noti 中文意思是什麼
noti
解釋
諾蒂-
Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine
本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。 -
The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction
目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。 -
We will now commence a consideration of k genesis 1, noti xk some of the importante points associated with god ' s creative acts that are recorded here
現在我?可以開始思考世紀了，注意一些在創世紀中記載的重要環節。 -
After digested with ecori and noti, the obtained dna fragment was linked with ppic9k by t4dna ligase and then the recombinant plasmid was transformed into competent dhscc. after positive transformants were sieved out by pcr, digesiting analysis and sequencing were also used to confirm the positive result more
所得的dna片段經ecor和not雙酶切後用t _ 4dna連接酶與ppic9k載體進行連接，然後導入大腸桿菌dh5 ，用pcr法篩選陽性轉化子，並用雙酶切和序列測定方法鑒定重組質粒。
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