nicotiana 中文意思是什麼

nicotiana 解釋
花煙草
  1. The true alkaloid nicotine in nicotiana functions as an allomone against many insect species.

    煙草屬中的真生物堿,煙堿作為利已激素,能防治許多昆蟲。
  2. Toxicity was reduced when nicotiana trichome exudates were removed by various washing methods.

    當通過不同洗滌方法除去煙草屬毛狀體的分泌物時,毒力降低。
  3. This paper describes a strategy that has developed to transfer the cdna of tobacco mnsod gene into the commercially important breeding line - baoding alfalfa via agrobacterium infection. transgenic alfalfa plants have been generated that overproduce a nicotiana plumbaginifolia l. manganese superoxide dismutase ( mnsod ). the results domenstrated that baoding alfalfa is an important breeding line which easily amenable to genetic transformation

    本研究採用我國農藝性狀優良的豐產苜蓿品種保定苜蓿,通過農桿菌介導的轉基因方法,使用特定的質粒載體pchlsod將煙草mnsod基因的cdna序列導入保定苜蓿中,說明保定苜蓿是一種易於遺傳轉化的優良苜蓿育種品系。
  4. Leaves of tobacco ( nicotiana tabacum ) were wounded, infected by agrobacterium tumefaciens strain lba4404 and gv3101 : pmp90 harboring expression vector pbgsb and pbglsb and co - cultured for 3 days in darkness on the culture medium ( ms + 0. 5mg / l 6 - ba + 0. 7 % agor ph 5. 7 )

    4以煙草(品種sr )無菌苗葉片為外植體,採用農桿菌浸染的葉盤轉化法,用構建好的植物表達載體對煙草進行遺傳轉化。外植體置於無抗生素的誘芽培養基( ms 0
  5. Northern blot results show that nos. 66 - 1, 84, 89 - 1, 97, 108, 152, 175 and 233 have stronger signal in sp6 - tester than in sp6 - driver ; and no. 23 has weak signal only in sp6 - tester, nos. 94, 165, 172, 185 and 191 have similar hybridization signals in both sp6 - tester and sp6 - driver ; nos. 4, 17, 18, 28, 6 9, 101, 156 - 1, 157 - 1 and 183 do not reveal hybridization signals in both sp6 - tester and sp6 - driver ; the results of sequencing and blastn and blastx on ncbi indicate that no. 23 cdna ( 846bp ) has significant alignments with nicotiana tabacum mrna for elicitor inducible beta - 1 - glucanase nt - sube76, and arabidopsis thaliana clone 7119 for glycosyl hydrolase family 17 ( protein id : at5g55180. 1, supported by cdna : 7119, supported by cdna : gi _ l 87001 54 ) and arabidopsis thaliana beta - 1 - glucanase - like protein ( gi _ 2 1594590 ) ; no. 84 cdna ( 560bp ) has significant alignment with lotus corniculatus aspartate aminotransferase mrna ( complete cds length = 1685, gi | 2605931 | gb | af029898. 1 | af029898 ) for aspartate aminotransferase ; no. 89 - 1 cdna has significant alignment with arabidopsis tha

    與同源性最高的擬南芥類似晚期胚胎發生高豐度蛋白比較,二者都具有lea 2結構域、保守分泌蛋白cog5608結構域和低復雜度區,都具有pkc磷酸化位點、酪蛋白激酶磷酸化位點、 n十四酞化位點和酚胺化位點,所不同的是: ( )在結構功能域上, 152全長cdna編碼的蛋白質序列中多了1個lea 2結構域、 l個保守分泌蛋白cog5608結構域和1個低復雜度區; ( 2 )在功能位點上, 152全長cdna編碼的蛋白質具有酪氨酸硫酸化位點、多了l個酪氨酸激酶磷酸化位點和1個可能的天冬氨酸富集區,但沒有n糖基化位點; ( 3 )擬南芥類似晚期胚胎發生高豐度蛋白的lea 2結構域具有顯著性( e
  6. Acbf is a transcription factor that combined with the ac - rich region of the promoter region. it has been cloned at arabidopsis thaliana, nicotiana tabacum and so on

    Acbf編碼了結合在苯丙氨酸解氨酶啟動子ac富集區上的一個轉錄因子, acbf基因已經在擬南芥、煙草等植物中得到了克隆。
  7. Rt - pcr showed that the gfp transcripts from wild type nicotiana benthamiana inoculated with the tnos deletion vector lacked a normal poly ( a ) tail

    接種野生型本生煙后的rt - pcr結果表明, gfp - atnos載體瞬時表達所產生的gfprna可能沒有poly ( a )尾序。
  8. Doubling efficiencies of the application dimethylsurfoxide in colchicines to treat haploids in tobacco nicotiana tabacum

    對煙草單倍體植株的染色體加倍效應
  9. I have used low copy pbin19 and single copy pmw755i5j binary vectors as backbone plasmids, to create a gene targeting insertion vector designated gfp tnos. after agro - infiltration into transgenic nicotiana benthamiana 16c, progeny were analyzed genetically for phenotypic changes, sirna accumulation, and dna methylation

    採用農桿菌浸潤法( agro - infiltration )感染轉基因本生煙16c ,並對同源基因瞬時表達所引起的植物表型變化、小分子rna的產生、 dna甲基化程度、以及相關性狀在後代中的遺傳情況進行了檢查。
  10. The high performance two - gene expression vector pc3c ! c2 was transformed to nicotiana tobacum honghuadajinyuan plants mediated by agrobacterium tumefaciens eh a105 according to the leaf disc procedure. transformed shoots were selected on solidified medium containing l00mg / l kanamycin

    將含有phac1和phac2雙基因的植物高效表達載體pc3c1c2 ,用凍融法,構建了pc3c1c2根癌土壤桿菌( agrobacteriumtumefacienseha105 )的工程菌。
  11. Nicotiana tabacum l. nc89 ' amp; jyh

    對2個不同基因型煙草品種
  12. Ssmapkk showed highest homology to the npk2 gene from nicotiana tabacum. genomic southern blot analysis suggested there was only one copy ssmapkk gene in suaeda salsa genome

    Ssmapkk與已報道的煙草npk2同源性最高, southern雜交表明該基因在鹽地堿蓬基因組中只有一個拷貝。
  13. Using conventional and immunoelectron microscopic methods, the cytomixis in pollen mother cells of tobacco ( nicotiana tobacum ) and the distribution of actin microfilaments during this process were investigated in the present study

    摘要應用普通電鏡和酶聯免疫電鏡技術,研究了煙草花粉母細胞中的細胞融合現象及細胞融合過程中肌動蛋白微絲骨架的變化。
  14. The s6 and 14 kd proteins could also suppress gfp silencing when pvx vectors harboring the two suppressors were infiltrated into nicotiana benthamiana whose gfp silencing had been induced by the tnos - deleted construct, and the suppression activity of the s6 protein was the strongest

    表型觀察和分子檢測表明, s6和14kd基因可以對gfp基因的沉默產生不同程度地抑制作用,且s6對同源基因dna的甲基化具有抑制功能。
  15. Finally, the full length cdna of real differential fragments were cloned by performing both 5 ' - and 3 ' - rapid amplification of cdna ends ( race ) and then the functional screening was performed by transferring the full length cdna into arabidopsis thaliana or nicotiana tabacum plant

    108號克隆尚未做測序分析。通過race技術對152和233兩個差異片段進行全長cdna克隆,獲得了152的全長cdna序列和233的3 』 。 cdna序列。
  16. In order to explore transgenic plants for production of recombinant scfv specific for presl ( 20 - 47 ), nicotiana tabacum was transformed with a gene encoding anti - presl of hepatitis b surface antigen scfv and bearing an / / - terminal endoplasmic reticulum protein signal peptide sequence

    在用煙草作為生物反應器時,分別將該單鏈抗體靶向細胞質和內質網。經westernblot分析,靶向細胞質中表達時,可溶單鏈抗體最高占總的可溶蛋白的0 . 06 。
  17. Three binary expression vectors were constructed, which harbored the cl - i - 2kl, r - 2kl, and d - l - 2kl - r - 2kl respectively, driven by the camv 35s promoter. then, agrofeac / erium - mediated transformation of iobacco ( nicotiana tabacum, w38 ) was carried out. transformed tobacco lines were obtained through tissue culture and confirmed by pcr and southern blotting analysis

    通過分子克隆技術,將c1 - i - 2k1 、 r - 2k1和二者的連接( c1 - i - 2k1 ? r - 2k1 )分別構建到雙元表達載體上,並使它們分別置於35s啟動子的驅動之下,然後通過農桿菌介導的技術分別對煙草( nicotianatabacum , w38 )進行了轉化,利用組織培養技術再生植株。
  18. Cbf1 gene was introduced into tobacco ( nicotiana tabacum ) by a plant expression vector pbi121 - cbfl containing the camv35s promoter using agrobacterium tumefaciens - mediated transformation. by pcr determination of cbf1 and kanamycin selection of transgenic progeny ( tl, t2 and t3 ), two pure transgenic lines were obtained. it was demon

    經c萬盧7基因的pcr鑒定和轉基因後代(包括tl 、 tz和t3代)的卡那黴素抗性分析,獲得2個單一位點插入的轉基因純系,證明了外源基因在轉基因煙草中的表達及遺傳。
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