ndv-f 中文意思是什麼

ndv-f 解釋
毒株
  • f : 1 【化學】 fluorine 2 【生物學】 （generation of） filial offspring; 【植物；植物學】子代 〈F1 雜...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Construction of recombinant fowlpox virus coexpressing aiv ha and ndv f. for the construction of transfer vector pfgs11haf, aiv ha gene of f strain in puc18ha and ndv f gene of f48e8 strain in puc19f were removed and inserted into pfgs11. recombinant fowlpox viruses ( rfpv ) coexpressing aiv ha gene and ndv f gene were constructed by using different promoters of ps and pe / l. recombinant rfpvs were derived by dosper liposome - mediated transfection with the two transfer vectors on chicken embryo fibroblast ( cef ) monolayer cultures which were infected by wild type fpv chinese vaccine strain 282e4 3 - 4 hours earlier

    Puc18ha和sk質粒同時經hind 、 kpn酶切后連接得中間質粒skha ；將質粒skha用bamhi酶切回收ha基因插入到插入載體pfgs11中的bamhi位點，通過酶切鑒定獲得了pfgs11ha ；將含ndvf基因的質粒puc19f用hind 、 sal酶切經klenow酶補平插入到經sma酶切后的skifn中pe / l啟動子下獲得中間質粒skf ，再將質粒skf和puc18質粒先分別用ecor 、 xho酶切klenow補平，后再共同用sac酶切連接得puc18pelf ， sal酶切回收pe / l - f基因盒插入到pfgs11ha的sal位點，通過酶切鑒定獲得了pe / l - f與ps - ha同向的表達載體pfgs11haf 。
  3. Newcastle disease virus ( ndv ) is grouped under the family of paramyxoviridae, it causes serious infectious disease and incurred great losses to poultry industry. f and hn protein were main glycoproteins, which affect the virulence of the virus

    新城疫病毒（ newcastlediseasevirus ， ndv ）屬于副粘病毒科（ paramyxoviridae ）副粘病毒亞科（ paramyxovirinae ）腮腺炎病毒屬（ rubulavirus ）的成員，其主要結構蛋白融合蛋白（ f ）和血凝素-神經氨酸酶（ hn ）與其致病性密切相關。
  4. 374 - nt sequence analysis between nt 47 - 420 and restriction enzyme ( re ) clevage site mapping of f gene between nt 34 - 1682 were used to compare the 18 isolates for genetic analysis. a phylogenic tree was constructed based on the 374 - nt - sequence data of eighteen isolates in the study and 37 ndv reference strains from genbank and published resources

    通過dnastar軟體對f基因47 470nt間片段進行同源性分析比較並繪制了遺傳進化樹枝狀結構發生圖，結合334 1672nt間三種限制性內切酶（ re ： hinf ， bsto及rsa ）位點的分佈情況，確定了這些分離株的基因分類地位。
  5. Phylogenic tree from 29 strains were draw based on the ndv hn gene ( part segment ), and the result is similar to that of f gene

    根據29株ndvhn基因部分編碼序列繪制ndv系統發育進化樹，發現f及hn基因的分型結果大致相同。
  6. The fusion ( f ) protein and hemagglutinin - neuraminidase ( hn ) are the major virulence factors of the virus to which the host responds well with protective antibody production. recombinant plasmids containing the f or hn gene can induce specific immune responses against ndv. however, dna vaccination by injection or gene gun routes is rather inconvenient and costly in field applications in scaled animal farms

    融合蛋白（ f ）和血凝素-神經氨酸酶蛋白（ hn ）是ndv的主要宿主保護性抗原，以f基因和hn基因為免疫原研製的dna疫苗能誘導一定程度的免疫應答，但注射免疫或基因槍免疫途徑因實際操作和成本等問題難以推廣應用，而直介面服重組質粒dna免疫效果不理想。
  7. A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses

    依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。
  8. The result further proposed that different genotypes of ndv had not directed the antigenic changes of f protein, the most important protein of ndv in the protective immune response of the fowls. protective efficacy difference among various genotype ndv vaccines of different origin was studied. except the vaccine strain lasota ( genotype ii ), the challenge strain f48e8 in china, the ndv strains tested were isolated from nd outbreaks in chickens, gooses, and pigeons

    通過不同來源不同基因型（亞型） ndv滅活苗與常規疫苗（包括lasota弱毒活疫苗和滅活疫苗）的免疫保護效力的比較，證實了所有疫苗均能激發機體產生抗不同基因型ndv的抵抗力， hl抗體變化都具有一個抗體滴度迅速上升的過程，動態特徵除lasota弱毒活疫苗組有些不同外相當一致。
  9. 22 strains of ndv of four genotypes ( ii, vi, vii, and viii ) of chicken, goose, and pigeon origin, were studied by the means of seven molecular antibodies prepared against the ndv f protein. weak antigenic heterogeneity was found among these strains and the difference beared no relation to different genotypes

    根據針對ndv融合蛋白（ f ）的7株單克隆抗體與22個不同基因型不同來源ndv毒株的反應情況，初步證實這些ndv毒株f蛋白存在抗原表位的細微差異，但這種差異性與不同基因型間無必然的相關性。
  10. In order to study immunization of genetic vaccine by inoculation, two kinds of dna vaccine of ndv are constructed and analysed their sequense, which will express f protein in eukaryotic cell ( hela cell ) in vitro with the method of transfection by cationic liposome the results testify that two sorts of plasmids dna successfully expressed the f protein of ndv in hela cell, but the expressed content of pc4. 0f is higher than ones of pc3. 1f

    將構建的基因疫苗pc3 . 1f大量提取和純化，採取肌肉注射和腹腔注射兩種途徑、不同劑量免疫小鼠，用elisa方法檢測小鼠血清中抗f基因表達產物的抗體水平。研究結果表明，基因疫苗pc4 . 0f和pc3 . 1f均在hela細胞中成功表達，其中pc4 . 0f的表達量比pc3 . 1f高。
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