plasmid recombinant 中文意思是什麼
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First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。 -
The pcr product was inserted into expression plasmid pet - 32a ( + ) after restriction digest. then the recombinant plasmid was identified by endonuclease analysis, pcr ampliation and dna sequencing. the report showed that the recombinant plasmid had right open reading frame
重組質粒經酶切鑒定， pcr鑒定和測序，結果證實豬肺炎支原體黏附因子p97基因的抗原決定簇r1區定向插入了質粒pet - 32a （ + ） ，且閱讀框架正確。 -
Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量，我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上，構建ppic9k - fl表達質粒。 -
Study of the expression of exogenous augmenter of liver regeneration recombinant plasmid in liver tissue of rat
外源性肝再生增強因子重組質粒在大鼠肝組織中的表達研究 -
The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated
Niptg誘導表達，產物沉澱中於約45kda處顯見高合蛋白表達帶， westernblot分析表明，該蛋白帶可被篩庫血清中特異性lge抗體識別；而載體本身表達的26gst蛋白帶則否。 -
In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3
應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。 -
The recombinant plasmid puge dna and transfer vector pfastbacl dna were treated again in the same enzyme, were linked by means of t4 dna ligase and transformed into e. coli jm109 permissive cells, yielding recombinant transfer vector plasmid pfastbac - ge dna and were transformed into dhlobac containing vector bacmid
將重組質粒pugedna與轉移載體pfastbacldna用bamhi和ecori雙酶切處理， t _ 4dna連接酶連接，用連接產物轉化大腸桿菌jm109感受態細胞，得到重組轉移載體質粒pfastbac - gedna 。 -
The transformants were cultivated and the harvested cells were used in the procedure of conjugation by which the recombinant plasmid pid03 and pc05 were introduced into streptomyces avermitilis s - 2 respectively
經過在mym平板上的傳代和抗性標記的篩選分別得到了同源雙交換的菌株aved24和avecg 。 -
The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e. coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method. several conditions, which include diffend ph buffer and concelltheion of imidazole, were selected to purify recombinan nucleorotein
根據載體pproexhtb含有（ his ） 6特點，將融合蛋白進行純化，在純化過程中經各項條件的探索，確定為在裂解液中含有1mmpmsf的條件下，分別經過2倍體積的buffera和bufferb洗脫后，再收集ph5 . 9 ，含有80mmol / l咪唑的1倍體積bufferc洗脫液，可得到純化的融合蛋白。 -
Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2
含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。 -
In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate
將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。 -
97 % identities in amino acids respectively. the e. coli strain dh5 transformed recombinant plasmid phn was induced with 0. 6 mmol / m iptg for n gene expression. the expressed product was identified by sds - page and westem - blot test, a fusion protein about 47ku as we expected was found
將含有重組質粒phn的菌株dh5在37條件下培養，以濃度為0 . 6mmol / liptg誘導，重組質粒n基因phn融合蛋白獲得了表達：經sds - page ， western - blot試驗，確定其表達的融合蛋白產物大小為預期的47ku 。 -
The recombinant transfer vector pbacpak - hbmp was constructed by insertion of the hbmp coding sequences into the multiple cloning site of transfer vector pbacpak. 8. bmn cell line was co - transfected with pbacpak - hbmp plasmid and linearized baculovirus bacpak6 dna by dosper agent
將克隆到的hbmp基因通過適當的酶切插入到轉移載體質粒pbac - pak8的多克隆位點中，獲得重組轉移載體質粒pbacpak - hbmp 。 -
After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化，細胞裂解物的sds - page電泳， westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示：重組質粒轉染的細胞質中有棕褐色顆粒，而空載體轉染細胞及正常細胞無此現象；細胞裂解物sds - page電泳結果顯示：只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶，這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致； western - blot分析結果顯示：約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清，成蟲蟲體可溶性抗原免疫兔血清， ts87基因原核表達蛋白免疫兔血清（ ts87血清）以及一株具保護性的旋毛蟲單抗特異識別。 -
Construction of recombinant plasmid pires2 - egfp cck and its expression in hamster
質粒的構建及其在倉鼠體內表達 -
It has been reported that the eiav s2 is not essential and does not appear to affect virus infection and replication in vitro. thus, we introduced a his - tag into the s2 gene of an eiav infectious molecular clone recombinant plasmid ( pok8266 ) by using soe pcr method and obtained a new recombinant plasmid with his - tag, designated as eiav - pok8. 2 - his
本研究應用已構建好的eiav驢白細胞弱毒疫苗株的感染性分子克隆載體（ pok8266 ）為模板，通過soepcr方法在感染性分子克隆載體的s2基因獨特區內引入突變點，形成含有酶切位點（ nspv ）的突變體（ p1p4 ） 。 -
After linerization, the recombinant plasmid was transformed into pic hia pastoris by electroporation, which then were cultured in md plate free of histidine, from which the positive colones were propagated
重組質粒線性化后，用電擊法將重組質粒轉化入巴氏畢赤酵母，在缺組氨酸的md板上篩選陽性菌落，然後用不同濃度的g418 ? ypd板篩選多拷貝插入單菌落。 -
By blasting the homologous sequences in genbank databases, the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp. the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid. the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid, the pgcgh was transformed into e. coli bl21 competent cells
將得到的序列在genbank和embl數據庫中進行了同源比較，結果顯示：本研究克隆到的草魚gh基因與genbank中登記的x60474草魚gh基因有12個堿基的差異，編碼的氨基酸有3個氨基酸殘基的差異，同源性為98 ，影響蛋白質高級結構的保守二硫鍵為2個。 -
By recombinant dna techniques, the vp2 gene of gpv hi strain was fused in frame with 6his gene of prokaryotic expression vector pproex - htb. the recombinant expression plasmid of goose parvovirus vp2 gene pproex - htb - vp2 was transformed into e. coli dh5a and induced with iptg. sds - page analysis showed an induced expression product band about 72ku, which correspond to the sizes of vp2, reported in the literature
利用dna重組技術，將其結構蛋白vp2基因亞克隆至原核表達載體pproex - htb ， iptg誘導后成功表達出與預期大小相符的約72ku的融合蛋白，光密度掃描對表達產物進行初步定量，表明表達產物約占菌體總蛋白的14 。 -
Salmonella typhimuriwn, one of the invasive bacterial species, can be attenuated without loss of invasiveness and thus used for delivery of eukaryotic expression vectors into host cells in vivo. the recombinant plasmid containing the target gene is released inside the host cells and gain entry into the nucleus, resulting in expression of encoded antigens and subsequent induction of humoral and cellular immune responses
沙門氏菌（ salmonellatyphimurium ）是一種較為常見的侵襲性胞內菌，通過基因工程方法減毒后對宿主致病性顯著降低，但仍保留良好的侵襲力，可直接將真核表達質粒攜帶進入動物細胞內表達相應的蛋白而誘導特異性的免疫應答反應。
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