orf open reading frame 中文意思是什麼

orf open reading frame 解釋

  • orf : n. 【魚類】黑色金魚。
  • open : adj 1 開著的，開放的；可進入的，可分享的 （to）; 無蓋的，敞口的；敞開的；展開的；開的；開闊的，開...
  • reading : n 雷丁〈姓氏〉。n 1 閱讀；讀書；講讀；朗讀，講讀會，讀書會；（議會議案的）宣讀。2 讀物。3 學識；...
  • frame : n 1 機構；組織；系統。2 結構，框架，構架，骨架，骨骼。3 體格，身軀。 4 精神狀態，心情。5 【園藝】...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The dna sequence had a complete orf ( open reading frame ), which coded a protein of 479 amino acid residues. the protein sequence of enolase which contained the conserved domain, was homologue to enolase of other organisms. it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii

    並將其中的一個gus基因用目的片段烯醇酶基因替換，構建了可以在植物中高效表達的載體pcambia2301g一enolase ，成功地將其轉入根癌農桿菌eha105中，為下一步進行轉基因植物的研究作準備。
  3. Orf, open reading frame

    和開放閱讀框架
  4. Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector

    本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。
  5. The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size. computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70. 3 % that would encode a protein of 552 amino acids ( aa ). the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp

    對phz1754進行外切核酸酶（ exonuclease ， exo ）順序缺失，獲得單向長度漸減重疊的系列突變體，核苷酸序列測定顯示出該ecor - sal片段的精確大小為2299bps ， frameplot程序分析揭示出該區域一個完整的開放閱讀框（ orf ）的存在，其大小為1656bps ， g + c含量為70 . 3 ，編碼552個氨基酸，利用blastsearch程序將orf的核苷酸序列及推導的氨基酸序列與因特網上基因及蛋白質數據庫進行綜合比較，發現無論在核苷酸水平還是在蛋白水平上，該orf均與膽固醇氧化酶表現出同源性，而且與鏈黴菌膽固醇氧化酶同源性最高，說明該orf編碼膽固醇氧化酶基因。
  6. Pcr product was cloned to the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector, and e. coli bl21 was transformed by the recombinant plasmid for expression

    Pcr產物經純化、酶切后，按正確的閱讀框架定向克隆到表達性載體pgex - 6p - 1中谷胱甘肽轉移酶（ gst ）基因的下游。
  7. Nucleic acid sequences of azoreduclase were searched and blasted in genbank. a pair of primers based on the conserved regions were designed. a specific fragment was amplified by pcr from the plasmid of rhodopsedomonas palustris and sequenced. the sequence contained a complete 471bp orf ( open reading frame )

    脫色實驗證明沼澤紅假單胞菌（ rhodopsedomonaspalustris ）對偶氮染料有較強的降解能力，我們通過genbank搜索，對所獲得的所有偶氮還原酶基因在ncbi進行比對並設計引物，從沼澤紅假單胞菌質粒中擴增獲得了一條含471bp完整開放閱讀框架的序列。
  8. The complete human znf325 cdna sequence is 2697bp and contains a 1389 - bp open reading frame ( orf ) that encodes a 462 amino acid protein with 15 zinc finger c2h2 motifs. the complete human znf359 cdna sequence is 3270bp and contains a 1932 - bp open read ing frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs. the zfp28 cdna sequence is 4104bp and contains a 2076 - bp open reading frame ( orf ) that encodes an 868 amino acid protein with an n - terminal signal peptide, two krab domains and 14 c - terminal c2h2 zinc finger motifs

    Znf325長2697個堿基，含有一個長為1389個堿基的開放閱讀框，編碼一個長為462個氨基酸的蛋白質，它包含15個c2h2型鋅指結構； znf359長3270個堿基，含有一個長為1932個堿基的開放閱讀框，編碼一個長為643個氨基酸的蛋白質，它包含一個n -端krab結構域和16個c -端c2h2型鋅指結構； zfp28長4104個堿基，含有一個長為2076個堿基的開放閱讀框，編碼一個長為868個氨基酸的蛋白質，它包含一個n -端信號肽，兩個krab結構域和14個c -端c2h2型鋅指結構。
  9. The complete human znf323 cdna sequence is 3179bp and contains a 1221 - bp open reading frame ( orf ) that encodes a 407 amino acid protein with a scan domain and 6 zinc finger c2i12 motifs

    Znf323長3179個堿基，含有一個長為1221個堿基的開放閱讀框，編碼一個長為407個氨基酸的蛋白質，它包含一個scan框和6個c2h2型鋅指。
  10. A bt - e. coli shuttle vector pht315 was deleted its replication region of bt, then constructed a novel vector named pht315 - 1 which composed a multiple cloning site, erythromycin and ampicillin - resistance marker and could only replicated in e. coli. used pht315 - 1, a 5273 bps dna fragment carrying a novel bt plasmid replicon was isolated and registered in genbank as ay278324. sequence analysis showed that there were at least three orf ( open reading frame ) in the cloned dna encoding 501, 333, 183aas. orfl had 98 % identities with replicating related protein ori43 of bt strain hd263. the others were no homology to any published bt replicating related protein. after continuous cultured for 70h at 30 c without antibiotic selecting press. the stability of plasmid carrying cloned replicon in bt acrystalliferous mutant strain hd73 cry was more than 98 %. and growth curve also showed that the novel replicon was stable and could replicate normally

    進一步序列分析表明該復制區至少有3個較大的orf ，分別編碼501 ， 333 ， 183個氨基酸。其中orf1蛋白序列與hd263復制蛋白ori43的同源性為98 ，而另外兩個orf和genbank己公布的bt復制相關蛋白無同源性。 30連續培養72h ，復制區質粒在bt無晶體突變株hd73cry ~ -中穩定性達98以上， 30h生長曲線也表明該復制區能夠在bt中穩定復制和遺傳，對受體菌株無明顯不良影響。
  11. The complete human znf359 cdna sequence is 3270 bp and contains a 1932 bp open reading frame ( orf ) that encodes a 643 amino acid protein with an n - terminal krab domain and 16 c - terminus zinc finger c2h2 motifs

    Znf359基因長3271個堿基，含6個外顯子，在基因組dna上長18kb 。它編碼的蛋白質全長643個氨基酸，含一個krab框， 16個c2h2型的鋅指，在兩者之間還有一個典型的中間重復序列。
  12. Initially, a polyclonal antibodies was used to screen the cdna expression library to isolate pf40 gene from millet. sequence analysis revealed that the pf40 gene contained an open reading frame ( orf ) encoding a protein with 75 % - 35 % sequence identity to the zips ( zinc or iron transporter proteins ) gene family. software analysis of the pf40 gene character showed that pf40 protein had a eight trans - membrane region structure, pf40 protein was also rich in leucine ( 18. 4 % ) and the protein was a hydrophobic protein

    序列分析表明該基因具有一個開放閱讀框（ openreadingframe ， orf ） : genbank中查詢該基因編碼的蛋白與zips （ zincorirontransporterproteins ，離子通道蛋白）基因家族的蛋白有75 - 35的相似性；軟體的分析結果顯示該基因編碼的蛋白為一個有8個跨膜區的跨膜蛋白； pf40蛋白為疏水蛋白，以上的特徵也許是pf40基因發揮作用的基礎。
  13. The results of sequencing analysis show that the open reading frame ( orf ) of the cdna encodes a protein of 418 amino acids ( 45 kda ) that is significantly different from other published mammal zp3 sequences at a < wp = 7 > few sites. the result may have important implications for the elucidation of mechanisms of fertilization and the development of contraceptive vaccine based on lzp3

    將其與genebank中多種哺乳動物zp3cdna進行同源性比較后，發現草原兔尾鼠與布氏田鼠zp3cdna編碼的蛋白前體的某些位點顯著不同於其它哺乳動物，其後續研究可能有助於哺乳動物受精機理的闡明與免疫不育疫苗的設計。
  14. It was reported that cadherin - like receptors are most likely the main receptors of bt toxic protein. bt - r3, a gene of 5551, is a newly cloned cadherin - like receptor gene and its orf ( open reading frame ) encodes 1715 amino acids

    Bt - r3受體基因是吳志平博士在其博士論文研究中新克隆到的鈣粘蛋白類受體基因，它的cdna序列全長5551bp ，編碼1715個氨基酸殘基組成的bt - r3前體蛋白。
  15. We sequence the inserted gene fragment of the indentified recombinant clone. the result is : angiostatin gene orf ( open reading frame ) links with the orf in expression vector correctly. but the first base of the codon aaa coding for lys414 in plg kringle 4 domain mutates from a to g which leads lys change to glu

    隨后取通過上述鑒定的重組克隆菌，對重組子插入片段測序，結果為： as基因開放讀碼框與表達載體的讀碼框正確匹配相連，但在其kringle4區相當于編碼plg的lys ~ （ 414 ）密碼子aaa的第一位堿基由a突變為g ，導致相應的氨基酸殘基突變為glu 。
  16. The tk gene contained an open reading frame of 957 bp encoding a 318 aa protein. upstream of the tk orf, three putative gc boxes are located at positions - 22, - 166 and - 199. a potential poly a signal begins 110 nucleotides downstream from the termination codon at position 1306

    在tkorf上游- 22 、 - 166 、 - 199位存在3個gc框樣序列，在終止密碼子下游第110個核苷酸處的1306位存在有多聚腺苷加尾信號。
  17. The cdna sequence with 789 bp encodes an uncompleted open reading frame ( orf ) of 263 amino acids, which included a signal peptide of 18 amino acids, a proposed propeptide of 171 amino acids, a partial mature protein of 74 amino acids. the sequences of signal peptide and propeptide are very similar to ones from wannan agkistrodon acutus reported by fan chunyan, but it only contains partial amino acids of mature protein. the 800 bp is likely to be a part of gene encoding metalloproteinase agkistrodon acutus from guangxi province

    結論： 600bp產物和已報道的皖南五步蛇纖溶酶金屬蛋白酶氨基酸序列相比較，有90 . 6 %的同源性； 800bp產物中信號肽及前肽和已報道的皖南五步蛇金屬蛋白酶很相似，同源性為97 . 9 % ，但僅含有部分金屬蛋白酶成熟肽的氨基酸序列，推測此片段為廣< wp = 6 >西五步蛇金屬蛋白酶原基因的一部分。
  18. Sds - page and activity staining analysis revealed that all of clones 2, 5 and 9 exhibited a single active band with 83 - kd in size, whereas clone 3 exhibited four different bands with 64 kd, 70kd, 76kd and 83 kd after construcing a series of deleted subclones from the plasmid prepared from clone - 5, a complete nucleotide sequences comprising of 3, 205 bps wes determined. one open reading frame ( orf ) was found in this sequence, which was comprised of 2, 661 bps and could encode a polypeptide with 887 amino acid residues

    利用基因分析軟體genetyx對clone - 5質粒的核苷酸序列進行分析，並搜索日本dna數據庫（ dnadatabankofjapan ，簡稱ddbj ） ，推斷clone - 5質粒的幾丁質酶基因含有一個2 ， 261bp的開放閱讀框（ orf ） ，編碼一條887個氨基酸的幾丁質酶蛋白； orf上游有一個啟動子。
  19. After enzyme restriction and sequencing analysis, the nucleotide data had been further analyzed by antheprot 5. 0 and clutalw softwares. the analysis results showed that the cloned dna fragment had a longest open reading frame ( orf ) of 1035nt, it predicted to be encoded a 344 - aa protein with the molecular weight of 36kda

    應用antheprot5 . 0 、 clustalw等分子生物學軟體分析，顯示主要外膜蛋白前24個氨基酸是較強的疏水性區域，可組成信號肽，其與omp基因的同源率達96 ，氨基酸的同源率高達98 。
  20. 713bp and 700bp specific fragments were amplified by pcr and ligated into pgem - t easy vector. it was identified by restriction endonuclease digest analysis, pcr and sequencing that this fragment contained the complete open reading frame ( orf ) of the hc and ha gene

    擴增產物連接到pgem - teasy載體上，轉化入大腸桿菌jm109中進行藍白斑篩選后，用酶切、 pcr鑒定和測序的方法鑒定出重組陽性質粒（ pgem - hc和pgem - ha ） 。
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