orit 中文意思是什麼
orit
解釋
奧里特-
To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. orit from e. coli plasmid rk2 was also incoporated into the vector, therefore, pij903 derivatives can be mobilized from e. coli into streptomyces sp. fr - 008 by either bi - parental conjugation or by conjugation between streptomyces
為了在克隆完整基因簇時避免這兩方面的影響並能隨機地獲得dna大片段，我們將第二代yac載體pjs97 - pjs98改造成為適合於在白林泉鏈黴菌基因組中dna大片段的基因置換與克隆鏈黴菌中分析被克隆大片段功能的基因置換載體。 -
A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained
將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。 -
A fragment, containing 2. 0kb cloned 5. tenebrarius dna and reported genes of erme and xyle, was inserted in plasmid phz132 ( an e. coli - streptomyces shuttle plasmid incorporating orit from rk2 ) to construct disruption plasmid pzxb0l4. the plasmid was transformed into e. coli et12567 ( puz8002 ) to construct recombinant e. coli et12867 ( puz8002, pzxb014 )
將克隆到的鏈黴菌dna2 . 0kb片段以及報告基因erme 、 xyle插入到具有orit的大腸桿菌?鏈黴菌穿梭質粒phz132中構建接合轉移質粒pzxb014 ，並將其轉入大腸桿菌et12567 （ puz8002 ）中，獲得供體菌et12567 （ puz8002 ， pzxb014 ） 。
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