positive staining 中文意思是什麼
positive staining
解釋
陽性染色-
Explants were then transferred onto the selection medium containing 500mg / l carbenicillin and loomg / l kanamycin and incubated at 25, 16 / 8h light / dark cycle. small leaves of adventitious shoots differentiated from explants were cut and dipped into gus staining solution. positive shoots, gus tinted, were induced to root
分化出的不定芽切下半張葉片進行gus染色，呈陽性的植株從外植體上切下，在生根培養基（ ms 0刀sing lnaa 50mg幾kan ）中誘導根的形成。 -
In normal greenhouse condition, coda - transgenic wheat lines ( to ) had the same plant morphorlogy and pollen i2 - ki staining rate as untransformed control plant. after treating with 300 mm of 5 - fc, however, changes in configurations of spikelet, floret and anther have been observed in the transgenic lines but not in the control, and 50 % gus - positive lines displayed outside - opened glume, abnormal stamen, smaller and thinner anther, shorter filament, and failure of selfing. in parts of 5 - fc - treated transgenic lines, the pollen staining rate by i2 - ki was much lower than that of untransformed control
溫室栽培的轉基因小麥苗（ t _ 0 ）未噴5 - fc處理時植株外部形態和花粉碘-碘化鉀染色的著色率與未轉基因的對照沒有差異；用300mm的5 - fc處理后，發現有50 gus陽性株系與對照有明顯的區別，表現為小穗穎殼外張，花絲短縮，花藥發育不良，較小、黃白色且花粉粒少，自花不授粉，無外來花粉授粉則不結實。 -
When the resistant leaves budded, we select the teneral leaves for staining examination. 79. 5 % of 195 resistance germinations are tested positive. 3
待抗性葉片分化出芽時，取幼嫩的葉片進行gus染色檢測，在195株抗性芽中，有79 . 5的抗性芽gus呈陽性。 -
Half animals were perfused with paraformaldehyte following normal serum after test innnediatly, then removed the brain and fixed for 24 hours with paraformaldehyte, paraffin sections were prepared at 3um, histochemical staining for counting ce11 pro1iferating ratio and in situ hybridization for calculating integra1 scores of stem cell factor inrn positive signals
結果如下： 1增殖細胞的觀察經72h的快眼動睡眠剝奪，實驗組大鼠海馬齒狀回區brdu與p皿a （ proliferatingcellneuclearantigen ， pma ）陽性標記細胞數顯著增加。 -
Results 1. the expression of nf b p65 and i e in skin contusion repair in the control specimens, the positive staining of nf b p65 and i ba were in the basal cell layer, spinous layer, granular layer and sweat epithelium, sebaceous gland epithelium
實驗結果1 . nfkbp65與ikba在挫傷修復過程中的表達變化正常對照組皮膚中， nfkbp65與ikba在表皮基底細胞層、棘細胞層、顆粒細胞層和真皮層皮脂腺、汗腺上皮細胞呈陽性表達。 -
Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法：通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型，主要採用抗fos 、膠質原纖維酸性蛋白（ gfap ）和酪氨酸羥化酶（ th ） （或加壓素? vp ）免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法，系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核（ son ）神經元? ast超微結構的變化、延髓內臟帶（ mvz ）和son及下丘腦室旁核（ pvn ）之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。 -
P - selectin was negative both in the normal skin and in the postmortem damage group. in the wound specimens aged 1h, we detected expression of icam - 1 in the epidermis, a strong positive staining was observed in 3 hours after wound, and continued to 1 days from the skin was injured after incised, weak staining were showed on epiderm of the injury margin after 3 days incised, and disappeared in 5 days. expression of icam - 1 was also detectable in polymorphonuclear cells ( pmns ) in the wound specim - ents aged 3h
本實驗研究證明，皮膚損傷后表皮層icam1表達增高，其表達呈現一定時間規律性，在損傷后lh ，損傷邊緣表皮細胞即呈黃褐色染色，傷后3h組， icam一二在表皮層中呈深黃褐色，並一直持續至傷后id ，傷后3d組icam 1染色轉淡，呈黃褐色染色，在傷后sd及7d組， 1 m一二表達基本恢復至正常狀態。 -
After i. c. v. 6 - ohda, the c - fos expression of these areas induced by cold stress reduced significantly. 2 ) double staining showed that fos - like immunoreactive positive granules were observed in some vasopressin ( avp ) - immunoreactive positive neurons in paraventricular and supraoptic nuclei, as well as in some tyrosine hydroxylase ( th ) - immunoreactive positive neurons in nts and lc nuclei
（ 2 ）雙重染色顯示，寒冷應激誘導的fos樣免疫反應陽性顆粒可見于室旁核（ pvn ）和視上核（ son ）中的部分加壓素（ avp ）陽性神經元及孤束核（ nts ） 、藍斑（ lc ）中的部分酪氨酸羥化酶（ th ）陽性神經元。 -
The assay system of the biological activity of lymphotoxin was established using l929 cell as the sensitive target, lt international standard as the positive control and crystal violet staining method to detect viable cell after treated with lt. the best relationship between dosage and effect could be got if the cell seeding density in cell plate was 1. 6 0. 1 104 the dosage of amd was lug / ml, and the starting concentration of dilution in the plate of lt standard was 10 iu / ml with two fold dilution. the credibility of the established system was detected with rhtnfp developed by r & d
為確定經上述步驟純化后得到的目的蛋自lt 27的生物活性，本研究以l929細胞為靶細胞、淋巴毒素國際標準品為參照，採用結晶紫染色法檢測經淋巴毒素處理后存活的細胞，對淋巴毒素生物活性測定的細胞接種濃度、淋巴毒素標準品板上稀釋的起始濃度和梯度稀釋的倍數、放線菌素d的使用劑量等進行條件實驗后，建立了人淋巴毒素生物活性測定方法。 -
The positive staining were found in the mdv plaques and localized on the cytoplasme of the infected cell
表明大腸桿菌中表達的pp24融合蛋白保留有特異的抗原性。 -
The positive staining of hgc was first visible at the stage e2. 1 ( 2 hours before hatching )
免疫細胞化學染色結果顯示，鹵蟲hgc最早出現于孵化前2h ，于孵化后5h消失。 -
No markedly c - jun positive signals could be observed in the hippocampus of the two control groups. the distribution of c - jun positive cells expressed in psd group was consistent with that of apoptotic cells in he staining
在凋亡現象明顯cai和ca3區， c jun有大量表達；在凋亡細胞表達較少的caz和ca4區， c jun表達較少。 -
Then several kinds of selective k + channel blockers were applied to further clarify the specific type of k + channels involved in apoptosis. both bkca channel blockers, ibtx and chtx ( at the concentration of 100 nm ), significantly reduced neuronal death and number of positive tunel - staining cells and inhibited caspase - 3 activation
通道阻斷劑100nmibtx或100nmchtx均有顯著的神經元保護作用，可減少tunel陽性細胞比例及抑制caspase上激活；然而， a型鉀通道阻斷劑4apo0qm ）或skc 。 -
Objective to study clinicopathologic features and to analyze differential of malignant mesothelioma. methods a case of malignant mesothelioma of the mediastinum was observed with pathologic examination and the literaturenas briefly reviewed. results the malignant mesothelioma showed biphasic forms, fibrous component and neoplastic cells arranged in stream - or nest - or cabinet - like forms, immumohistochemical staining showed that the neoplastic cells were positive for vimentin, desmin, nse, cd99, cea, myod1. conclusion malignant mesothelioma of mediastinum is a relatively rare poor prognosis, with the combination of clinical histopathological and immunohistochemical data, its correct diagnosis and differential diagnosis can be made
惡性間皮瘤發病率很低，大部分發生於胸膜和心包膜1 ，此瘤臨床無特異性癥狀和體征，需與其他縱隔腫瘤相鑒別，方能作出正確診斷，現將1例縱隔惡性間皮瘤的臨床病理資料報告如下，並結合文獻對該腫瘤的形態結構特點、診斷與鑒別診斷、治療與預后進行討論。 -
Escs can be induced into neuron - like cells by sequential neural induction the undifferentiated escs grew in colonies, with clear bound. alkaline phosphatase staining showed dark brown positive particles were distributed in every esc colony. after the sequential neural induction, the cells converted into neuron - like cells, with homogeneous forms, which had round bright cell bodies and thin long bipolar or multipolar processes
5誘導分化前後細胞端粒酶活性檢測（ trap ）結果1 .使用序貫誘導法可將escs大比例誘導分化為神經元樣細胞未分化的escs聚集成團狀生長，細胞排列緊密，集落邊界清楚，堿性磷酸酶染色呈強陽性，胞漿布滿棕黑色顆粒。 -
All rats were killed on the 15th day. six samples of heart were chosen from each group for examining expressions of vegf, bfgf and the coagulation factor under light microscopy by immunohistochemical staining, and the quantitative analysis on positive responsive intensity of vegf and bfgf was conducted on the other 4 heart samples using the image analysing system, then mean microvessel density mmvd was calculated
術后第15天處死大鼠，每組選取6隻心臟標本，應用免疫組化法，光鏡下觀察大鼠心肌細胞vegf bfgf及因子表達情況應用圖像分析系統定量分析vegf及bfgf陽性反應強度，計算平均微血管密度mmvd 。
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