piezoelectric immunosensor 中文意思是什麼
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The second part, a renewable piezoelectric immunosensor is developed for the antibody of schistosoma - japonicum ( sjab ). after incubating 32 kd molecular antigens of schistosoma japonicum ( sjag ) on the qcm by applying the immobilization above, the nonspecific sites on the immunosensor are sealed by using bsa and nrs together. the immunosensor can detect the sjab with the linear range of 0. 54 ~ 32. 50 ug / ml
以感染兔血清為檢測對象，採用聚電解質吸附固定法，將日本血吸蟲分子抗原（ siag32kd ）固定於石英晶振表面，再以牛血清白蛋白（ bsa ）和正常兔血清（ nrs ）聯合封閉晶振上非特異性活性位點，可在0 . 54 32 . 50ug ml范圍內檢測感染兔血清中日本血吸蟲抗體。 -
The determination of human thymidine kinase ( htk ) in human serum, which is a key indicator of cancers can give information for the diagnosis and treatment of the malign diseases. the protein a layer was first self - assembled onto the gold electrode surfaces of quartz crystals, the monoclonal antibodies were then orientedly immobilized through the specific binding between the fc terminals of the antibodies and the self - assembled protein a. with this sensor, the affinity constant of antigen - antibody binding was estimated to be 1. 85 106 l / mol according to the scatchard ’ s plotting method, which proved the high bioactivity of antibody. finally, an amplified piezoelectric immunosensor was designed to determine the htk in
實驗中將蛋白a吸附於鍍金壓電石英晶體電極表面，用於定向固定htk單克隆抗體，成功研製了檢測htk的壓電石英晶體傳感器，並基於標準scatchard繪圖法，計算出免疫反應的親和常數為1 . 85 106l / mol ，證明該單克隆抗體具有較高的免疫活性;同時基於酶催化沉澱技術，設計了的檢測htk的質量放大壓電石英晶體傳感器，該傳感器可在0 . 1 - 10ng范圍內對htk進行定量檢測，應用此傳感器成功地對5種癌癥病人血清中htk的濃度進行了測定，實驗結果為癌癥的臨床診斷與治療提供了參考。 -
A piezoelectric immunosensor was developed by immobilized transferring antiserum on the surface of quartz crystal to especial answer transferring, it can be get good answer to determined in the range of 3. 92 ~ 79mg / l ; the adsorption and desorption of consult serum on the quartz crystal monitored with tr - antibody, determined the rate constant of immuno - reaction was 2. 14xl07 ( mol / l ) ' 1xs ' 1, the association constant of the immuno - reaction ( k1ss ) was 6. 75xl07 ( mol / l ) ~ l
Oz vomg l范圍內的參考血請中的轉鐵蛋白進行了定量測定，獲得很好的響應；利用石英晶體微天平技術在線監測了參考血清在固定了轉鐵蛋白抗體的石英晶振上的吸附與解吸過程，測得了其抗原抗體免疫反應的速率常數為2 14xl0 』 （ mol l ） 』 xs 「 『 ，結合常數為6 -
The detailed investigations are as follows : the first part of the focuses on the development of piezoelectric immunosensor which the immobilization of antibodies with protein a and the study. on kinetics of immuno - reaction using piezoelectric quartz crystal, and the second part is primarily directed to the development of piezoelectric immunosensor which the immobilization antibody with self - assembled monolayers ( sams )
具體內容如下：本文的第一部分著重於用蛋白a固定抗體的壓電免疫傳感器的研製以及利用壓電石英晶體對免疫反應的動力學研究；論文的第二部分著重於採用自組裝技術包埋抗體的壓電免疫傳感器的研究。 -
The main work is introcuced as follows : ( 1 ) the inorganic - organic nanocomposite of nanogold and chitosan, which have high biocompatibility, was synthesized in situ and used for the immobilization of antibodies. an amplified piezoelectric immunosensor for detection of normal human igg was designed with this nanocomposite film. comparing with self - assembling film of semi - cystamine, the inorganic - organic nanocomposite film could immobilize more antibodies with the activity of antibodis well retained
與半胱胺自組裝膜比較，納米金-殼聚糖復合物膜較大的比表面積使其可固載更多的抗體，同時可較好地保持抗體的反應活性;納米金的物理吸附作用使更多的沉澱結合於石英晶體表面，以其為載體制備的傳感器具有更大的頻率響應值。 -
A novel protein immobilization approach based on mercapto - pha sam for piezoelectric immunosensors has been proposed, which is formed by coupling mercaptoprorionic acid ( mpa ) to poly ( allylamine hydrochloride ) ( pha ) via 1 - ethyl - 3 - ( 3 - dimethylamino - propl ) carbodiimide ( edc ) and n - hydroxysuccinimide ( nhs ). after self - assembling a mercapto - pha sam on the gold electrode of the quartz crystal, the goat - anti - igm antibody is immobilized via cross - linking with glutaraldehyde. the detecting linear range of the piezoelectric immunosensor employing mercapto - pha sam is 0. 66 - 26. 4 ug / ml
應用偶聯試劑碳二亞胺（ edc ）和n -羥基琥珀酰亞胺（ nhs ）使聚丙烯胺（ pha ）與巰基丙酸（ mpa ）偶合形成巰基化的聚丙烯胺，應用這一新方法，實現了羊抗人igm抗體分子的固定，並在0 . 66 26 . 4 g / ml范圍內對人血清免疫球蛋白igm進行了測定。 -
Here, four kinds of immunosensor proposed as the potential alternatives to the clinical diagnosis methods are shown as follows : the first part, a novel piezoelectric immunosensor based on plasma - polymerized film ( ppf ) and polyelectrolyte is developed for the determination of transferrin in human serum
研製了四種與臨床疾病診斷密切相關的壓電免疫傳感器，具體內容如下：第一章研製了一種新的轉鐵蛋白壓電免疫傳感器。 -
It demonstrates that the results of clinical samples by this developed method are identical to those by the elisa method. 3. a novel piezoelectric immunosensor for the antitrypsin is developed based on the self - assembled menbrane ( sam ) of cystamine
同時對人血清樣品進行測定，將測量結果與酶聯免疫吸附法（ elisa ）進行對照，以f檢湖南大學碩士學位論文驗法考察顯示二者無顯著性差異。 -
The third part, considering that the present methods can only detect antibodies purified and its determining process is surely multifarious and time - consume, a simple and practical piezoelectric immunosensor is developed. it can be used for rapid, precise and on - line determination of activity of antibodies under label - free and purification - free conditions and has been tested for the anti - hsa in human serum
第三章針對目前檢測抗體的方法只能檢測純化的抗體，且操作繁瑣費時，研製了一種簡便實用的壓電免疫傳感器，用於在免標記和非純化的條件下快湖南大學碩士學位論文王樣2001年6月速、準確和實時檢測抗體的活性（效價） 。 -
The result suggests that comparing to the non - amplified piezoelectric immunosensor, an obvious improvement both in the sensitivity and detection limit is demonstrated by this developed method
L ） ；與不採用peg質量放大的情況比較，傳感器的測定靈敏度和檢測限均有較大改善，靈敏度為0 -
This thesis mainly finished two research tasks : ( a ) a spectral spr sensor has been developed to detect hbsag, ( b ) a piezoelectric immunosensor has been developed to dctect i ibsag
本論文的主要研究工作分為兩個部分。第一部分開展了spr傳感技術的研究與應用；第二部分開展了壓電免疫傳感技術的研究與應用。
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