pregnant mare 中文意思是什麼
pregnant mare
解釋
妊娠母馬-
Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e. metck darmstadt methods superovulation and collection of eggs for superovulation, female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ), and after 46 - 48 hours with human chorionic ginadotropin. ( hcg ). one - cell fertilized eggs were collected on the next day from oviduct of females
取4一5周齡成熟雌性昆明系小白鼠，腹腔注射pmsg （孕馬血清促性腺激素） 10iu ， 46一48小時后腹腔注射hcg （人絨毛膜促性腺激素） 1oiu ，將注射hcg后的雌鼠與8周以上的成熟雄鼠合籠交配，次日檢察陰栓，將查到陰栓的雌鼠處死，取輸卵管于mz培養液中，解剖鏡下撕開壺腹，釋放細胞團，然後用300林歲nil透明質酸酶消化去除顆粒細胞，口控吸管將卵細胞在m :中反復清洗，然後置於孵箱中，根據時間點收集g2期細胞。 -
In this study, we showed that the pdk inhibitor ly294002 regulated the cellular localization of materials 1. kunming mice were supplied from the department of laboratory animals, china medical university 2. pregnant mare serum gonadotropin ( pmsg ) and human chorionic gona - dotropin ( hcg ) were obtained from tianjin huafu biological products research institute and shanghai products research insititute, respectively
為了研究在小鼠受精卵中pkc 、 pkb是否也通過p21蛋白參與調控g2 m轉換，本實驗應用了pma和ly294002作為pkc 、 pkb的抑制劑，研究它們對小鼠受精卵中p21蛋白表達和定位，進一步探討可能的調控機制。
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