oligonucleotide probe 中文意思是什麼

oligonucleotide probe 解釋
低核苷酸探測劑
  • oligonucleotide : 低核苷酸
  • probe : n 1 【醫學】探針；探示器；取樣器；【物理學】試探電極。2 【醫學】（對傷處等的）針探，探查；刺探；...
  1. Calculate the distibution of the melting temperature of the oligonucleotide probe sets that affymetrix uses for its microarrays

    計算用於微陣列的基因表達譜、和基因分型研究技術平臺使用的、低聚核苷酸探針裝置的溶解溫度分佈。
  2. The results of the experiments indicates that concentration of aminosilane influences the fluorescence background of glass slide, and some factors affect immobile ratio of oligonucleotide probe, such as aminosilane treatment time, aldehyde treatment time, uv crosslinking energy, washing temperature and time

    研究表明，氨基化試劑濃度對玻片熒光背景有影響，氨基化試劑處理時間、醛基化處理時間、紫外交聯能量和洗滌溫度和時間等工藝因素影響寡核苷酸探針的固定率。
  3. First, glass slides having been rinsed will be treated with nh3h2o, aminosilane and aldehyde. second, the quality of pretreatment surface of glass slides can be tested through methods of fluorescence and afm microscope. in the end, the characteristic of probe immobile ratio for oligonucleotide on glass surface is obtained through researching the internal relation of these two methods

    實驗選用表面平整的德國玻片，將清洗好的玻片分別進行羥基化、氨基化、醛基化，採用熒光法和原子力顯微鏡法分別檢測玻片表面預處理質量，研究兩種檢測方法之間的內在聯系，從而確定表徵玻片表面寡核苷酸探針固定率的方法。
  4. The result of fluorescence show that the fluorescence intensity of the surface of the treated glass slide connect with the probe immobile ratio of oligonucleotide. the more oligonucleotide probes have been linked with active group, the stronger fluorescence intensity is. for the strongest fluorescence, the technical conditions is : treatment of 2 % aminosilane of 20 minutes, treatment of 5 % aldehyde of 24 minutes, uv crosslinking of 150mj and washing of 5 minutes at 20

    兩種檢測方法表明，當活性基團呈柱狀、分佈均勻且尺寸比較大（ 200nm ）時，有利於寡核苷酸探針的連接，且連接探針數量多，玻片表面熒光強度強，固定率高；當活性基團呈錐狀、分佈及尺寸不均勻（ 150nm （ 300nm ）時，連接的寡核苷酸探針數量少，玻片表面熒光強度弱，固定率低。
  5. 5. prepare the oligonucleotide microarray add 16 prepared probes into 384 - pole plate, meantime, add p2, low homologic probes and probe buffer in the same concentration as positive control, blank control and negative control respectively. spot microarray as designed by the spotting machine

    5 .寡核昔酸微陣列的制備以同等濃度的反義鏈引物咫作為陽性對照，以探針緩沖液作為空白對照，以無關探針作為陰性對照，與制備好的16條寡核昔酸探針，同時加人384孔板。
  6. Therefore, the determination of seb is very important for food hygienic analysis as well as for clinical analysis. nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology. the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule

    其中b型葡萄球菌腸毒素（ seb ）是一種通常條件下更穩定，毒性最強的毒素，而核酸雜交技術則是分子生物學和基因工程中最常用和最基本的方法之一，因此本論文以該毒素的產毒基因為檢測對象，以壓電石英晶體為敏感元件，以合成的寡核苷酸探針為識別分子，構建了用於seb基因檢測的壓電生物傳感器。
  7. To improve the performance of assays on the oligonucleotide microarray, the factors that influence the hybridization effects such as surface chemistry, probe size, spacer length, hybridization conditions etc were intensely studied and optimized

    為改善寡核苷酸晶元的分析性能，對影響晶元雜交結果的因素，如片基表面的化學處理、探針的長度、間隔臂的長度、雜交條件等，進行了深入的研究和優化。
  8. In the present experiment studies, an acute traumatic model of lateral cortical impact was employed to study expressive changes of microtubule associated protein - 2 ( map - 2 ), cyclooxygenase - 2 ( cox - 2 ), glial cell line - derived neurotrophic factor ( gdnf ), caspase - 3 mrna and protein after brain injury in rats. immunocytochemical staining, western blotting, nucleic acid in situ hybridization with an oligonucleotide probe and computer image analysis were used to detect the dynamic changes of map - 2 mrna, cox - 2 mrna, gdnf mrna, and caspase - 3 mrna in the cortex after moderate traumatic brain injury ( tbi )

    本實驗從自行設計大鼠腦損傷落體打擊器開始，先行建立了一個便於觀察和施加處理因素、控制性好、重復性好的動物模型，選用30g擊錘從25cm高處下落，沖擊應力d為355 . 09kpa ，打擊大鼠右頂部，造成中等程度的閉合性腦損傷，從病理形態學、組織超微結構觀察及微管相關蛋白- 2 （ microtubuleassociatedprotein2 ， map - 2 ） 、環氧合酶- 2 （ cyclooxygenase2 ， cox - 2 ） 、膠質源性神經營養因子（ glialcellline - derivedneutrophicfactor ， gdnf ） 、 caspase - 3基因及蛋白表達的時間性變化，詳盡系統地闡述腦損傷后各指標變化的時間規律性及表達差異可能的形成機制。
  9. The distribution and amount analysis of these bacteria in different layers of core sediment indicated that there was an intact cycle that coupled sulfur metabolism with methane metabolism existed in this area, which may be the microbial response to the environment because there was seldom similar bacteria detected from " manganese nodule " area sediment by dna - dna hybridization with specific oligonucleotide probe and 16s rdna clone library analysis

    而16srdna克隆文庫分析和dna - dna雜交的結果表明「結核」區沉積物中這兩類細菌數目很少，說明「暖池」區沉積物中的微生物群落結構特徵是對環境因素的一種響應，同時也可能是影響該海區深海及海洋環境的一個重要因素。
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