primary germ cells 中文意思是什麼
primary germ cells
解釋
原生殖細胞-
Primary culture of male germ cells in macrobrachium nipponense
日本沼蝦雄性生殖細胞原代培養方法 -
Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示： eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f ）的es細胞培養液中形成典型的胚胎幹細胞克隆，堿性磷酸酶染色結果為強陽性，具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能，而且具有形成種系嵌合動物的能力。 -
The primary primordial germ cells is obtain from the human embryos after 4 - 8 weeks of pregnancy. after mechanical desection and enzymic digestion, the cells were cultured on mouse embryonic flbroblast or human embryonic flbroblast feeder layer inactivated by mitomycin. the medium contains several cytokines : lif ( leukemia inhibitory factor ), bfgf and foskolin
從4 ? 8周的流產胎兒的原始生殖嵴部位分離到原始生殖細胞，經過機械和化學方法的解離后培養於事先用絲裂黴素處理過的小鼠胚胎成纖維細胞或人胚胎成纖維細胞飼養層上，培養基中添加了lif ， bfgef ， foskolin等細胞因子，此後大約每7天左右傳代一次。 -
The expression and activity of cdc2 in spermatogenesis cdc2 is emergent in 10 days primary spermatocyte, but the expression is at a low level because the testicles are not right in scrotum, which can lead the germ cells stop to grow, however, the expression of cdc2 begins to increase since 20 days, which can improve the meiosis. the expression is also showed in sperm, which is the end of meiosis. the data showed that the phosphorylation of cdc2 tryl5 in germ cells is increase with
Cdcz場巧的磷酸化抑制cdcz的活性，它的磷酸化可以導致mpf復合物解離，使其失去生物活性，綜合以上實驗結果可以說明:在雄性小鼠生殖細胞發育過程中， cdcz在精原細胞、初級精母細胞及精子細胞中表達，隨著翠丸組織中生殖細胞的不斷成熟，生殖細胞中cdcz活性逐漸下降; cdcz在精子中同樣存在，但已失去其生物活性。
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