preinduction 中文意思是什麼
preinduction
解釋
隔代誘發-
To initiate neuronal differentiation, the preinduction media were removed, and the cells were washed with pbs then transferred to neuronal induction media composed of dmem - lg / 1 - 10 mm bme
開始誘導時,將預培養基倒掉,細胞用pbs清洗后,轉移人含有dmem lg l 10mmbme的培養基中。 -
Neuronal induction subconfluent cultures of mmscs were maintained in dmem - lg / 10 % fbs. 24h prior to neuronal induction, media were replaced with preinduction media consisting of dmem - lg / 20 % fbs / 1mm bme
2mmscs的神經誘導按以上方法制備細胞爬片,在神經誘導24小時前,培養基換成含有預誘導劑的培養基即合dmem ? lg 20 fbs lmmbme 。 -
Subconfluent cultures of rat were maintained in dmem / 10 % fbs, 24hrs before neuronal induction, media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ), to initiate neuronal differentiation, the preinduction media were removed, and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media, 5hrs later, 40ul dmso was given to every hole containing 2ml each
分別取第5代和第13代的mscs ,以8x10 cm 『濃度接種於六孔板中的蓋玻片上,制備細胞爬片,每孔加zinl培養液。達到80融合時,更換新鮮培養液,並在培養液中加人終濃度為lrnm的p一流基乙醇,誘導24小時, pbs洗滌,而後換成無血清的培養液。 -
In the later experiments dmem - lg / 20 % fbs / bfgf ( 50ng / ml ) was used as the neuronal preinduction media and dmem - lg / atra ( 150mg / ml ) was used as the neuronal induction media
在以後的實驗中, bfgf矚0n才rnl )被用來作為前誘導劑,人ttiho50略inl )被用來作為誘導劑。
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