northern blotting northern 中文意思是什麼
northern blotting northern
解釋
印跡,rna印跡-
The insulin - like growth factor - 2 ( igf - 2 ) and igf conjugated protein - 6 ( igfbp - 6 ) mrna level in rat calvaria bone tissue and mc - 3t3 - el cells were detected by northern blotting analyses and reverse transcription polymerase chain reaction. the estrogen responsive element ( ere ) in igfbp - 6 gene promoter was identified and involved in tcdd - reduced regulation of the gene expression by electromobility shift assays ( emsa )
正常胎鼠頭蓋骨組織igf一2mrna呈高水平表達狀態，而igfbp一6mrna的水平較低; ccf胎鼠頭蓋骨骨組織內igf一2mrna的表達較正常胎鼠降低， igfbp一6mrna的表達則明顯升高; atra和e :聯合應用時， atra可以抑制雌激素對細胞內igf一2和igfbp一6的這種調節作用。 -
These results demonstrated that merely examining the segregation of reporter gene in pollens is a reliable method that could avoid the laborious testing process of the pure line from transformed plants ; ( 3 ) the transcript levels of leetrl and leetrl were checked by northern blotting analysis
（ 3 ）兩個基因在反義植株里的表達都受到不同程度的抑制。在反義leetr1植株里， leetr1表達被抑制后， leetr2的表達也受到不同程度的抑制。同時，反義leetr2植株里也有相似的結果。 -
Northern blotting analysis showed that cryld mrna was more stable than crylab mrna, however, cryld mrna formed later 3h and less 3. 7 times than crylab mrna during the mid - phase of sporulation. moreover, crylab mrna could keep stable and large amount during the post - phase of sporulation. this suggested that difference of transcriptional efficiency and initiate time might be main reason for the difference of expression of crylab and cry id
結果表明：基因cry1dmrna的形成比基因cry1ab的mrna滯后3小時，且基因cry1d形成mrna的量很低，產生過程很平穩，在芽胞形成中期比cry1abmrna低3 . 7倍； cry1abmrna含量在芽胞形成前期高於後期，在後期仍能大量持續穩定地轉錄。 -
Total rna samples used in northern blotting were extracted from the light - exposed and light - interrupted cortex tissue of tuberous root of the two turnip cultivars
Northern分析採用的rna樣品，是從上述兩品種的光處理和暗處理的肉質根部周皮層中提取的總rna 。 -
Herein, we used cloning, sequencing, northern blotting, in situ hybridization, in vitro translation and co - immunoprecipitation, aimed to investigate the expression of wnk genes and the function of wnk4 kinase
本實驗應用克隆、測序、 northern印跡雜交、原位雜交、體外翻譯和免疫共沉澱技術，旨在研究wnk基因的表達情況及wnk4激酶的功能。 -
In addition, the well retained stability and integrity of cell membrane of boea leaves might also be an important mechanism which make them resurrect well. by using mrna differential display, 5 desiccation sensitive cdnas, 52 desiccation - induced cdnas, 21 up - regulated cdnas, 14 down - regulated cdnas and 16 phosphate induced cdnas were obtained. the cloning, sequencing, homological blasting and northern blotting results of 5 desiccation - induced cdnas and 3 phosphate induced cdnas implied that signal transduction induced by desiccation, regulatory gene cascades and functional genes such as g protein, protein kinase, vp3 - and mad3 - like genes might be involved in dehydration in the resurrection plant boea hygrometrica
對其中5個脫水特異誘導表達牛耳草光合作廠j的脫水保護和復甦機理的cdna （包括可能與復甦能力有關的cdna ）和3個磷酸鹽處理誘導表達的cdna進行克險測序、同源性探測和northern雜交檢測表明，牛耳草脫水過程中誘導表達的基因可能涉及到脫水脅迫的信號轉導「蛋白、蛋白激酶等） 、調節基因的級聯作用（ vp3 ， mad3樣基因等） 、結構基因產物調節細胞結構（包括細胞質膜）在脫水脅迫中的穩定性等。 -
Northern blotting indicates that wnk1 is widely expressed in different tissues and has two transcripts, whereas wnk4 is expressed primarily in kidney
Northern印跡雜交表明wnk1廣泛表達在人類、大鼠和小鼠的各個組織中，有兩個轉錄子。而wnk4則主要在腎臟中表達。 -
Whether the gene was transformed into the arabidopsis was confirmed by pcr - southern blotting, southern blotting and northern blotting. 1. pcr - southern blotting showed that all kanamycin - tolerant plants had strong positive signals, and no signal was shown in wild type plants
Pcr結果擴增出1 . 4kb的特異性條帶，表明sod2已整合進擬南芥中， pcr - southern進一步證實了pcr結果的正確性。 -
By northern dot blotting, it also showed that the foreign gene could express in the transgenic plants
Northern斑點雜交表明，外源基因在轉基因植株中正常表達。
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