was observed with flow cytometer and mtt colorimetric test
Atra明顯拮抗了雌激素對細胞增殖的正性調節作用。 -
Surface markers on dcs were then analyzed by flow cytometry and the proliferation of t cells was detected by mtt colorimetry. resoults : peripheral blood monocytes from patients of carcinoma treated with rhgm - csf of 1000 u / ml plus il - 4 of 500 u / ml for 7 days could observe dcs with typical morphology. simultaneously there was a decrease in cd 14 expression and increase in hla - dr, cd40, cd83 and cd86 on dcs
結果，癌癥患者外周血中的單核細胞在rhgm - csf1000u ml + il - 4500u ml的條件下培養一周，就可看到典型的樹突狀細胞形態，其表面cd14分子表達減少， hla - dr 、 cd54 、 cd40 、 cd83及cd86分子的表達明顯增高，且具有明顯刺激t細胞增殖的能力，成功地完成了外周血單核細胞來源的dc的培養。 -
The survival - rate of the recovered cells was tested by mtt and fda - pi. therefore, we got the preferable condition of vitrification, i. e., the concentration of cryoprotectant is 60 % and the time of disposal is 15min. under this condition, the value of fda / pi is 46. 43
凍融后的細胞經過mtt檢測和fda - pi雙熒光染色法檢測，獲得預處理過渡的優化條件，即60濃度的保護劑，預處理15分鐘，此時的fda pi值為46 . 43 。 -
Cell compatibility of films is researched firstly, which will make a significant contribution to the using fha films in practice from development. cell cycle, measured by flow cytometer and mtt method, and cell growth curve are used to analyze the impact of material and the immersed medium to the multiplication of osteoblast - cell
通過mtt法，流式細胞儀測定細胞周期，以及細胞生長曲線的測定，分析研究了fha薄膜材料對成骨細胞增殖生長的影響以及材料的浸提液對細胞增殖的影響，通過細胞相對增殖活性的測定對fha薄膜進行毒性評級。 -
The cytotoxicities of rta and the fusion toxin rta - yqrl were measured by the mtt assay in hela, skov - 3, and wish cells following fluid - phase endocytosis. [ methods ] 1
因為rta可與f3ga發生特異性結合，我們用bule一sepharose一6b柱對rta和rta一yqrl蛋白分別進行親和層析純化。 -
Fiorina holds a bachelor ' s degree in medieval history and philosophy from stanford university ; a master ' s degree in business administration from the university of maryland at college park, md. ; and a master of science degree from mtt ' s sloan school
菲奧里納曾獲斯坦福大學的中世紀歷史和哲學學士學位，馬里蘭大學的商業管理碩士學位，以及麻省理工大學斯龍學院的理科碩士學位。 -
Part i effects of gnt - v on cell proliferation, cell sensitivity to egf and the egf receptor of h7721 cell line using mtt method, it was found that the proliferation of cells transfected with sense gnt - v cdna was facilitated, and both of the total 3h - tdr incorporation and the specific incorporation per cell were also increased. oppositely, these parameters were reduced in cells transfected with antisense cdna of gnt - v. these results suggested that cell proliferation and dna synthesis were modulated by gnt - v
第一部分gnt - v對h7721細胞生長、 egf敏感性和egf受體的影響用mtt方法發現轉染正義gnt - vcdna的h7721細胞增殖速度加快，而且無論是~ 3h - tdr的總參入量或每個細胞的參入量均見增加，說明dna合成增強，而轉染反義gnt - vcdna的h7721細胞則完全相反， dna合成和細胞增殖速度均見降低，這提示gnt - v可調節細胞的生長。 -
Biological activity was determined by egf dependent balb / c 3t3 cell line and with mtt colorimetric assay. extracts of the recombinant virus - infected and mock - infected cells, haemolymph of the recombinant virus infected and mock - infected silkworm larvae could all support the proliferation of balb / c 3t3 cell. this phenomena implied that there were some egf - like growth factors in the haemolymph of normal silkworm larvae, which could enhance the proliferation of the cell line
用小鼠balb c3t3成纖維細胞和mtt法測定表達產物的促細胞增殖作用，發現重組病毒感染家蠶細胞72小時的胞內樣品與正常家蠶細胞裂解物，以及重組病毒感染4天的蠶血淋巴與正常蠶血淋巴均具有相似的促細胞增殖作用，甚至野生型病毒感染的細胞裂解物和蠶血淋巴也有一定的細胞促生長作用，提示家蠶系統本身可能含有能促進細胞生長、類似於egf的細胞因子。 -
In vitro endothelial proliferation inhibiting activity of recombinant angiostatin was examined with mtt method by using human umbilical vein endothelial cells ( huvec ). in this test we could draw the inhibition curve and calculated the ic50 to confirm its bio - activity further we do cam vascular inhibition test in vivo
4驗證重組蛋白angiostatin的生物活性：採用mm法測定重組蛋白對原代培養的人臍靜脈內皮細胞（ huvec ）的抑制作用，繪出抑制曲線，並計算出ic 。 -
Methods for qualitative and quantitative characterization of primmorph culture were developed, which included a modified mtt assay for measurement of cell viability and cell growth, a 5 - brdu - incorporation assay for detection of cell proliferation, propagation and metabolism of in vitro primmorph
系統考察並優化了mtt法應用於海綿細胞培養過程細胞活性和細胞生物量定量的條件，結合5 - brdu摻入法建立了細胞團培養過程中細胞增殖能力和細胞活性的評價方法。 -
In this study, we use immunohistochemistry, electron microscope, cell culture, image analysis, mtt method and radioimmunoassay to study the localization, varies of quantitation, and possible frictions of 5 - ht and subtype of 5 - htr in human placenta. the results were as follows : 1
本研究用免疫組織化學、免疫電鏡、細胞培養、圖象分析、 mtt法和放射免疫等技術對5 -羥色胺及其受體亞型在胎盤絨毛中的細胞定位及對滋養層細胞增殖和激素分泌的影響進行了初步的探討，其結果如下： 1 -
Mtt colorimetric assay of corneal epithelial cell activity
比色法檢測兔角膜上皮細胞活性 -
In cultured neonatal rat cardiac myocytes, mtt methods, total protein measurement and 3h - leucine incorporation were used to evaluate the cell number and protein synthesis of cardiac myocytes
脯氨酸摻入實驗測定膠原合成速率，放免法測定細胞內cgmp和。 amp ，定量pcr技術研究vnp對cfs的npr c的調控。
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