preadipocyte 中文意思是什麼
preadipocyte
解釋
前成脂肪細胞-
Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell
經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。 -
Disruption of lipid rafts and caveolae by depleting cellular cholesterol with cholesterol - binding reagent, - methylcyclodextrin or filipin, blocks the igf - 1 receptor signaling in 3t3 - l1 preadipocyte. both hormone induced adipocyte differentiation and mitotic clonal expansion are inhibited by lipid rafts and caveolae disruption. however, a nonspecific lipid binding reagent, xylazine, does not affect adipocyte differentiation or mitotic expansing
利用膽固醇結合試劑，如甲基環糊精和菲律賓菌素，減少細胞膽固醇的含量，破壞細胞膜上脂質筏/質膜微囊的結構，抑制了igf - 1受體信號誘導的3t3 - l1前脂肪細胞的分化以及克隆擴增。 -
Acylation stimulating protein inducing 3t3 - l1 preadipocyte differentiation
促酰化蛋白 -
Changes of gem gene expression during 3t3 - l1 preadipocyte differentiation
1脂肪細胞誘導分化過程中的表達變化 -
By counting cell, cell growth curve, cell doubling time, and the rate of the lipid filling of adipocyte to investigated the effect of pa on the proliferation and differentiation of preadipocyte
採用細胞計數、細胞生長曲線、細胞倍增時間、細胞充脂率等方法研究了pa對大鼠前體脂肪細胞增殖與分化的影響。 -
The dose of the 0. 10, 0. 15mmol / l cla had no difference. by mtt, determinied od after oil red o staining to investigate the effect of the cla, la, cla + la on the proliferation and differentiation of rat preadipocyte and adipocyte
通過mtt比色、油紅o染色od值測定等研究手段探討了cla 、 la 、 cla + la對大鼠前體脂肪細胞增殖與分化的影響。 -
Lipid rafts and caveolae are found to be essential for insulin - like growth factor ( igf ) - 1 receptor signaling during 3t3 - l1 preadipocyte differentiation induction. in 3t3 - l1 cells, igf - 1 receptor is located in lipid rafts and caveolae of the plasma membrane and can directly interact with caveolin - 1, the major protein component in caveolae
通過免疫熒光和純化脂質筏/質膜微囊的方法，發現igf - 1受體定位於脂質筏和質膜微囊中，而且igf - 1受體可以直接和微囊素1相互作用。 -
Changes of calcium calmodulin - dependent protein kinase gene expression during differentiation of 3t3 - l1 preadipocyte
1脂肪細胞誘導分化過程中的表達變化 -
By inducing 3t3 - l1 preadipocyte with individual hormone, it was identified that the mitotic clonal expansion was induced only by insulin, not by 1 - isobutyl - 3 - methylxanthine or dexamethasone
有報道用藥物抑制了克隆擴增的同時抑制了細胞的分化。我們發現克隆擴增是僅由胰島素誘導引起的。
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