primary cloning 中文意思是什麼
primary cloning
解釋
初步克隆化-
Now it has been one of the most important aquatic products in the freshwater cultivation. however, this prawn ca n ' t survive at a water temperature lower than 14c, which has seriously limited its cultivation expanding. in order to obta in a new breed of this prawn with increased cold - resistance, we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ), a sperm - mediated gene transfer technique
本研究的特色和創新之處在於，針對羅氏沼蝦不耐低溫，但體型相對較大，精莢明顯的特點，首次將目前已知具有最強抗凍活性的雲杉卷葉蛾（ sprucebudworm ， choristoneurafumiferana ）抗凍蛋白（ sbwafp ）基因（ sbwafp ） ，通過精子介導的轉基因技術整合到羅氏沼蝦的胚胎中，以期培育出耐低溫的羅氏沼蝦新品系。 -
The two mediums can meet primary culture and passage culture of the black bear fibroblast cells. the method of single cell cloning by micro - manipulating purifies fibroblast cells. as a result, steady fibroblast cells can be obtained and the rates of the cloning formation is 93. 75 %
通過單細胞集落顯微法純化細胞，可克服單細胞克隆中因細胞密度太低，難以形成克隆的缺點，而且操作簡單，效率高，速度快，其克隆率可達93 . 75 。
分享友人