mutant strain 中文意思是什麼
mutant strain
解釋
突變菌株-
Through a series of mutagen tests, a mutant strain of monascus spp ( m 12 - 69 ) from ml2 was acquired, which can produce much higher monacolin k than m12, and almost same citrinin level as m12
以之為出發菌株，通過誘變篩選，獲得一株monacolink產率有明顯提高而桔黴素產率僅為0 . 13ng g （ elisa ）的突變株，編號為m12 - 69 。 -
Breeding of highly productive - linolenic acid mutant strain of mortierella ramanniance
亞麻酸高產突變株的選育 -
Breeding of alkaline lipase overproducing strain by screening resistant mutant
抗性突變株篩選法選育堿性脂肪酶高產菌 -
4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物奎尼酸合成方向，利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。 -
The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku
將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。 -
A strain of the mutant mgxcr1 with a much lower level of cu resistance was selected by ultraviolet mutagenesis from the wild type strain gxcr1
用紫外誘變法獲得了gxcr1的銅抗性水平明顯降低的一株突變體mgxcr1 。 -
In this study, lexa mutant of deinococcus radiodurans xe has been successfully constructed. the survival curve of this strain was measured using colony formation assay after treatment with different doses of radiation and different times of mmc
本研究利用已構建的抗輻射菌lexa基因突變體菌株xe ，經受不同劑量射線照射和不同時間的mmc作用，平板菌落計數，繪制存活曲線。 -
The page revealed the culture supernatant of the initial strain and the mutant contained different protein bands, which exactly demonstrated at protein level that a. niger j 506 was surely a mutant of a. niger m1. zymogram stained with xylan - remazol brilliant blue for detecting xylanase showed there are three different xylanases in the mutant culture, while two xylanases in initial strain. what is important, the third xylanase in a. niger j 506 have higher activity and more production levels from page and zymogram of xylanase
尤其是在木聚糖酶譜帶檢測中發現，突變株發酵液中有三種類型的木聚糖酶，而出發菌株中只有兩種類型的木聚糖酶，並且通過考馬斯亮藍g250染色和瓊脂糖板上的透明圈發現，突變株中第三種類型的木聚糖酶不僅表達量很大，活力也很高。 -
Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b, in which the yields of avermectins b in 82 isolates were only about 0. 1 ~ 2 % of those produced by the parental strain olm73 - 12. 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis. the mutant only producing avermectins bl was not detected
搖瓶發酵和hplc分析結果表明，有99個突變株仍然產阿維菌素b的4個組分，其中82株的發酵單位很低，僅為出發菌株olm73 - 12的0 . 1 2 ，從中挑取2株經pcr擴增和測序驗證，均發生了正確的基因取代；沒有檢測到僅產b1的突變株，這表明阿維菌素b2組分的產生並不是因為阿維菌素pks上dh2的部分活性所造成。 -
The morphological, physiological, biochemical and genetic diversities between bacillus thuringiensis wild - type strain ybt - 1463 and its plasmid - free mutant bmb171 was comparatively studied. it showed that the plasmid - free mutant strain bmb171 lost the ability to form the parasporal crystal, but there was on obvious diversities were observed on the sensitivity to 10 antibiotics, the utilization of 19 carbon sources and 12 nitrogen sources, as well as the growth properties between ybt - 1463 and bmb171, whereas the electro - transformation frequencies of bmb171 were much higher than those of ybt - 1463, respectively with 5 exogenous plasmids as the donor dnas
對出發菌株ybt - 1463和其無質粒突變株bmb171的部分形態、生理生化和遺傳學特性進行的比較研究的結果表明，突變株bmb171不形成伴胞晶體，但在個體形態與菌落特徵、對紅黴素等10種抗生素的敏感性、對葡萄糖等19種碳源和谷氨酸等12種氮源的利用能力及生長性能與出發菌株ybt - 1463無明顯差異。 -
Studies on morphological observation and categorization showed that there were several differences between the strain m12 and the mutant strain m12 - 69, but they both belong to monascus pilosus sato, according to hawksworth and pitt
第四，根據hawkswo曲和pitt關于紅麴黴的分類方法，對m12及m12一69的培養物進行了形態觀察及分類鑒定。 -
By treatment with both ultraviolet rays and licl, a mutant strain b. s. lx - 19 with high yield of heparinase and stability was obtained, whose enzyme activity increased by 40 % over strain lx - 10
Lx ? 19 ，其肝素酶產量較出發菌株提高了40 。通過單因素條件研究，發現n源和c源對酶產量影響較大，接種量以10 ，初始ph6 . 5 ，種齡12h為宜。 -
Breeding of mutant strain for glulamate prodaction
谷氨酸生產菌的選育 -
Plant assay was performed with the wild - type strain gx201, the mutant strain gx217 and the function recovered strain rgx217. the results indicate that the mutant strain gx217 showed a day delay in nodulation time and its abilities of nodule formation efficiency and competitive nodulation were apparently decreased compared with the parental strain gx201 respectively
通過對野生型菌株gx201 ，突變體菌株gx217及其功能互補菌株rgx217進行植株試驗，發現突變體菌株gx217在結瘤時間方面較野生型菌株gx201慢一天，其結瘤效率及競爭結瘤能力均比gx201明顯降低。 -
A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally
進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。 -
The recombinant plasmids pbmb121l, pbmb9821l and pbmb986l were constructed after transferring bacillus thuringiensis icps gene crylaal, crylaclo and crylca from their original plasmid vectors to different plasmid vector. the relevant recombinant strains were obtained after introducing the 3 recombinant plasmids into bacillus thuringiensis plasmid - free mutant strain 8mb 171 by electroporation. the transformation and expression properties of 8mb 171 were studied
通過將蘇雲金芽胞桿菌殺蟲晶體蛋白基因cry1aal 、 cry1ac10和cry1ca轉移至不同質粒載體上，分別構建了重組質粒pbmb121l 、 pbmb9821l和pbmb986l ，並分別導入無質粒突變株bmb171 ，篩選得到攜帶相應icps基因的重組菌株。 -
Nutrient value of protein from spores of agaricus blazei mutant strain j
3孢子中蛋白質的營養價值評價 -
( 4 ) expressional plasmids containing the cloned pha synthase genes were constructed and transformed into wild type e. coli strains and e. coli fadb deleted mutant strain km32b
（ 4 ）成功構建了含有上述型pha合酶基因的表達載體，並將它們分別轉入野生型大腸桿菌及脂肪酸降解fqdb缺夫突變株km32b中進行表達。 -
Breeding of mutant strain for glulamate production by changing its metabolic passway
通過改變代謝途徑選育谷氨酸生產菌株 -
Between 918 and 1180 aa, 22 - 23 amino acids of crylaal4 were different from 11 of all 13 crylaa protein except cry1aa9 and cry1aa13, and especially four amino acids were additional, but they could be found in crylab protein. recombinant plasmid, pbybl, with full length cry1aa14 gene was constructed and transformed into a bt acrystalliferous mutant strain hd73 cry
在氨基酸序列918 1180間，和以知的13種cry1aa的11種（不包括cry1aa9和cry1aa13 ）存在22或23個氨基酸的差異（其中1094 1097的4個氨基酸無對應序列） ，而這段區域和cry1ab氨基酸序列的對應區域無差異。
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