percent isolated 中文意思是什麼
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In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
After spraying the wax myrtle fruit with fluorescent powder and analyzing the resulting 11, 000 fluorescent bluebird droppings, the researchers found the feces were 37 percent more likely to occur in the connected patch than in the isolated ones, suggesting corridors do work
研究人員在散布附有螢光粉末的楊梅果實之後，收集了1萬1000個被螢光染色的知更鳥糞，加以分析，發現有接著走廊的碎片比沒有走廊的碎片，染色糞便多了37 % ，顯示這些保育走廊確實有效。 -
China, north korea ' s largest oil supplier, shipped 58, 685 metric tons of crude oil to its isolated neighbor in october, a 68 percent increase over the same month in 2005, the general administration of customs reported
中國是北韓最大的石油供應商，關稅總局報導，中國在十月份運載了58 ， 685噸原油至這個與世隔絕的鄰國，較2005年同期增加了68 % 。 -
" with economic growth has come centralization ; fully 76 percent of japan ' s 119 million citizens live in cities where community and the extended family have been abandoned in favor of isolated, two - generation households
譯：隨著經濟的增長帶來的集中化在1 . 19億人口中有76 %的人居住在城市，在那裡社區和幾世同堂的大家庭已經成為過去，而取而代之的是單門獨戶的兩代之家。
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