phage antibody 中文意思是什麼
phage antibody
解釋
噬菌體抗體[噬菌體蛋白與 免疫球蛋白的融合體，表達于噬菌體表面-
Then the biotin - labelled n - peptide interacted with the phage antibody library
對其研究可能有助於了解成癮機制和有臨床應用價值。 -
A murine phage antibody library containing 1. 710
成功構建一庫容量為1 . 710 -
The wells of elisa plate were coated with pab ( 100ng / l ) against h3n2, then phage was added to the wells. after incubation, the wells were washed vigorously with tbst to remove nonbinding phage. phage bound to the antibody were eluted with 0. 2mol / l glycine - hcl ( ph2. 2 ) for 10 min at room temperature and neutrialized with 2mol / l tris - hcl ( ph9. 1 )
以抗h3n2流感病毒的多克隆抗體（ 100ng l ）包被酶標板，加入制備好的肽庫，用tbst洗去非特異結合的噬菌體，加0 . 2mol l甘氨酸-鹽酸（ ph2 . 2 ） ，室溫放置10min以洗脫特異結合的噬菌體， 2mol ltris - hcl （ ph9 . 1 ）中和后，取2 l噬菌體接種大腸桿菌xl1 - blue菌進行空斑滴定，其餘噬菌體擴增後用于下一輪篩選，共重復3輪淘洗。 -
The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds. the last round enriched phage clones were used to reinfect
由鼠源噬菌體抗體庫淘選到展示有人絨毛膜促性腺激素單鏈抗體的噬菌體克隆。 -
Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。 -
Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )
Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。 -
Screening the antibody mimic peptide binding to hepatocellular carcinoma cells by phage display technique
用噬菌體表面展示技術篩選與肝癌細胞系結合的抗體模擬肽 -
One n - peptide - binding scfv clone was selected from the phage antibody library using affinity panning. the target antigen, n - peptide was biotinylated using photobiotin first
N -肽是一種新發現的神經肽，其n端與阿片肽高度同源，可與阿片肽受體相互作用。 -
The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis
取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。 -
Selection of anti - hcg scfv from murine phage antibody library selection of anti - hcg scfv from murine phage antibody library
從噬菌體抗體庫中淘選人絨毛膜促性腺激素的單鏈抗體 -
The phagemid particles displaying functional scfvs were rescued by reinfection of helper phage m13ko7, thus a murine antibody library was obtained
經輔助噬菌體m13ko7超感染回收全部重組噬菌體，此即噬菌體抗體庫。 -
Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。 -
Construction and screening of phage display single chain antibody library against bursaphelenchus xylophilus cellulase
抗松材線蟲纖維素酶單鏈抗體庫的構建及篩選 -
Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies
小鼠噬菌體抗體庫的構建和n -肽結合單鏈抗體的篩選 -
In the present paper the application of dna library, phage display random peptide library, phage antibody library, etc. in the research of serodiagnostic reagents and their special values were reviewed
本文綜述了基因文庫、噬菌體展示肽庫及噬菌體抗體庫技術在血清學診斷試劑研製中的應用及其各自的優點。 -
Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii, the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa
3 mg _ 7重組噬菌體抗體庫的淘篩用m13ko7輔助噬菌體感染轉化菌，以挽救出噬菌體形式的抗體庫；用高表達mg _ 7抗原的胃癌細胞系kato對抗體庫進行三輪淘篩；用elisa篩檢呈現有mg _ 7scfv的噬菌體單克隆。 -
The antibody induced by peptide gwyydal abolished vegf - induced huvec growth in dose dependent which specifically express the kdr. moreover, both of the peptide gwyydal and vasavfysalve displaying on phage also inhibited the growth of huvec
[結論]從噬菌體肽庫中篩選到了與抗體有高親和力的陽性噬菌體克隆，噬菌體表達的7 、 12肽gwyydal 、 vasavfysalve模擬了vegf的抗原表位，引起顯著的抗- vegf抗體反應。 -
Identification of the epitope of prrsv n protein monoclonal antibody with phage displayed random peptide library
蛋白單克隆抗體識別的表位 -
Identification of epitope recognized by monoclonal antibody with phage - displayed random peptide library
噬菌體隨機肽庫在確定單抗識別表位中的應用 -
The mechanism of ki 11 ing the schistosomulae in vitro depending on the specific ige antibody were observed. the distribution of the protein encoded by the cloned gene was determined by immuno - electromicroscopy with protein a - colloidal gold. in order to further analyse the epitope features of the protein relevant to the specific ige antibody, the phage display library of random peptides was screened by pooled sera with the specific ige antibody
藉助現行生物信息學分析手段，通過網際網路進入genbank數據庫，對sj43b與已知序列進行同源性比對，應用dnatoois軟體對其編碼蛋白序列的氨基酸紐成、親水性、抗原性和可及性進行分析，並在blastp程序下在genbank蛋白數據庫中對sj43b編碼3人卞醫科大學博士學杠論文蛋白進行同源性檢索。
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