primary culture 中文意思是什麼
primary culture
解釋
初級培養物-
A primary culture of myoblasts may be prepared by excising skeletal muscle tissue from 10 to 30 day old rat embryos.
制備成肌細胞的初級培養物，可以將10-30天的大鼠胚胎骨骼肌組織剪切下來。 -
Primary culture and morphological observation of gecko spinal cord cells
壁虎脊髓細胞的原代培養及形態學觀察 -
Primary culture of male germ cells in macrobrachium nipponense
日本沼蝦雄性生殖細胞原代培養方法 -
Primary culture and identification of icr mice sertoli cells
小鼠睪丸支持細胞的原代培養和鑒定 -
Improvement of method for primary culture of new natal rats cardiac myocytes in vitro
新生大鼠心肌細胞原代培養方法的改進 -
For the cryogenic preservation of fish, in this paper we made the primary culture of the kidney of allotetroploid crucian carp and primary studies were carried out on cryopreservation culture offish embryo cells derived from misgurnus auguillicaudatus or grass carp, the results of the experiments are as follows : the primary cell culture of the kidney tissue derived from allotetroploid crucian carp was carried out using tissue adherent culture, the primary observations of the growth conditions and morphology of the primary culture and subculture cells which originally come from the kidney tissue were also made
本文主要從魚類種質保存的目的出發，一方面以四倍體鯽鯉魚為材料，對四倍體鯽鯉魚腎臟組織進行初步培養，為建立相應細胞庫及下一步培養凍存的魚類胚胎細胞奠定基礎；另一方面，以魚類組織細胞培養技術為基礎，泥鰍胚胎細胞為材料，對魚類胚胎細胞凍存培養方法進行初步研究，並應用該技術方法對草魚早期胚胎細胞進行凍存培養實驗。報告如下：本文用組織貼壁法對四倍體鯽鯉魚腎組織進行原代、傳代培養。 -
Potato dextrose agar and grain medium were also used to identify fungi which were not determined by the primary culture. fungi were all secondarily cultured on sabouraud medium to observe the colony ' s texture, colour, growth rate, surface status and reverse pigment. the fungi should be examined by microscope to inspect their microscopic structure from 7th day to 21st day
使用的培養基有沙氏培養基、土豆培養基、真菌試驗培養基和5種種子培養基，連續培養4周，並隨時觀察菌落的色彩、生長速度、表面狀態、背面顏色等，並從第7天?第21天連續鏡檢以檢查真菌的顯微結構，綜合菌落形態和顯微結構，以確定真菌的種屬。 -
The results as follows : 1 ) the primary culture of qinchuan - scalper skin cells could be derived by fragment of tissue dispersed and cold digested single cell, collegenase i ( 150iu ml - 1 " ) and trypsin ( 0. 05 % ) being used at the same time is best to dissociate qinchuan - scalper skin tissue, which is appropriate to obtain qinchuan - scalper skin cells
組織塊法、分散單細胞及dispase冷消化法單細胞均能獲得好的秦川牛皮膚組織原代培養物。其中150iu ? ml ~ （ - 1 ）膠原酶i與0 . 05胰蛋白酶（ 1 ： 1 ）同時應用能更好的分離秦川牛皮膚組織，是獲得秦川牛皮膚細胞的適宜方法。 -
Primary culture of rat preadipocyte were prepared from the epididymal, inguinal and perirenal the fat pads of male normal, healthy, 15 - 20 days sprague - dawley rats. the preadipocyte grew better under the condition of 37, 95 % humidity, 5 % co2, ph 7. 0 - 7. 2, centrifuged at 1000r / min, m199medium, and 10 % fetal bo vine serum, seeded at a density of 4 l04, 5 l04, / cm2. oil red o staining was the special method to distinguish adipocyte from other cells, gimsa and he could determine the stage of the adiopcyte differentiation through the number of lipid drop, size and the position of the nucleolus of the staining fat cell
經過多次實驗，確定本實驗室大鼠前體脂肪細胞的最佳培養條件是：溫度為37 ，濕度為95 ， co _ 2濃度為5 ， ph值為7 . 0 7 . 2 ，離心力為1000r / min ，培養基為m _ （ 199 ）培養基，胎牛血清濃度為10 ，合適細胞接種密度為4 10 ~ 4 、 5 10 ~ 4個/ cm ~ 2 ，染色結果表明：油紅o染色是鑒定脂肪細胞的特異方法， gimsa和he染色可根據不同區域染色程度、著色差別判斷細胞核的位置及脂滴大小、多少，觀察大鼠前體脂肪細胞分化過程中的形態變化，進而確定脂肪細胞的分化階段。 -
Primary culture of hippocampal neurons of new - born rats
新生大鼠海馬神經元的體外原代培養 -
During the course of the primary culture and passage of es cells, co - culture of mouse es cells and mef was the best. in this research, cell digestive juice with 0. 125 % trypsin + 0. 02 % edta was relative gentle to cells
消化液濃度以0 . 125胰酶+ 0 . 02 edta對es細胞的損傷力最小，傳代后es克隆出現率最高。 -
Primary culture of ensheathing cells from neonatal rat olfacetory bulbs on the regenerated silk fibroin film
新生大鼠嗅鞘細胞在再生絲素膜上的生長 -
By this method of primary culture, cells and tissues could not only subsist for 11 months and more in vitro, but also be subcultured. these works lay a foundation of the establishment of silkworm embryonic cell line
為建立新的家蠶胚胎細胞系奠定了一定的基礎。原代培養的細胞和組織塊在體外有一定的生長現象，細胞體外最長存活達門個月以上。 -
There was about 7 days interval between every two passages. eg cell clones can be found in each primary culture from those embryos of appropriate age. in culture of one embryonic cell line, eg cell clones maintained after 9 passages
在實驗中，幾個適齡的胚胎的原代培養物中都出現了鳥巢狀的eg細胞集落，維持時間最長的一個胚胎細胞株在傳代培養了9代之後仍然可以觀察到eg細胞集落。 -
Cell adhesion to surface of the substrate is essential to development of the anchorage - dependent cells. only after adhering to surface followed by spreading can cells develop and proliferate. most synthetic polymers used as orthopaedic matrix substitute present hydrophobicity, which may correlates to the low degree of cell attachment. modification with cell adhesion protein / peptides can be benificial to the cell adhesion on polymers and then affect the cell proliferation and differentiation. cell attachment to substrate is primarily mediated by integrins, a widely expressed family of heterodimeric surface receptors. most extrcellular matrix proteins such as fibronectin, osteopontin, collagen type i, bone sialoprotein and vitronectin contain an arg - gly - asp ( rgd ) sequence which is specific to the fixation of cell membrane receptors like integrin. the main aim of this research is to measure, assess adhesion, proliferation of rabbit marrow stromal cells ( mscs ) on the polymers coated by fibronectin, collagen type i or biotie gen, which includes : ( 1 ) biologic characteristics of rabbit mscs were observed by two types of separating method in primary culture. ( 2 ) adhesion, proliferation and differentiation of mscs cultured on polymers coated with biotiegen were assessed. ( 3 ) also, adhesion, proliferation and differentiation of mscs were assessed on plga film or porous plga substrates coated with fibronectin, or collagen type i respectively. ( 4 ) bone formation was observed on the porous plga substrates coated with collagen type i in vivo. this research aims to give new way to make novel synthetic bone with cell adhesion and high bone induction capabilities
因此將這些蛋白包被、固定到材料表面，觀察骨組織工程種子細胞mscs細胞的粘附、生長特性是本研究的中心環節，並從以下方面進行探討： （ 1 ）採用不同原代細胞分離方法，研究其對mscs細胞的生物學特性影響。 （ 2 ）檢測基因勝肽膠對mscs細胞粘附、增殖及分化的影響。 （ 3 ）分別採用型膠原及纖維粘連蛋白（ fibronectin ， fn ）包被聚乙醇酸-乳酸共聚物（ poly （ 1actide - co - glycolide ） ， plga ）膜及多孔塊型plga材料，觀察細胞在單層或三維培養狀態下，型膠原及fn對mscs細胞粘附、增殖及向成骨細胞分化效應及能力。 -
Research, japan. there dr. jing helped to set up a primary culture system of neuroepithelial cells of day 10 mouse embryos. then he found a human teratocarcinoma cell line, pa - 1, secreted the growth factors related to egf and igf - i
曾於1989 1991年赴日本理化學研究所進行博士后研究；於1992 1993年以訪問學者身份赴日本理化學研究所進行合作研究； 1995年以訪問學者身份在法國strasbourg的遺傳與分子細胞生物學研究所（ gbmc ）進行合 -
At primary culture, pgcs co - cultured with their gonadal stromall cells were well grown. when subculture, we used primary chicken embryonic fibroblast ( pcef ), primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells. forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines
繼代培養時，經過反復實驗比較雞胚原代成纖維細胞飼養層（ pcef ） 、小鼠原代成纖維細胞飼養層（ pmef ） 、 snl細胞飼養層等三種不同飼養層的作用效果，最終發現以雞胚原代成纖維細胞製作飼養層同時添加各種生長因子最適合雞類eg細胞的生長，但pcef與pmef之間的差異不顯著。 -
The two mediums can meet primary culture and passage culture of the black bear fibroblast cells. the method of single cell cloning by micro - manipulating purifies fibroblast cells. as a result, steady fibroblast cells can be obtained and the rates of the cloning formation is 93. 75 %
通過單細胞集落顯微法純化細胞，可克服單細胞克隆中因細胞密度太低，難以形成克隆的缺點，而且操作簡單，效率高，速度快，其克隆率可達93 . 75 。 -
The results showed that : when cultured in the medium of m199, supplemented with 20 % bovine serum containing a moderate amount of antibiotics, the incubtion ph 7. 2 - 7. 4, the culture temperature 28. the primary culture cells formed the dense single wall within three weeks, the generation time of the subculture cells was 5 - 6 days, most cultured cells were fibroblastic - like cells with few epithelial - like cells
研究初步表明：在m199培養基加入20小牛血清（常規量雙抗） ， ph在7 . 2 - 7 . 4之間， 28的培養條件，四倍體鯽鯉魚腎組織原代培養三周左右即可形成緻密單層，傳代細胞為5 - 6天左右傳一次。培養細胞以成纖維樣細胞為主，有少數上皮樣細胞。 -
Research on the ratio method of measuring intracellular ca2 + concentration with ratio fluorescence imagemaster was reported in the last chapters, which include the folio wings : ( 1 ) the ratio experiment system of measuring intracellular ca concentration with ratio fluorescence imagemaster was established ; ( 2 ) the primary culture mode of suckling mouse cardiac muscle cell was established and the ca2 + concentration in suckling mouse cardiac muscle cell was measured. after adding ne ( norepinephrine ) to cardiac muscle cell, the relative fluorescence intensity was dramatic increased
本文後半部分主要圍繞用比率熒光光譜儀測量細胞內游離ca ~ （ 2 + ）的研究進行了以下工作： （ 1 ）建立了比率熒光光譜儀測量細胞內游離鈣離子濃度的實驗系統； （ 2 ）建立了乳鼠心肌細胞原代培養的模型，並對培養細胞進行了細胞內ca ~ （ 2 + ）濃度的測量。
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