protein buffer 中文意思是什麼

protein buffer 解釋
蛋白緩沖系
  • protein : n. 【化學】朊，蛋白（質）。
  • buffer : n 1 【機械工程】緩沖器，緩沖墊；阻尼器，減震器；消聲器。2 【化學】緩沖，緩沖劑。3 緩沖者；緩沖物...
  1. On cytomembrane ; resolved the precipitation in buffer d ( contained triton x - 100 ) shattered by ultrasound, 4 vibrated for 60min, centrifuged at 45000g for 1 hour, took the supernatent to mesure protein concentration and then mesure the pkc activity

    目前，在鉛的神經毒性研究過程中，鉛對pkc的作用引起人們極大關注。這是因為鉛比鈣對pkc有更高的親和力。現已知， ph卜可以取代caz 」激活蛋白激酶c 。
  2. The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times, and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant

    用含有2mol l和4mol l尿素的包涵體洗滌液洗滌包涵體，在37條件下，洗去了大部分菌體蛋白及其它核酸物質。用8mol l尿素作為變性劑溶解包涵體，包涵體在8mol l尿素中的溶解性非常好。
  3. The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e. coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method. several conditions, which include diffend ph buffer and concelltheion of imidazole, were selected to purify recombinan nucleorotein

    根據載體pproexhtb含有（ his ） 6特點，將融合蛋白進行純化，在純化過程中經各項條件的探索，確定為在裂解液中含有1mmpmsf的條件下，分別經過2倍體積的buffera和bufferb洗脫后，再收集ph5 . 9 ，含有80mmol / l咪唑的1倍體積bufferc洗脫液，可得到純化的融合蛋白。
  4. This paper mainly discusses the source and chemical components of the oil - tea - cake, as well as the way and important value of its comprehensive utillization. we can extract tea oil and tea saponin, make acid picking buffer - corrodent, active carbon and protein feed from the oil - tea - cake. the producing methods and practical use of these products are presented

    本文簡要介紹了油茶餅粕的來源，主要化學成分及其綜合利用的途徑和重要意義，綜述了從茶粕中提取茶油、茶皂素，用茶粕制取酸洗緩蝕劑、活性碳及茶粕用作飼料的生產方法以及這些產品的實際用途。
  5. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  6. The expression vector pse380 - / iy / was constructed and transformed into e. coli dh5a, expressing hyl gene by adding iptg into the broth. the expression of hyl gene showed a 120kda protein band on sds - page gel and was found to have capability to degrade ha molecules derived from a microorganism dissolved in 0. 1 m acetate buffer solution ( ph4. 0 )

    經轉化大腸桿菌dh5a和iptg誘導表達後用sds - page電泳分析，獲得一條約120kda的表達條帶； iptg誘導表達后提取原生質膜測定透明質酸分解酶活力，表明該hyl片段的產物能夠在體外分解細菌來源的ha 。採用兩種策略滅活hyl基因。
  7. We did the same steps for three times, so we could get the extraction using the steps mentioned. laemmli sample buffer was added to the extracted protein, which were then boiled for 5 min. the protein samples were separated by 12 % sds - page and transferred to nitrocellulose membrane

    樣品蛋白經12 % sds一聚丙烯酞胺凝膠電泳分離后，轉印到硝酸纖維素膜上，與第一抗體4孵育過夜，經tbs洗滌后，再與第二抗體室溫孵育2小時， ttbs充分沖洗后，顯色觀察。
  8. The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane

    雙胸蚓組織中dna酶的分離純化雙胸蚓組織粗提取液經過選擇性酸變性、選擇性熱變性、硫酸按分段鹽析、 deae一纖維素（ de52 ）柱層析、超濾膜分級分離后得到一個電泳純的dna酶。
  9. To isolate and purify dnaase in the earthworm first, the tissue extract of earthworm was prepared by dissolving the earthworm with sucrose and denaturing the protein with low ph buffer. then dnaase was purified by denaturing the protein with higher temperature. the following steps were ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and filtration by ultra - filter membrane

    雙胸蚓組織中dna酶的分離純化採用蔗糖溶解雙胸蚓，並選擇性酸變性制備雙胸蚓組織粗提取液，再經選擇性熱變性、硫酸銨分段鹽析、 deae ?纖維素（ de52 ）柱層析及超濾膜分級分離對雙胸蚓組織中dna酶進行分離純化。
  10. Section hi : purify & refold recombinant hpk - 5 protein was efficiently expressed in e. coli jm109 as inclusion bodies. after bacteria were smashed by ultrasound, te buffer, 1 % ttiton x - 100 and 2 m urea was used to efficiently extract inclusion bodies

    用含sm尿素溶液洗滌包涵體， 8000r / min轉速下分離包涵體，能最大限度去除雜蛋白，同時不會降低目的蛋白的損失。
  11. Perturbations that could change the secondary structure of protein are introduced in this thesis. appling 2d correlation spectrum, peaks conformation and their assignments in the region of amide i and iii are completed to the bovine serum albumin in the phosphate buffer solution under alkaline ph - independent perturbation

    以牛血清白蛋白為例，應用二維紅外相關光譜技術，綜合應用酰胺帶和酰胺帶信息，對堿性ph擾動下的牛血清白蛋白二級結構在紅外光譜酰胺帶和酰胺帶中的信息進行了譜峰確認及歸屬指派。
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