nucleotide 中文意思是什麼

Detection of fibroblast growth factor receptor 3 gene mutation at nucleotide 1138 site in congenital achondroplasia patients

先天性軟骨發育不全成纖維細胞生長因子受體3基因1138位核苷酸點突變的檢測

The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。

It also carries a specific nucleotide sequence, the anticodon.

它還帶有特定的核苷酸序列即反密碼子。

More and more fluorescent signal can be collected with the pcr reaction carry on. the method is more automatized and much less time consumption ( only 3 hours from nucleotide hybridization capture to result found )

這樣利用熒光信號積累可以實時監測整個pcr進程，實現了pcr擴增和核酸雜交以及熒光電信號放大檢測同步進行的自動化檢測技術；實時熒光pcr技術具有更大的優越性： l ）不需要pcr后處理，

The fixation of co2 carried out by these autotrophs, in addition to atp, requires reduced pyridine nucleotide

除atp外，由這些自養生物進行的co2固定，需要還原的吡啶核苷酸。

The complete nucleotide sequence of the mitochondrial genome of f. limnocharis was detailedly compared with those of 5 other amphibians. the nucleotide sequences of 22 trna encoded by 6 amphibians mitochondrial genomes were combined and aligned to the homologous sequences of the 11 veterbrate taxa. using teleosts as outgroup, the phylogenetic analyses results show that mp, nj and ml trees all strongly support the monophyly of living amphibians with respect to other living tetrapods and favor a sister group relationship for caecilians and salamanders

我們在測定了澤蛙線粒體全基因組序列的基礎上，與已知其它的5種兩棲類進行詳細的比較分析，同時選擇了11種高等脊椎動物的線粒體全基因序列，以硬骨魚類做外群，用22個trna基因合併數據進行系統發生重建分析，結果表明mp 、 nj和ml樹都強力地支持現生兩棲類動物為單系群並且蠑螈類和蚓螈類為姐妹群關系（自引導值分別為92 、 99 、 100 ） 。

Based on recent molecular phylogenetic analyses using nucleotide sequences of the encoding the large subunit of ribulose 1, 5 - bisphyosphate carboxylase / oxygenase ( rbcl ), hypodematium should be not included in the athyriaceae, it has closely related to dryopteridaceae. on the other hand, athyriaceae, thelypteridaceae, blechnaceae, onocleaceae and woodisaceae form a large clade, so it may explain that tryon & tryon ( 1982 ) and kramer & kato ( 1990 ) putting it forward as dryopteriaceae s. 1

運用cpdna基因組編碼的磷酸核酮糖羧化酶大亞基（ rbcl ）的基因序列測定而構建的系統樹，顯示蹄蓋蕨科、金星蕨科、烏毛蕨科以及其他科構成一條與鱗毛蕨科平行的分支，因此可以說明kramer & kato （ 1990 ）把蹄蓋蕨科放入廣義的鱗毛蕨科是不合理的。

And ha molecules were synthesized in vitro utilizing excessive active udp - glca and udp - glcnac sugar nucleotide precursors in the presence of a divalent mg2 + or mn2 + ion

經轉化大腸桿菌dh5a和iptg誘導表達，提取原生質膜在體外利用活性前驅物合成了透明質酸。以s

It is the particular sequence of the nucleotide blocks along the molecule that encodes the genetic information.

沿著分子核苷酸的特定序列為遺傳信息編碼。

The fixation of co2 carried out by these autotrophs, in addition to atp, requires reduced pyridine nucleotide.

除ATP外，由這些自養生物進行的CO2固定，需要還原的吡啶核苷酸。

In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。

The holoenzyme recognizes specific nucleotide sequences on the dna duplex(promoters).

全酶還能識別DNA雙鏈分子上的特定核苷酸序列（起動基因）。

The holoenzyme recognizes specific nucleotide sequences on the dna duplex ( promoters )

全酶還能識別dna雙鏈分子上的特定核苷酸序列（起動基因） 。

Nucleotide sequences homologous to oncornaviruses dna products have been observed in human leukaemic cells.

在人白血病細胞中，已經觀察到與腫瘤毒病DNA產物相同的核苷酸序列。

Cyclic nucleotide dependent protein kinases

環化核苷酸依賴性蛋白質激酶

Genome - wide detection and analysis of alternative splicing for nucleotide binding site - leucine - rich repeats sequences in rice

基因選擇性剪接的全基因組檢測及分析

Ligase an enzyme that catalyzes the bond formation between two substrates at the expense of the breakdown of atp or some other nucleotide triphosphate

連接酶：可將兩個底物結合在一起的酶，此過程需要atp或其他核苷三磷酸供能。

Influence of 3 ' untranslated region single nucleotide polymorphism of tlr4 gene on luciferase gene expression

位點突變對熒光素酶表達的影響

G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software

本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。

The nucleotide acids in both conserved sequences are mutative, even the length of the spacer is variable, and they both brought troubles in determining escherichia coli promoter with computer

由於保守序列中的堿基是可變的，而且間隔堿基的長度也是可變，這給大腸桿菌啟動子的計算機識別帶來了難度。