peptide sequence 中文意思是什麼

peptide sequence 解釋
肽序列
  • peptide : n. 【生物化學】肽；縮氨酸。
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  1. 2. the sequence of si gene from ibv - lx4 strain was consisted of 1614 bp from initiation codon atg to the possible cleavage site of spike glycoprotein, encoding for a 18 - amino signal - peptide with the n terminus of si protein and a polypeptide of 537 - amino acids. 19 highly conserved, potential glycosylation sites and 17 cysteines residues were characterized with si protein, homology analysis showe that there were gene deletion -

    S1基因：其全序列共1614bp （從起始密碼子atg到s前體蛋白裂解位點） ，編碼537個氨基酸，其氨基端有一編碼18個氨基酸的信號肽序列，第12 13位氨基酸殘基構成了信號肽的切割位點， 14 19位與111 124位氨基酸殘基為s1蛋白的跨膜區域。
  2. The protein accounting for the total was approximately 11. 80 %. the peptide was synthesized according to the sequence of grb - ast7. since ast7 is a hapten, it was conjugated to the carrier proteins bovine serum albumin ( bsa ) using l - ethyl - 3 - ( dimethyl - aminopropyl ) carbodiimide ( edc )

    根據ast _ 7氨基酸序列合成小肽，用edc做偶聯劑把合成的半抗原小肽與載體bsa偶聯成完全抗原，免疫家兔三次后，采血收集抗血清，用elisa測定效價為1 400 。
  3. We generated a recombinant eukaryotic gene expressing vector harboring a full - length hgh gene, 2. 4 kb genomic dna with four introns and the signal peptide sequence cloned to the eukaryotic gene expressing vector pcdna3. 0

    我們直接將含有4個內含子和自身信號肽的2 . 4kb全長人生長激素基因直接克隆至真核表達載體pcdna3 . 0 ，然後利用脂質體轉染家蠶bmn細胞，瞬時表達hgh 。
  4. The length of this phytase gene is1506bp interrupted once by an intron of 102bp in the 5 " part of the gene, this intron contains donor sequence - gtatgc, lariat sequence - gctgac and acceptor sequence - cag which are typically conserved sequence of the intron of fungal phytase gene. this gene encodes a peptide of 467amino acid residues with molecular weight of 51. 37kda, containing 13 potential n - glycosylation sites and a signal peptide sequence made up of 19 amino acid residues at n teminal of the peptide

    核苷酸序列分析表明， pcr擴增產物中包含有完整的phya基因，該基因全長1506bp ，其中包含一段長102bp的內含子，該內含子具有真菌植酸酶基因內含子的特徵保守序列： donor序列? gtatgc ， lariat序列? gctgac及acceptor序列? cag 。該基因編碼467個氨基酸，理論分子量為51 . 37kda ，其上有13個潛在的n -糖基化位點， n端19個氨基酸為信號肽序列，植酸酶活性位點序列（ crvtfaqvlsrhgaryptdskgk ）位於氨基酸序列的+ 71 + 93 。
  5. This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway

    測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。
  6. To target this mitochondrial enzyme into chloroplast, the cdna sequence of mnsod was fused to a chloroplast transit peptide from a pea rubisco small subunit gene, whereas expression of the chimeric gene was driven by the camv 35s promoter

    Pchlsod質粒含有煙草mnsod基因的cdna序列，與豌豆核糖體小亞基葉綠體引物肽（ tp ）的編碼基因序列構成融合基因，由35s啟動子調控。 npt基因為選擇標記基因， pgv2260為輔助質粒。
  7. A neurotoxic peptide ( named huwentoxin - v ) was purified from the venom of the spider by a combination of ion exchange chromatography and reverse phase hplc. hwtx - v has 35 amino acid residues, with the molecular mass 4111. 4da. the amino acid sequence has been determined as nh2 - ecrwylggcsqdgdcckhlqchsn - yewcvwdgtfs - cooh, which consists of 6 cys, formed three pairs of disulfide bridges

    本文報道從虎紋捕鳥蛛（ selenocoimahuwena ）粗毒中，結合陽離子交換和反相高效液相色譜分離純化出一種昆蟲毒素，命名為虎紋捕鳥蛛毒素- （ huwentoxin - ， hwtx - ） ；其天然突變體（ themutantofhuwentoxin ， mhwtx - ）也同時純化出來。
  8. ( 3 ) at post - translation level plant mutual sequence of starting translation aaca and eukaryotic secretory signal peptide sequence was added to 5 ' - flanking region of t - pa gene and kdel ( sequence located to endoplastic reticulum ) to 3 ' - flanking region by pcr amplication and plant expression vector pbemt was constructed

    （ 3 ）翻譯后水平通過pcr擴增的方式在t - pa基因5端添加了真核分泌信號肽序列和植物翻譯起始共有序列aaca ，在3端添加了內質網定位序列kdel ，構建了植物表達載體pbemt 。
  9. The new synthesized protein was led to endoplastic reticulum cavity by eukaryotic secretory signal peptide sequence and then anchored to innerwall of endoplastic reticulum by kdel sequence, which interdicted the process of protein entering golgi body and cytoplasm, and then avoided heterogeneous glycosylation modification of foreign protein and prolonged the disappearance of half life of protein in organism. 2

    真核分泌信號肽序列可以引導新合成的蛋白質進入內質網腔， kdel序列將進入內質網腔的蛋白質錨定在內質網內壁上，從而阻斷了蛋白質進入高爾基體和細胞質的過程，進而避免了外源蛋白質的異源糖基化修飾，延長了蛋白質在生物體內的半衰期。
  10. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  11. In comparison with genbank data, the homologies of the nucleotide sequence and amino acid sequence were as following : hc was 98. 4 % and 100 % ; ha was 97. 2 % and 99. 3 % respectively. 4. two recombinant prokaryotic expression vector were constructed which has complete open reading occlusing initation codon, leader signal peptide sequence and termination codon

    序列分析表明，所克隆獲得的基因與genbank中已經登錄的核苷酸和氨基酸的同源性分別為： hc98 . 4和100 ， ha97 . 2和99 . 3 ，證明本試驗蟲株與國外報道的同源性很高。
  12. Therefore, blys, its receptor or related antagonists may find medical utility in the treatment of b cell disorders associated with autoimmunity, neoplasia, or immunodeficiency syndromes. in this study, epo signal peptide sequence and hsblys gene were linked by soe method. the fusion product was cloned into eukaryotic plasmids. pcdna3, pcdna3. 1, pefneo, respectively. meanwhile, the epo signal peptide sequence was mutated so as to form a restriction enzyme cut site : bin i. thus the recombinant plasmid can be used as secreting plasmid expressing other gene

    本實驗通過3 』端互補，進行引物延伸合成epo信號肽序列：信號肽和hsblys基因採用重疊延伸拼接法形成融合基因；融合基因分別插入pcdna3 . 0 、 pcdna3 . 1 、 pefneo真核載體：引物延伸合成信號肽時，利用亮氨酸同義密碼，將信號肽基因的倒數第二個密碼突變，在重組載體上的信號肽序列之後，形成bln酶切位點，使三種載體成為分泌表達載體。
  13. Gfp gene was also fused behind the signal peptide sequence to construct plasmid p3301ubisiggfp and transformed to tobacco. the results of fluorescent detection showed gfp localization in the apoplast

    Elisa結果顯示，馬鈴薯蛋白酶抑制劑基因的信號肽序列使crylac基因在轉基因煙草中的表達量顯著提高。
  14. Comparison of the entire derived peptide sequence with other serine protease revealed significant homology, especially with a reported gene from another b. pumilus strain tyo - 67 ( 99 % homology )

    將克隆到的堿性蛋白酶基因（命名為ap ）編碼的成熟蛋白n一端序列與純化的脫毛蛋白酶n一端序列進行比較，二者完全相同。
  15. The quickly developing techniques of biological mass spectrometry ( bio - ms ) in recent years realized the high throughput identification of proteins by determining the accurate mass values of trypsin - digested peptides and the randomly selected peptide sequence tags, and have been successfully used in the studies of protein interactions and post - translational modification such as the phosphorylation

    摘要近幾年快速發展起來的生物質譜技術，依靠（酶解后肽段）精確質量數測定和隨機肽序列標簽分析，實現了對蛋白質高通量的鑒定，並被成功地用於蛋白質相互作用和蛋白質磷酸化等翻譯后修飾研究。
  16. To allow secretion of the crylac protein into the intercellular space, potato proteinase inhibitor ii ( pinll ) signal peptide sequence was n - terminally fused to the crylac coding region to construct plasmid p3301ubisigac

    運用pcr技術克隆了馬鈴薯蛋白酶抑制劑基因的信號肽序列，並將其分別連到crylac 、 gfp基因的5 』端，構建植物轉化載體p3301ubisigac和p3301ubisiggfp 。
  17. The signal peptide sequence which was used for secretedly expressing divergent gene in mammary gland cells was ligated to the e2 gene, the e2 gene with signal sequence was obtained by pcr. the gene resisting kanamycin was cut down from pgfp - cl vector and inserted into p22 vector, a p22 vector with gene resisting kanamycin was constructed, it was tried to construct an expression vector for transforming go

    豬瘟病毒ez基因的乳腺特異表達載體構建：將在乳腺細胞中特異分泌的信號肽序列連接到ez基因， pcr得到了目的片段，再將pgfpci載體上的kana基因切下與在乳腺細胞中特異表達的p22載體連接，使其帶有篩選標記，然後將帶有信號肽的ez插入到p22中，試圖構建山羊乳腺上皮細胞的特異性表達載體。
  18. In order to explore transgenic plants for production of recombinant scfv specific for presl ( 20 - 47 ), nicotiana tabacum was transformed with a gene encoding anti - presl of hepatitis b surface antigen scfv and bearing an / / - terminal endoplasmic reticulum protein signal peptide sequence

    在用煙草作為生物反應器時，分別將該單鏈抗體靶向細胞質和內質網。經westernblot分析，靶向細胞質中表達時，可溶單鏈抗體最高占總的可溶蛋白的0 . 06 。
  19. Expression of crylac in transgenic tobacco plants was assayed with elisa. the results showed that pinll signal peptide sequence enhanced the expression of crylac protein in transgenic tobacco

    熒光顯微觀察到gfp在植物細胞間隙高效表達， westernblot結果顯示crylac蛋白也在植物細胞間隙表達。
  20. To further understand its mechanism of internalization. methods and results 1. a new peptide sequence was designed based on the analysis of the molecular composed of the membrane penetrating peptides using the peptool life software

    最後在計算機photoshop軟體上，測量細胞熒光灰度值，並應用spss軟體進行統計分析，研究其穿膜能力與溫度、濃度、時間及細胞功能狀態之間的關系。
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