plant fragment 中文意思是什麼

plant fragment 解釋
植物碎片
  • plant : n 1 植物，草木 （opp animal）; 草本；〈商用語〉樹秧，苗木。2 莊稼，作物，收獲；（植物的）生育。3 ...
  • fragment : n. 1. 碎屑，碎片，破片，斷片。2. 未完稿，斷簡殘篇。vi. ,vt. （使）成碎片，（使）分裂。
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. Labeling tunel method. the cell ultrastructural changes were similar to apoptosis in animal cells : the apical meristemetic cells underwent the programmed cell death. this was first detected in the apex cells of apical meristem, while peripheral cells differentiated gradually into different parts of a floral bud. but all the cells in the floral bud were subjected to the pcd process before it developed into a complete flower. 140bp dna fragment was found to deposit in apical bud during the plant development. the most important role of caspase - 8 was detected by western blot, and the expression of the procaspase - 8 was time - related with the dna frgmentation and the transformation from vegetative to the reproductive growth. these results suggested that pcd was an active process during the differentiation of apical meristem, and the senescence observed in the apical bud was due to the pcd process

    顯微超微結構研究表明，短日照條件下豌豆頂芽的衰老過程是從營養生長錐向花芽的轉化，而用dna原位末端標記tunel caspase - 8 western blot和140 bp dna片斷積累的試驗結果證明，轉化為花芽的整個生長錐細胞發生了編程性死亡pcd ，而且其最頂端部分細胞首先發生pcd ，而頂端周圍的分生組織細胞逐漸分化出花芽的各部分，但頂芽最後並沒有發育成為完整的花，所有細胞就都發生pcd ，從而頂芽衰老。
  3. An expression vector carrying a fragment encoding the amino - terminal part of an fr - 008 type i pks module, containing a keto - synthase ( ks ) and part of an acetyl - transferase ( at ) domain was constructed for trial expression of the extremely high g + c content ( 76 % ) pks gene in plant

    為探索在植物中表達極高g + c含量的pks基因的可能性，構建了攜帶有編碼fr - 008型pks模塊氨基端部分的基因的表達質粒，包括一個酮基合酶（ ks ）和部分酰基轉移酶（ at ）活性結構域。
  4. By means of plant genetic engineering, foreign insects resistance gene can be transferred into plant cell. we cloned the cpti gene and transferred it into mustard by agrobacterium - mtdi & ted transformation method. and obtained the transgenic mustard plants. the main results are as follows : 1. isolation of cpti gene total rna was isolated from cowpea seedss cotyledons and leaves. the cpti gene fragment was amplified by rt - pcr using sequences of its two sides as primers

    本實驗是利用植物基因工程獲得抗蟲的轉基因芥菜植株，結果如下： 1豇豆胰蛋白酶抑制劑基因的分離分別提取豇豆種子、子葉及葉片的總rna ，逆轉錄成cdna 。以豇豆胰蛋白酶抑制劑基因兩端的序列為引物，用rt - pcr的熱啟動方法從上述cdna中擴增出目的基因片斷。
  5. In this study, a new approach of plant virus - resistance was expored by making up the cdna of ppiv. nib gene was amplified from plasmid pysr, which is plant expression vector containing potyvirus y nib gene. the nib gene was fused with the fragment encoding the mature part of papaya proteinase iv

    將番木瓜蛋白酶的cdna序列以全長、去除信號肽部分和成熟酶部分分別連接到細菌表達載體中，表達結果表明，只有含去除信號肽部分cdna的載體才能檢測到相應的表達的蛋白條帶。
  6. The identification of the phenylethanoid glycosides in plant extract of cistanche deserticola was based on the comparison of molecular ions, and the fragment ions obtained by ms ( superscript n ) experiments with those of the authentic standards and the data was reported in the literatures

    通過得到的分子量和碎片信息並與標準化合物以及文獻中的已知化合物比較，建立了這一類化合物快速鑒定的質譜新方法。
  7. Four green plants were regenerated form resistant calli of wen. 6 derived form 1500 implanted embryos. no green plant was regenerated form calli of 200 non - transformed embryos. pcr assays of 4 green plants showed that two of them attained the expected size of amplified dna fragment ( 1500bp )

    在含有20 - 60mg / l潮黴素的ms培養基上經誘導和繼代培養后，獲得了一批抗性愈傷組織，經過分化培養獲得4株再生苗，對照的200枚幼胚未獲得再生苗，對再生苗進行pcr檢測。
  8. ( 3 ) 490bp cdna fragment of fe ( ii ) - transporter gene mxlrtl was cloned by using common pcr method from iron - stressed root cdna library of malus xiaojinensis cheng et jiang with primers designed according to the conserved domain sequences of plant irt gene families. then we cloned the full cdna of mxlrtl gene by race method from this library with primers designed according to the sequence of 490bp cdna fragment

    （ 3 ）根據植物irt基因家族的功能保守區序列設計引物，首先通過常規pcr法從缺鐵脅迫處理的小金海棠根系cdna文庫中克隆了fe （ ） -轉運蛋白基因mxirt1的490bp的片段，然後根據測序結果設計引物，通過race法從該cdna文庫中克隆了mxirt1基因的cdna全長。
  9. We excised the pgn fragment, which contains a camv 35s promoter a gus gene and a nos terminator from the plant expression vector pbi121 with ecor i and hindlll and inserted it between the ecor i and hindlll sites of the plant expression vector pcambia3301, yielding pc3301 - pgn. the hf2 fragment was excised from pg - ho with xba i and sac i and then cloned between the xba i and sac i sites of the plant expression vector pc3301 - pgn to produce pc3301 - hf2

    將pbi121植物表達載體上含有camv35spromoter 、 gusgene和nosterminator的pgn片段酶切下來，連接到植物表達載體pcambia3301上，構建成中間載體pc3301 - pgn ，然後用hf2dna片段替換pc3301 - pgn載體上的gus基因，構建成植物表達載體pc3301 - hf2 ， hf2dna正向插入在植物表達載體35s啟動子的下游。
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