pks 中文意思是什麼

pks 解釋
纏繞管
  1. In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover

    本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。
  2. You start referring to murderers as pks

    你開始用pk形容謀殺犯。
  3. In consideration of the colinearity between the activities of avermectin pks domains and structure of the polyketide product, the dh2 domain of avermectin pks, which corresponds to c - 22, 23 dehydration, appears to have partial dehydratase activity and this results in a mixture of c - 22, 23 double bonds ( " 1 " components ) and c23 hydroxy compounds ( " 2 " components )

    目前國內外尚未見有關僅產阿維菌素b1菌株的報道。根據阿維菌素pks基因結構與其聚酮合成反應步驟之間的線性關系，推測b2組分的產生可能是由於阿維菌素pks模塊2中脫水酶（ dh ）的不完全活性所造成。
  4. An expression vector carrying a fragment encoding the amino - terminal part of an fr - 008 type i pks module, containing a keto - synthase ( ks ) and part of an acetyl - transferase ( at ) domain was constructed for trial expression of the extremely high g + c content ( 76 % ) pks gene in plant

    為探索在植物中表達極高g + c含量的pks基因的可能性，構建了攜帶有編碼fr - 008型pks模塊氨基端部分的基因的表達質粒，包括一個酮基合酶（ ks ）和部分酰基轉移酶（ at ）活性結構域。
  5. In this study, we attempted to construct an engineering strain producing only avermectin bl through the replacement of dna encoding dh2 - kr2 domains of the avermectin pks ( avedh2 - kr2 ) with dna encoding dh2 - kr2 domains from the pikromycin pks in s. avermitilis olm73 - 12, producing only avermectins b and no oligomycin. gene replacement vector pxl201 ( pkc1139 : : 5 ' flank + pia : dh2 - kr2 + 3 ' flank ) was used to transform 5. avermitilis olm73 - 12 protoplasts

    我們以不產寡黴素而僅產阿維菌素b的工程菌olm73 - 12為出發菌株，用委內瑞拉鏈黴菌（ streptomycesvenezuelae ）中編碼pikromycinpks模塊2上完全活性的dh和酮基還原酶（ kr ）的dna區域對olm73 - 12染色體上編碼阿維菌素pks模塊2中dh和kr的區域進行取代，試圖構建僅產b1組分的基因工程菌。
  6. Streptomyces low copy number plasmid scp2 * is also employed for the cloning and recovery of large dna fragment from streptomyces. as scp2 * can accommodate large dna insertions and is efficiently transmissible among its permissive hosts such as s. lividansbut seemed to be unable to replicates in streptomyces sp. fr - 008, it might be a suitable vector for the cloning of the entire fr - 008 pks gene cluster through gene replacement followed by conjugation with 5

    Scp2 *可以在變鉛青鏈黴菌等許可宿主內復制並在許可宿主間有效轉移，但似乎不能夠在鏈黴菌fr - 008中復制，因此可能適合於在鏈黴菌fr - 008中通過進行基因置換及隨后的鏈黴菌fr - 008和變鉛青鏈黴菌間的接合過程來克隆完整的fr - 008生物合成基因簇。
  7. Using these vectors, expression of the pks gene was achieved both in streptomyces lividans and in e. coli in a heat - dependent manner, suggesting that the lambda promoter and temperature - sensitive lambda represser functioned in s. lividans as well as in e. coli

    利用這些質粒在變鉛青鏈黴菌和大腸桿菌中均表達出pks蛋白，兩種宿主中的表達都是熱依賴的。暗示噬菌體啟動子和溫敏型阻遏物在變鉛青鏈黴菌和大腸桿菌中都具有功能。
  8. The study on the function and mechanism of phrip1 is important for clarifying how the cell plate and cell wall form in plants. in this study, full length of phrip1 is amplified by pcr and ligated into pks plasmid, then the bait plasmid, peg202 - phrip1, is constructed. the inseret gene are sure to be translated into the right fusion protein through its sequence. in the yeast two - hybrid system, the bait plasmid ( peg202 - phrip1 ) and a reporter plasmid ( psh18 - 34 ) are introduced into the yeast ( egy48 ) by co - transformation. then cdna library ( which is in pjg4 - 5 ) is screened and two genes are obtained. the two insert gene fragments are sequenced. one of them is plastocyanin, the other is putative photosystem i reaction center subunit ii precursor, both of them are the necessary components of photosynthetic chain

    成膜素相關蛋白1 （ phrip1 ）是一個含608個氨基酸的蛋白質，它對于植物胞質分裂中細胞板的形成起到了十分重要的作用。研究phrip1的功能和機制，對在分子水平上闡明植物細胞板以及細胞壁形成的機理具有重大的生物學意義。在本實驗中，根據phrip1的序列設計引物對其進行pcr擴增，得到該基因后將其連接到了pks質粒上，並進一步構建成了誘餌質粒peg202 - phrip1 。
  9. Screening and characterization of marine bacteria with antibacterial and cytotoxic activities, and existence of pks and nrps genes in bioactive strains

    抗菌和細胞毒活性海洋細菌的篩選及其次生代謝基因證據
  10. Also the ci represser was expressed, presumably from its own phage promoter and prevented transcription from pr at low temperature. moreover, s. lividans strains expressing the c. 100 kda pks were different in sporulation and antibiotic production compared to strains without the 2. 7 kb pks gene, suggesting that the pks protein was active in an unknown manner in streptomyces

    表達pks的變鉛青鏈黴菌菌株在孢子形成和抗生素產生方面與沒有2 . 7kbpks基因的菌株相比較是有區別的，因而推測在鏈黴菌中表達的雙功能結構域pks蛋白具有活性。
  11. 100 kda pks protein but some of them did not give reproducible high expression in e. coli

    其中一些可超量表達具有預期分子量的pks蛋白。
  12. This vector containing the promoter of the cauliflower mosaic virus 35s rna for the expression of the 2. 7 kb pks gene

    該載體攜帶有花椰菜花葉病毒35srna的啟動子以指導pks的表達。
  13. Then the fr - 008 pks produced in e. coli was used as antigen to raise polyclonal antibodies against the natural fr - 008 pks

    接著將大腸桿菌表達的pks作為抗原制備與天然fr - 008pks對應的多克隆抗體。
  14. Integration through homologous recombination between the 2. 7 kb pks gene and the natural pks gene was confirmed by southern hybridization

    Southern雜交證實2 . 7kbpks基因和天然pks基因之間通過同源重組發生了整合。
  15. Two bifunctional streptomyces - e. coli vectors were constructed that contained the phage lambda promoter ( pr ) upstream of the his6 - tagged recombinant pks gene

    構建了兩個鏈黴菌-大腸桿菌雙功能pks表達質粒，在重組pks基因上游攜帶有噬菌體啟動子。
  16. Starting from level 160, players can participate in nation to nation pks and also will have ample opportunities to level regardless of whether the nation wins or loses

    從160開始，玩家可以加入種族戰，也會有大量的機會去升級，無論是否自己的種族是否應得戰爭。
  17. The detection limitation of pks using antibodies was 1000 fold higher than commassie blue staining, ready for detection of the probably very low level of pks expression in plant

    利用抗體檢測pks的檢出極限比考馬斯亮藍染色高1000倍，應可用於檢測植物中低水平的pks表達。
  18. Truncating of the 2. 7 kb pks gene from these expression plasmids resulted in production of an expected 50 kda protein, suggesting that these over expressed proteins were encoded by the cloned pks gene

    進一步截短的基因可以表達預期大小的50kda蛋白，表明這些超量表達的蛋白是由克隆在質粒上的pks基因所編碼。
  19. Pks expression plasmids containing strong, temperature - inducible, bacteriophage lambda promoters with or without the escherichia coli phage t7 promoter were constructed and some of them lead to high expression of the expected c

    為在大腸桿菌超量表達該2 . 7kbpks基因所編碼的兩個功能結構域，構建了具有不同啟動子組合的pks表達質粒。
  20. 70kb and 130kb in size respectively. though pks gene cluster for antibiotics fr - 008 was cloned, the genes for amino - mycosamine residue remained unknown. thus, attempt was also made to clone the desired gene ( s ), streptomyces sp

    首先從大量的發酵液中提取分離了鏈黴菌fr - 008產生的抗生素fr - 008 ，並通過大孔樹脂吸附、柱層析等手段進行了初步提純。
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