mutant gene 中文意思是什麼
mutant gene
解釋
變異基因-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。 -
Because the phenotype of osdd2 mutant was segregated with the basta resistance, it suggested that the mutation was arose by disruption of the wrky gene
根據已有的共分離數據和初步分析結果表明osdd2突變體的發育遲緩性狀是有外源基因插入引起。 -
Expression and cloning of human glucagon - like peptide - 1 mutant gene
7基因的克隆及序列分析 -
Furthermore, the wing imaginal disc clonal analysis showed that in the homozygous sll mutant clones, the extracellular wg was dramatically reduced. the reduction of extracellular wg indicated that the wg signaling in the wing imaginal disc was disrupted. the reason leaded to this phenomenon is that sll encodes a paps transporter, so the disruption of the sll gene would generate the unsulfated heparan sulfate proteoglycan ( hspg ), which most likely would lose normal function
進一步結合運用flp - frt系統和minute ~ - ，用缺損myc蛋白的表達來標記純合的sll突變基因克隆，用minute ~ -來相對的擴大含sll突變基因的克隆，對翅成蟲盤所作的克隆分析表明，在含sll突變基因的克隆中，細胞外的wg蛋白分佈明顯減少，這種細胞外的wg蛋白減少表明在翅成蟲盤中wg信號傳導受到了明顯抑制。 -
This group of mutant alleles turned out to be a gene encoding a golgi adenosine 3 ' - phosphate 5 ' - phosphsulfate transporter ( adenosine paps transporter ). kyte - doolittle hydrophilicity plots showed that sll is a very hydrophobic protein, especially in c terminus
Kyte - doolittle疏水性分析結果表明，與其它轉運子相似， sll蛋白是一個疏水性很強的蛋白（尤其在它的c端） 。 -
By using the complementation test an unknown mutant can be rapidly assigned to a particular gene.
採用互補測驗可迅速地將某一未知突變型歸屬于某一特定基因。 -
Someone tried this way but the yield is low. francis expressed truncated mutant pokeweed antiviral protein gene in pichia pastoris
目前已經有人在這方面做出了嘗試，但蛋白表達產量還不高。 -
Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。 -
4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。 -
Ddrt - pcr method was exploited to study the differential gene expression between immature siliques of arabidopsis wide - type and ast mutant
採用ddrt - pcr的策略，分析野生型與突變型植株未成熟角果中基因表達的差異。 -
In other words, depending on where in the brain the mutant gene is expressed, it can result in different types of the condition ? “ higher ” synesthesia, driven by numerical concept, or “ lower ” synesthesia, produced by visual appearance alone
換句話說，隨著變異基因在腦中表現位置不同，就可造成不同種類的情況，包括由數字概念所引發的較高等聯覺，或就只是由視覺外形造成的較低等聯覺。 -
Mutant gene affected m - centrin propertities. from uv different spectrum, we know that there are fine absorption of tyr and trp apart from those of phe
實驗表明：從紫外吸收圖譜看，突變之後的中心蛋白中除了苯丙氨酸的精細結構外還有酪氨酸和色氨酸的吸收峰。 -
Preparation and identification of recombinant adenoviral vectors containing human wild type spk and its mutant gene
攜帶人鞘氨醇激酶及突變體基因重組腺病毒載體的制備及表達 -
Butterfly bettas have the mutant gene that causes variegated fins with a very specific fin pattern
蝴蝶斗魚因基因突變擁有色彩斑駁的鰭及特殊的花色。 -
After its sequence was determined, the mutant gene was cloned into a shuttle vector pnov - r and transformed into br - deficient halobacteria ( l33 )
突變后的蛋白經生物信息學軟體分析了和野生型br的不同。 tyr79 argbr突變體的光電特性還要進一步分析和測試。 -
The fragment containing mutant gene was subcloned into suicide vector pkng101, named pkh6, pkh 7 and pkh8 respectively. moreover, eaggec 17 - 2 was used to construct gene mutants by homologous recombination
利用自殺質粒pkng101作為載體，構建了ip氏ip7和if 』 ps基因重組自殺質粒pkll6 、 pkh和嚇。 -
Actually, it ' s males that carry the mutant gene and pass it on, so it ' s his fault
實際上，是雄性遺傳攜帶突變基因，所以都是他的錯 -
It may arise naturally due to mutation in a cell of a developing embryo, producing a line of cells with the mutant gene, and hence different characteristics compared to surrounding cells
嵌合體可以由發育著的胚胎細胞變異而自然形成，這樣就產生了具有不同性狀的變異基因的細胞系。 -
Scientists such as ourselves are trying to offer hope to families with huntington ' s by working to understand more fully how the mutant gene causes the disease and how it might be circumvented to provide a treatment
至於我們這些學者，則致力於深入了解該突變基因究竟如何引起這種疾病，以及有什麼法子可以戰勝它、提供治療之道。 -
If that is shown to be true for humans as well as mice, it could pose a problem for the mutant - gene theory of cancer
如果人類也和小鼠一樣具有這個現象的話，癌癥起於突變基因的理論將出現問題。
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