plasmid f 中文意思是什麼
plasmid f
解釋
f質粒-
1. expression of cry genes located in native plasmid in different flagella serotype strains to study cloning and expression of icps genes, an ecor i - f fragment of cryla ( a ) gene from pesi was inserted into pselect - 1 with t7 rna polymerase promoter in vitro. the plasmids of bacillus thuring fensisybt - 803 and ybt - 791 were analyzed by southern hybridization using an rna probe of ecori - f fragment and by pcr identification with cryl mixture primers
將cry基因的高保守區的cry a （ a ） ecog - f片段插入帶有t7rna聚合酶啟動子的質粒pselect - 1 ，獲得了能在體外轉錄的rna探針載體pbpl - 1 ，用該載體制備的rna探針具有特異強，背景清楚，省時省力等優點，已成功地用於蘇雲金芽胞桿菌的分子生物學研究和特異菌株的篩選。 -
The specific recombinant plasmid was identified by molecular weight, pcr and restriction endonuclease analysis. the results indicated that the resuctant construct contained the gene of interest f. the positive cloned was sequenced by sanger ' s dideoxy sequencing method. the results demonstrated that the f gene included an open reading frame which was 1662bp in length and encoded a protein of 553 amino acid
經核苷酸測序，該基因含一個1662bp的開放閱讀框，編碼553個氨基酸，與國外報導的新城疫各毒株f基因相比，核苷酸序列同源性為88 . 3 96 . 4 ，氨基酸同源性為92 . 1 96 . 4 。 -
The f gene from the resulting recombinant plasmid pcdna3 - f was sequenced
序列測定結果表明，克隆的f基因全長1668bp ，編碼553個氨基酸。
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