phosphogluconate dehydrogenase 中文意思是什麼
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6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page
將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。 -
It ' s pi is 5. 2 as determined with ief. amino acid composition analysis showed that one subunit of 6 - phosphogluconate dehydrogenase has about 480 amino acids and there are plentiful of ala, asp, leu, ser, glu, thr, phe, lys in it
氨基酸組成分析表明：枯草芽孢桿菌6 -磷酸葡萄糖酸脫氫酶的亞基由約480個氨基酸殘基組成，富含丙氨酸、門冬氨酸、亮氨酸、絲氨酸、谷氨酸、蘇氨酸、苯丙氨酸、賴氨酸。 -
Kinetic study shows that nadp + is the only valid coenzyme and that one substrate have no effect on the the other substrates " bind with the enzyme. products are competitive inhibitors, nadp + / nadph and 6 - phosphogluconate / ribulose - 5 - phosphate pairs are competitive whatever the concentration of the other substrates but noncompetitive versus the other substrates. therefore the kinetic mechanism of the 6 - phosphogluconate dehydrogenase is haldance - laidler - socquet random mechanism
兩種底物與酶的結合互不影響，而在產物抑制效果上， 5一磷酸核酮糖只競爭性抑制6一磷酸葡萄糖酸， nadph只競爭性抑制nadp + ，所以該酶催化機理是haidance一laidier一socquet隨機機理。
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