mutant allele 中文意思是什麼
mutant allele
解釋
突變等位基因：任何和野生型基因不同的基因。-
Of the 21 patients conservatively treated between 1975 and 1997, 12 had diffuse disease ( homozygous mutant alleles ) and 9 had presumed focal disease ( paternal mutant allele only )
1975年至1997年期間接受保守治療的21例患者中， 12例為彌漫性病變（等位基因突變純合子） ， 9例為局灶性病變（僅父系等位基因突變） 。 -
There are no obvious difference for the frequencies and genotypes distribution for the mbl gene codon 54 mutant allele between hans and uigurs
10 ，兩民族ggc54gac基因型分佈和突變頻率無顯著差異。 -
By using single strand conformation polymorphism analysis of polymerase chain reaction ( pcr - sscp ), the frequency of codon 54 mutant allele of mbl structural gene in the population of hans was investigated. 3. the molecular beacon ( mb ) hybridization technique with visual monitoring was established
建立聚合酶鏈反應單鏈構象多態性分析inglestrandconforma tlonpolymorphlsmanalysisofpol仰erasechainreactionproduction ， pcr sscp ）方法，初步調查漢族人群mbl基因ggc54gac突變情況。 -
Genome dna of all blood samples were extracted by using erythrocyte lysis solution and pcr buffer / nonionic detergent / proteinase k. a 109bp fragment was amplified from the human genome dna. 2. in 166 han samples, 19 ( 11. 45 % ) heterozygous genotypes of codon 54 mutant allele were found by pcr - sscp
用紅細胞裂解液和pcr緩沖液非離于去污劑蛋白酶k兩種自配試劑成功地提取了所收標本的人白細胞基因組dna ，並以此為模板成功擴增出預期的109hpmbl目的基因片段。 -
The frequency of codon 54 mutant allele in exon 1 of mbl structural gene is 0. 13 in the population of hans, while that is 0. 10 in uigurs
漢族人群mbl結構基因第一外顯子ggc54gac突變頻率為0月3 ，維吾爾族的突變頻率為0 -
Whereas their parent mutant axr1 - 3 was male fertile, showed moderate insensitivity to ja - inhibitory root growth and mild reduction of ja - inducible gene expression ; parent mutant coi1 - 2 - was a leaky mutant allele of coi !, it exhibited reduced ja insensitivity and partial fertility. - taken together, all these results suggested that scfcoil complex was required for the ja response and that the axr1 - dependent rubl modification of atcull subunit of scfcol " was important for ja signaling in arabidopsis
試驗結果發現， coil 2 axrl 3雙突變體在根的生長、茉莉素誘導基因的表達和雄性育性方面對茉莉素都表現為完全抗性；而親本coil 2和axrl三突變體對茉莉素均表現部分不敏感性，表明axr基因和coil基因的同時突變協同增強擬南芥對萊莉素的不敏感性， axr基因與coi基因相互作用調控茉莉素的信號傳導途徑。 -
2. to establish the frequency of codon 54 mutant allele and compare the difference of genotypes of mbl structural gene in the population of hans and uigurs
利用該技術對漢族、維吾爾族人群mbl結構基因第一外顯子ggc54gac突變進行檢測，並估算其基因頻率，比較漢族、維吾爾族該位點突變的差異。 -
To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。
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