protein binding assay 中文意思是什麼
protein binding assay
解釋
蛋白結合測定法-
There were some degration in the purified protein, but gst - ap - 2 a still had the dna binding activity in the gel shift assay. the gst - testin and gst - antn1 were used for immunolize rabbits
雖然所提取的融合蛋白gst - ap - 2出現較多降解，但是電泳遷移率變動分析顯示其仍然具有良好的dna結合活性。 -
To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。 -
To identify the interaction between kyot2 and zo - 2 - i3, yeast two - hybrid system, purification of kyot2 protein and gst pull - down assay were performed in the experiments. after kyot2 and zo - 2i3 exchanged their vectors, yeast two - hybrid test revealed physical binding of the two proteins
本實驗通過酵母雙雜交及gstpull downas腳驗證了kyotz蛋白與zoz蛋白在體外的相互作用，並通過酵母雙雜交實驗初步確定其相互作用的位點。 -
By yeast two - hybrid assay, aes was found to interact with gp130 intracellular region through its conserved q domain. results from the yeast two - hybrid assay, gluthione s - transferase fusion protein pull - down assay and immuno - co - precipitation assay indicated that the q domain of tle1 is capable of binding gp130 intracellular domain, and the intracellular membrane proximal region of gp130 containing conserved boxl and box2 motifs seemed essential for this interaction. to investigate the consequence of this interaction, tle1 - gfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with gp130 expression vector
在通過酵母雙雜交分析確定aes通過q結構域與sp130分子胞漿區結合的基礎上，為確定tle1分子是否也能通過保守的q結構域與gp130分子胞漿區結合，我們通過pcr擴增編碼gp130胞漿區與tleq分子不同結構域的cdna ，構建了含有這些不同結構域的酵母雙雜交載體，通過酵母雙雜交分析證實： tle1分子通過其氨基端的q結構域與gp130分子胞漿區近膜段結合。
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