phage gene 中文意思是什麼
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High stability of ptr102 - derived marking plasmids indicated that novel vector systems based on ptr102 could be constructed for the study on molecular biology and ecology of m. huakuii. 1kb gfp cdna fragment amplified by pcr was cloned into e. coli expression plasmid pet - hc, which was under the control of rna polymerase gene promoter from phage t7 with its own translation initiation codon
將pcr擴增的1kbgfpcdna片段克隆到大腸桿菌表達載體pet - 11c上，使gfpcdna在帶有lac操縱基因的t7噬菌體rna聚合酶基因啟動子的控制下、以自身的atg作為翻譯起始密碼進行翻譯。 -
Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv
方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。 -
Also the ci represser was expressed, presumably from its own phage promoter and prevented transcription from pr at low temperature. moreover, s. lividans strains expressing the c. 100 kda pks were different in sporulation and antibiotic production compared to strains without the 2. 7 kb pks gene, suggesting that the pks protein was active in an unknown manner in streptomyces
表達pks的變鉛青鏈黴菌菌株在孢子形成和抗生素產生方面與沒有2 . 7kbpks基因的菌株相比較是有區別的，因而推測在鏈黴菌中表達的雙功能結構域pks蛋白具有活性。 -
In order to provide valuable data for further exploring the immunogenicity and function of structural proteins of prrsv and designing epitoe vaccine for prrsv, the purpose of the research was to characterize the epitopes on structural proteins of prrsv bj - 4 by means of phage display technique, elisa and gene expression etc. 1. the epitopes and secondary structures of the structural proteins of prrsv bj - 4 were forecasted by molecular biology software goldenkey
本研究利用噬菌體展示技術，結合elisa和基因表達等技術對prrsv結構蛋白的抗原表位進行了鑒定與分析，為prrsv的結構蛋白免疫特性與功能研究以及表位疫苗的設計等奠定了基礎。 -
Two bifunctional streptomyces - e. coli vectors were constructed that contained the phage lambda promoter ( pr ) upstream of the his6 - tagged recombinant pks gene
構建了兩個鏈黴菌-大腸桿菌雙功能pks表達質粒，在重組pks基因上游攜帶有噬菌體啟動子。 -
The mechanism of ki 11 ing the schistosomulae in vitro depending on the specific ige antibody were observed. the distribution of the protein encoded by the cloned gene was determined by immuno - electromicroscopy with protein a - colloidal gold. in order to further analyse the epitope features of the protein relevant to the specific ige antibody, the phage display library of random peptides was screened by pooled sera with the specific ige antibody
藉助現行生物信息學分析手段，通過網際網路進入genbank數據庫，對sj43b與已知序列進行同源性比對，應用dnatoois軟體對其編碼蛋白序列的氨基酸紐成、親水性、抗原性和可及性進行分析，並在blastp程序下在genbank蛋白數據庫中對sj43b編碼3人卞醫科大學博士學杠論文蛋白進行同源性檢索。 -
It is firstly reported that the 85. 6kda heterodimeric penicillin g acylase from bacillus megaterium was processed and functional displayed on the surface of phage fd. phagemid psurfscript was used to clone full - lenghth gene with amber stop codon of penicillin g acylase ( signal peptide - subunit - interaal peptide - subunit ) from bacillus megaterium
革蘭氏陰性菌alcaligenesfaecalis的pga已在噬菌體表面獲得展示，並證實有活力，革蘭氏陽性菌來源的pga是否能在噬菌體表面成功展示，迄今尚未見報道。 -
Integrated plasmids containing phage lambda promoter pr - directed and epitope - tagged 2. 7 kb pks gene were constructed for tagging the natural fr - 008 pks with specific immunodeterminant ( epitope ). these constructs were transferred into streptomyces sp
構建了帶有噬菌體啟動子和特異性抗原決定簇（表位）及2 . 7kbpks基因的整合型質粒，用於標記天然的fr - 008pks 。 -
Effects of different leader peptides on the foreign peptide displayed on gene 8 protein of filamentous phage
不同的引導肽對絲狀噬菌體基因8蛋白展示外源多肽的影響
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