pcr analysis 中文意思是什麼
pcr analysis
解釋
pcr分析-
K01458, 1284bp ), we designed the primers and regard the pcr productions as the internal standard. then by using rt - pcr analysis, high levels of expression of emu ghrelin mrna were detectable in proventriculus were detected, low levels of expression of duck ghrelin mrna were also detectable in lung muscle ileum duodenum corpus striatum cerebellum brain stem and gizzard
K01458 ， 1284bp ）設計引物，以其pcr產物作為內標，分別以鴯鶓各個組織反轉錄產物為模板，通過多重pcr的檢測ghrelin的mrna在鴯鶓的各個組織中表達情況，結果發現ghrelin的mrna在鴯鶓ghrelin的mrna除在腺胃特異表達外，在肺、肌肉、回腸、十二指腸、紋狀體、小腦、腦干、肌胃也有少量表達。 -
Methods : ( 1 ) the segregation of foreign target gene in the t1 by histochmical gus assays ; ( 2 ) identification of pure line from transgenic tomatoes ( tl ) through examining gus expression in pollens in conjunction with pcr analysis of marker gene ( npt ) ; ( 3 ) the transcript levels of leetrl or leetr2 in anti - sense transgenic plants ; ( 4 ) the phenotypes of the transgenic plants in tomato during whole life cycle under ethylene - treated and non - treated conditions
本研究以反義乙烯受體leetr1 ， leetr2基因番茄t _ 0代種子為實驗材料，利用gus基因表達研究外源基因的遺傳規律，並藉助于pcr技術對目的和標記基因的鑒定獲得轉基因t _ 1代材料。利用gus基因在t1花粉中的表達鑒定獲得轉基因純合植株。研究了轉基因後代的生長發育模式、對外源乙烯敏感性，以及靶基因的表達特性，初步探明了它們在乙烯受體系統中的功能。 -
After that we determined the presence of angiostatin gene in the putative recombinant virus with pcr analysis
之後利用pcr分析是否獲得重組angiostatin的桿狀病毒。 -
Gus examinaton showed that the rate of transgene was higher. and pcr analysis, pcr - southem and southern analysis all showed that chs was intigrated into quamocliy pennata chromosome successfully
同時，還以dig標記的camv35s上的一段為探針進行southern點雜交和pcr - southern雜交。 -
The engineering bacterium which carried bcih i - chi and i - glu cdna was pcg - ii. two methods of agrobacterium - mediated and gene gun were used to transformate long ya lillium. the results of pcr analysis and southern dot blotting hybridization demonstrated that the chi a nd glu cdna have been intergrated into host genome. at the same time ; compared agrabactenum - mediated method with gene gun method, the transformation frequency of the former was 16. 7 %, while the latter was 50 %, so gene gun transformation method was suitable for long ya liiliwn
用攜帶有幾丁質酶基因和- 1 、 3葡聚糖酶基因的工程菌，通過農桿菌介導法和基因槍轉化法轉化龍牙百合，經pcr和點雜交檢測證明外源基因已經整合到植物染色體中。同時對農桿菌介導法和基因槍法進行比較，發現農桿菌介導法的轉化率為16 . 7 ，基因槍法的轉化率為50 ，因此可能基因槍轉化法更適于龍牙百合的遺傳轉化。 -
4. after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time, incubation time and co - culture time, we set up the system of high frequency transformation of tomato cotyledons. then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation, and the resistant plants to hyg were obtained. by pcr analysis on part of the putative transformants, we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants. 5. transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg. trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection, growth and bio - morphology analysis, hyg - resistant experiment and pcr analysis
通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因；人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草，並且獲得了hyg抗性植株，通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測，證明目的基因己經整合到煙草基因組中。 -
There are four imperfect repeats v - v - e - k - k - n / e - e of which the core sequence is similar to map ib of mouse. rna blot and rt - pcr analysis showed that this gene is expressed specifically in the mature pollen and can be classified as a late gene in pollen development
計算機軟體分析st901基因核苷酸、氨基酸序列的相似性，結果表明， st901基因編碼區與探針sb401 、與高賴氨酸基因sblr 、與番茄tsb具有較高的相似性，它們可能來源於馬鈴薯的一個基因家族。 -
Their frequencies of gus expression in pollens were 97. 755 % 0. 800 % and 96. 556 % 0. 600 % respectively. to verify these results, the self - pollinated progenies of these two putative homozygotes were examined in t2 by histochenmical gus staining of vegetative tissues and pcr analysis using specific primers
（ 2 ）通過對轉基因t1代的花粉gus基因表達檢測，快速鑒定和篩選得到了番茄純合植株，加速了純合體篩選的進程，證明此方法可靠，而且不會造成大量寶貴材料的浪費。 -
Pcr analysis indicated that all lines had been integrated of ssmapkk. northern analysis revealed the presence of expression of ssmapkk mrna in transgenic lines. in principle, ssvp overexpression can increase proton electrochemical gradients across the vacuolar membranes, which permit the secondary active transport of na + and solute molecules
理論上， ssop的過量表達可增加轉基因植株細胞跨液泡膜的質子電化學梯度，為次級轉運提供驅動力，從而增加可溶性物質和na十向液泡內的轉運，提高轉基因植株的抗旱和抗鹽性。 -
As sampling of bioaerosols in natural environments is likely to be associated with substantial contamination by a range of microorganisms commonly existing in an ambient air, an investigation of the specificity of detection by targeted pcr analysis is required
于真實環境中采樣生物性氣膠往往會被一般大氣中常存之優勢微生物所污染，故需針對特定微生物發展其特定聚合酵素反應技術相關條件。 -
Northern blot and rt - pcr analysis show that sh2a gene is ubiquitously expressed in many tissues with three transcripts. the aberrant expression of sh2a gene in some cancers was found. it suggests that sh2a gene relates to tumors
Rtpcr及northern印跡雜交發現sh人在多種組織中有表?二?達，有3個轉錄本，而且在癌及癌旁組織的比較中發現，腫瘤組織中表達較高，初步證明與腫瘤的發生有關。 -
Purple clones were picked out from the plate, which show br was expressed. pcr analysis told us that the mutant br gene was transformed into l33
Br基因的定點突變改造和突變基因在嗜鹽菌中表達系統的建立使我們可以研究br的各種突變蛋白。 -
Gene expression of neural related genes was identified by semi - quanti - tive rt - pcr analysis and genechip assay. 4 and 10 days after neural induction, gene expression pattern was analysed by genechips, which showed the expression of some neural stem cells and mature neurons specific and related genes, repectively. especially the expression of gabar and glutamate dehydrogenase ( gad ), which meant the induced cells could be gabanergic neurons
2 .基因晶元檢測到未分化escs 、神經幹細胞及成熟神經細胞的相關基因，並由半定量rt一pcr證實基因晶元檢測未分化細胞表達胚胎幹細胞特異基因;誘導后第4天細胞高表達神經幹細胞特異性基因;誘導后第10天細胞高表達成熟神經細胞特異性基因，且有gaba受體、谷氨酸脫梭酶（ gad ）表達，說明誘導后細胞大多為以ba能神經元。 -
Pcr analysis of resistant seedlings with nptii gene primers showed that 6 out of 12 seedlings detected had the 700bp fragment specific to the plasmid pig121, indicating that t - dna had been integrated into the genome of sweet cherry
L ，負壓的適宜處理是lmmxio次。 6 、通過p r擴增，初步證明證明外源基回己轉入甜櫻桃。 -
In this study, we constructed two plant expression vector with rip gene from barley and linked to two promoters which have high promoting efficiency in monocotyledonous plants, respectively, and then transferred to rice mediated by agrobacterium tumefaciens. the main factors of affecting transformation were investigated and established its regeneration and transformation systems. pcr analysis showed primarily that rip gene had already integrated into rice
本研究構建了分別由單子葉植物中啟動效率較高的pact和p2 35s啟動子調控的大麥核糖體失活蛋白基因（ rip ）的植物表達載體，通過農桿菌介導法轉化水稻，經pcr檢測初步驗證了rip基因已導入水稻。 -
2. 3 gene expression identification of neural related genes by semi - quanti - tive rt - pcr analysis
3半定量rt - pcr在mrna水平檢測escs 、神經幹細胞及神經細胞相關基因。 -
Northern blot and rt - pcr analysis verified that the expression level of ldplcl was induced in germinating pollen, whereas ldplc2 expression level did not change during pollen germinating process. the interaction between heterotrimeric g proteins and plc was detected in a yeast two - hybrid system
Northern和rt - pcr分析，發現隨著花粉的萌發， ldplc2的轉錄活性提高，而ldplc1博士學位論文在萌發前後的轉錄水平沒有變化，推測至少ldplcz參與了對花粉萌發的調控。 -
Studies on cultivar classification of osmanthus fragrans by issr - pcr analysis
技術在桂花品種分類研究中的應用 -
Rt / pcr analysis showed that ams gene expresses in leaves, stems and flowers. the expression was n ' t detected in roots
Rt pcr分析表明ams基因在葉片、莖和花中表達，而在根中沒有表達。 -
Rt - pcr analysis was used to determine the levels of grp78 and grp94 mrna during the different phase of mouse development 2
Rt - pcr方法檢測發育不同時期小鼠胚胎腦組織中grp78 、 grp94mrna表達情況2
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